Algorithm for navigated ESS.

ENT navigation has given new opportunities in performing Endoscopic Sinus Surgery (ESS) and improving surgical outcome of the patients` treatment. ESS assisted by a navigation system could be called Navigated Endoscopic Sinus Surgery (NESS). As it is generally accepted that the NESS should be performed only in cases of complex anatomy and pathology, it has not yet been established as a state-of-the-art procedure and thus not used on a daily basis. This paper presents an algorithm for use of a navigation system for basic ESS in the treatment of chronic rhinosinusitis (CRS). The algorithm includes five units that should be highlighted using a navigation system. They are as follows: 1) nasal vestibule unit, 2) OMC unit, 3) anterior ethmoid unit, 4) posterior ethmoid unit, and 5) sphenoid unit. Each unit has a shape of a triangular pyramid and consists of at least four reference points or landmarks. As many landmarks as possible should be marked when determining one of the five units. Navigated orientation in each unit should always precede any surgical intervention. The algorithm should improve the learning curve of trainees and enable surgeons to use the navigation system routinely and systematically.

Short tandem repeat (STR) variation in eight village populations of the island of Korcula (Croatia).

The aim of this study was to analyse short tandem repeat (STR) variation using the data on nine loci (D3S1358, vWA, FGA, THO1, TPOX, CSF1PO, D5S818, D13S317, D7S820) in the populations from eight villages on the island of Korcula, Croatia, in order to analyse its genetic and population structure. The analysis of STR data in this study indicated an appreciable degree of genetic homogeneity among the studied village populations on the island, even though a so-called 'east-west dichotomy' and differentiation between the inhabitants of the most recent settlement and the remaining ones was indicated with respect to the loci CSF1PO and TPOX, respectively. The validity of STR markers in assessing genetic structure of small populations and especially in determining the relationships among geographically closely related but reproductively isolated groups remains to be further evaluated, especially in terms of a larger number of studied loci in order to possibly find specific markers useful for resolving genetic patterns of variability at regional levels.

Barriers to health care for street youth.

This study investigates the barriers to health care faced by runaway adolescents. A convenience sample of 89 street youth located through community agencies was surveyed to elicit their perceptions of barriers to care. Results indicated that these youth experience a wide range of barriers to health care, both objective and subjective. They also experience fears with regard to receiving health care, many of which seem developmental in nature. The relative isolation of these youth compounds the objective barriers they face, yet many overcame these barriers and received needed care.

Preventing mental health problems in children with chronic illness or disability.

Children with special health needs have an added risk for mental health problems. These problems may be preventable in many cases by focusing on the development of competence in children and their family members in adapting to the chronic condition demands. Five strategies for developing competence are discussed: (a) encourage normal life experience, (b)increase coping skills, (c) increase use of social support, (d) coordinate the delivery of care, and (e) empower families. Examples of programs employing these strategies that have been evaluated are presented.

Family functioning of adolescents who parent and place for adoption.

While an extensive body of knowledge on adolescent sexual behavior and outcome has developed, current sociological and psychological studies of adolescents who place their babies for adoption are scant, often with inconclusive results. The level of family of origin functioning of adolescent parents and placers is even less frequently studied. This cross-sectional study of 84 adolescents attending an alternative high school for pregnant girls who recently made a placement or parenting decision assesses the family environment of teenagers in order to provide a broader understanding of these characteristics, which would assist practitioners in provision of services to this population. Using FACES II as the measure of family cohesion and adaptability, it was hypothesized that: (1) adolescent parents or placers would describe their families as being less functional than adolescent norms, (2) adolescents who placed their children for adoption would describe their families as being more functional than adolescents who parented their children, and (3) adolescents from the more functional families would report greater satisfaction with the placement decision than those from less functional families. Results indicate that Hypothesis 1 was supported, but contrary to Hypothesis 2, there were no significant differences in family functioning between placers and parents. Also, when comparing the discrepancy between adolescents' current and ideal family descriptions, no between-group differences were found. Because the vast majority of the respondents were satisfied with their placement or parenting decision, Hypothesis 3 also was not supported. Research needs and practice applications stemming from this study are discussed.

