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1.
J Neuropathol Exp Neurol ; 76(2): 126-134, 2017 02 01.
Article in English | MEDLINE | ID: mdl-28069929

ABSTRACT

Craniopharyngiomas are rare histologically benign but clinically challenging neoplasms. To obtain further information on the molecular genetics and biology of craniopharyngiomas, we analyzed a cohort of 121 adamantinomatous and 16 papillary craniopharyngiomas (ACP, PCP). We extracted DNA from formalin-fixed paraffin-embedded tissue and determined mutational status of CTNNB1, BRAF, and DDX3X by Sanger sequencing, next generation panel sequencing, and pyrosequencing. Sixteen craniopharyngiomas were further analyzed by molecular inversion profiling (MIP); 76.1% of the ACP were mutated in exon 3 of CTNNB1 encoding for ß-catenin and there was a trend towards a worse event-free survival in cases mutated at Thr41. Next generation panel sequencing of 26 ACP did not detect any recurrent mutations other than CTNNB1 mutations. BRAF V600E mutations were found in 94% of the PCP, but not in ACP. GISTIC analysis of MIP data showed no significant larger chromosomal aberrations but a fraction of ACP showed recurrent focal gains of chromosomal material, other cases showed loss in the chromosomal region Xq28, and a third group and the PCP had stable genomes. In conclusion, the crucial pathogenetic event appears to be WNT activation in ACP, whereas it appears to be activation of the Ras/Raf/MEK/ERK pathway by BRAF V600E mutations in PCP.


Subject(s)
Craniopharyngioma/genetics , Craniopharyngioma/pathology , Genomics , Mutation/genetics , Pituitary Neoplasms/genetics , Pituitary Neoplasms/pathology , Adolescent , Adult , Amino Acid Sequence , Child , Female , Genomics/methods , Humans , Male , Middle Aged , Young Adult
2.
Mol Metab ; 5(10): 807-822, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27688995

ABSTRACT

OBJECTIVE: Central melanocortin pathways are well-established regulators of energy balance. However, scant data exist about the role of systemic melanocortin peptides. We set out to determine if peripheral α-melanocyte stimulating hormone (α-MSH) plays a role in glucose homeostasis and tested the hypothesis that the pituitary is able to sense a physiological increase in circulating glucose and responds by secreting α-MSH. METHODS: We established glucose-stimulated α-MSH secretion using humans, non-human primates, and mouse models. Continuous α-MSH infusions were performed during glucose tolerance tests and hyperinsulinemic-euglycemic clamps to evaluate the systemic effect of α-MSH in glucose regulation. Complementary ex vivo and in vitro techniques were employed to delineate the direct action of α-MSH via the melanocortin 5 receptor (MC5R)-PKA axis in skeletal muscles. Combined treatment of non-selective/selective phosphodiesterase inhibitor and α-MSH was adopted to restore glucose tolerance in obese mice. RESULTS: Here we demonstrate that pituitary secretion of α-MSH is increased by glucose. Peripheral α-MSH increases temperature in skeletal muscles, acts directly on soleus and gastrocnemius muscles to significantly increase glucose uptake, and enhances whole-body glucose clearance via the activation of muscle MC5R and protein kinase A. These actions are absent in obese mice, accompanied by a blunting of α-MSH-induced cAMP levels in skeletal muscles of obese mice. Both selective and non-selective phosphodiesterase inhibition restores α-MSH induced skeletal muscle glucose uptake and improves glucose disposal in obese mice. CONCLUSION: These data describe a novel endocrine circuit that modulates glucose homeostasis by pituitary α-MSH, which increases muscle glucose uptake and thermogenesis through the activation of a MC5R-PKA-pathway, which is disrupted in obesity.

3.
Eur J Endocrinol ; 173(6): 853-62, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26392473

ABSTRACT

OBJECTIVE: Childhood craniopharyngiomas (CP) are often diagnosed after a long duration of history (DOH). Tumor size, hypothalamic involvement (HI), and obesity are associated with reduced overall survival (OS) and functional capacity (FC). The effect of DOH and specific symptoms in history on presentation at initial diagnosis and long-term prognosis are unknown. DESIGN: Retrospective analysis of patients' records and prospective longitudinal follow-up. METHODS: Histories of 411 CP patients recruited in HIT Endo, KRANIOPHARYNGEOM 2000 were retrospectively evaluated for DOH, symptoms, and characteristics. The effect of specific manifestations and DOH on clinical presentation and tumor characteristics at time of initial CP diagnosis and long-term outcome were analyzed. Main outcome measures were 10-year OS and progression-free survival (PFS), FC, and BMI during longitudinal follow-up. RESULTS: Median DOH was 6 months (range: 0.1-108 months) and correlated with age at diagnosis. Tumor size, HI, degree of resection, and BMI at diagnosis were not related to DOH. In multivariate analysis adjusted for age at diagnosis, only hydrocephalus was found to have a relevant influence on DOH. Visual and neurological deficits were associated with larger initial tumor size and impaired 10-year OS. Weight gain and growth failure were observed with longest DOH. PFS and FC were not related to any specific symptom. Endocrine deficits at diagnosis were associated with long DOH. CONCLUSIONS: CP is frequently diagnosed after long DOH, especially in older children. However, DOH was not associated with tumor size, HI, survival, or FC. Visual and neurological deficits necessitate rapid diagnostic workup.