PIP: While an extensive body of knowledge on adolescent sexual behavior and outcome has developed, current sociological and psychological studies of adolescents who place their babies for adoption are scant, often with inconclusive results. The level of family or origin functioning of adolescent parents and placers is even less frequently studied. This cross-sectional study of 84 adolescents attending an alternative US high school for pregnant girls who recently made a placement or parenting decision assesses the family environment of teenagers in order to provide a broader understanding of these characteristics which would assist practitioners in provision of services to this population. Using FACES II as the measure of family cohesion and adaptability, it was hypothesized the: 1) adolescent parents or placers would describe their families as being less functional than adolescent norms; 2) adolescents who placed their children for adoption would describe their families as being more functional than adolescents who parented their children; and 3) adolescents from the more functional families would report greater satisfaction with the placement decision than those from less functional families. Results indicate that Hypothesis 1 was supported, but contrary to Hypothesis 2, there were no significant differences in family functioning between placers and parents. Also, when comparing the discrepancy between adolescents' current and ideal family descriptions, no between-group differences were found. Because the vast majority of the respondents were satisfied with their placement or parenting decision, Hypothesis 3 also was not supported. Data is based on 93 respondents to a mailed questionnaire in 1983 sent to 126 adolescent girls in suburban Minneapolis, Minnesota. 95% were Caucasian, which approximates the racial balance of this suburban, midwestern population. 59% ranked in the 2 lowest-social classes.

Heterochromatin composition and nucleolus organizer activity in four canid species.

Sequential staining with a counterstain-contrasted fluorescent banding technique (chromomycin A3-distamycin A-DAPI) revealed the occurrence of distamycin A-4,6-diamidino-2-phenylindole (DA-DAPI) staining heterochromatin in the centromeric regions of chromosomes 33, 36, 37, and 38 in the wolf (Canis lupus pallipes) and of chromosomes 13, 16, and 23 in the blue fox (Alopex lagopus). The red fox (Vulpes vulpes) lacked such regions. Staining with DAPI--actinomycin D produced a QFH-type banding pattern with clearcut differences in the staining behaviour of DA-DAPI positive regions between these three canid species. Staining with the fluorochrome D 287/170 did not preferentially highlight any of the DA-DAPI positive regions in any of them. Counterstain-enhanced chromomycin A3 R-banding and studies of nucleolus organizer region location and activity confirmed a close relationship between the karyotype of the wolf and the domestic dog. Few heterochromatic marker bands were encountered in these two species, but heterochromatin polymorphism was evident in the blue fox.

NOR activity, heterochromatin differentiation, and the Robertsonian polymorphism in Sus scrofa L.

The karyotype of the European wild pig (Sus scrofa scrofa L.) was analysed by means of silver-staining and the chromomycin A3/distamycin A/DAPI fluorescent banding technique to locate active NORs and to differentiate types of C-bands. The ribosomal RNA genes are localized at the secondary constrictions of chromosomes 8 and 10. All biarmed chromosomes, with the exception of chromosome 15/17 and the Y, had a chromomycin bright centromeric region that was moderately fluorescent with distamycin A/DAPI (DA/DAPI). Conversely, all acrocentric chromosomes and the Robertsonian fusion product (15/17) exhibited DA/DAPI bright centromeric heterochromatin. The results are compared with the chromosomal staining behavior of the domestic pig (Sus scrofa domestica L.) and discussed with respect to presumptive mechanisms of karyotypic evolution.

Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15, and the Y by D 287/170.

The utility of a newly synthesized chemical variation of DAPI (4'-6-diamidino-2-phenyl-indole), D 287/170, for differential staining of constitutive heterochromatin in man is demonstrated. Direct staining of human chromosomes with D 287/170 results in brilliant fluorescence of the paracentromeric C-band of chromosome 9, of a proximal short-arm segment of chromosome 15, and of certain heterochromatic regions in the Y. Bright, but less conspicuous fluorescence is occasionally seen at the centromeres of other chromosomes. The staining differentiation obtained by D 287/170 is very distinct, and the intensity of the fluorescent light is unusually high. The new fluorochrome should prove particularly useful for detecting and analyzing human chromosome 9 heterochromatin at various stages of the cell cycle in normal and structurally altered chromosomes.