Subject(s)
Craniopharyngioma/diagnosis , Delayed Diagnosis , Hypothalamus/pathology , Pituitary Neoplasms/diagnosis , Adolescent , Age Factors , Child , Child, Preschool , Cohort Studies , Craniopharyngioma/complications , Craniopharyngioma/therapy , Disease-Free Survival , Female , Follow-Up Studies , Growth Disorders/etiology , Headache/etiology , Humans , Infant , Infant, Newborn , Longitudinal Studies , Male , Nausea/etiology , Obesity/etiology , Pituitary Neoplasms/complications , Pituitary Neoplasms/therapy , Polydipsia/etiology , Polyuria/etiology , Prognosis , Prospective Studies , Retrospective Studies , Tumor Burden , Vision Disorders/etiology , Young Adult
4.
Eur J Endocrinol ; 173(3): 389-97, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26088821

ABSTRACT

OBJECTIVE: Hypothalamic obesity in childhood craniopharyngioma (CP) patients carries a high risk for development of metabolic syndrome. In metabolic syndrome, the development of nonalcoholic fatty liver disease (NAFLD) is known. The aim of this study is to detect the risk for NAFLD in childhood-onset CP. DESIGN: This cross-sectional study included liver computed tomography (CT); ultrasound analysis of abdomen; measurements of serum parameters, height, weight and body composition; and daily medication of patients with childhood-onset CP. METHODS: A total of 384 patients recruited in trials HIT Endo and KRANIOPHARYNGEOM 2000 were analyzed. Ninety-four survivors were included by fulfilling the criteria of proven hypothalamic involvement (HI), a minimum time interval of 5 years between diagnosis and study, and a minimum age of 18 years at the time of evaluation. A total of 19 patients agreed to participate. To quantify the degree of steatosis hepatis, analyses of liver density were performed once by non-contrasted CT of liver sections. RESULTS: NAFLD occurs in about 50% of CP patients with HI and is associated with elevated liver enzymes and homeostasis model assessment index. BMI is not an effective predictive factor but body fat mass measured by near-infrared spectroscopy (NIRS) is. Over half of CP patients (60%) with NAFLD are treated with stimulating agents, with risk of hepatic side effects. CONCLUSIONS: NAFLD is a major adverse late effect in childhood-onset CP. NIRS rather than BMI should be used to measure body composition and predict NAFLD. Stimulating agents for treatment of fatigue and daytime sleepiness in CP should be prescribed judiciously.


Subject(s)
Craniopharyngioma/complications , Fatigue/etiology , Hypothalamic Neoplasms/complications , Non-alcoholic Fatty Liver Disease/etiology , Pediatric Obesity/etiology , Pituitary Neoplasms/complications , Survivors , Adipose Tissue , Adolescent , Adult , Body Composition , Cohort Studies , Craniopharyngioma/surgery , Cross-Sectional Studies , Female , Humans , Hypothalamic Neoplasms/surgery , Hypothalamus/injuries , Liver/diagnostic imaging , Male , Metabolic Syndrome/etiology , Non-alcoholic Fatty Liver Disease/diagnosis , Pituitary Neoplasms/surgery , Radiography , Ultrasonography , Young Adult
5.
Neuro Oncol ; 17(7): 1029-38, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25838139

ABSTRACT

BACKGROUND: Quality of life (QoL) and long-term prognosis are frequently, and often severely, impaired in craniopharyngioma (CP) patients. Knowledge of risk factors for long-term outcome is important for optimization of treatment. METHODS: Overall survival (OS) and progression-free survival (PFS), body mass index (BMI), neuropsychological status (EORTCQLQ-C30, MFI-20), and psychosocial status were analyzed in 261 patients with childhood-onset CP diagnosed before 2000 and longitudinally observed in HIT-Endo. RESULTS: Twenty-year OS was lower (P = .006) in CP with hypothalamic involvement (HI) (n = 132; 0.84 ± 0.04) when compared with CP without HI (n = 82; 0.95 ± 0.04). OS was not related to degree of resection, sex, age at diagnosis, or year of diagnosis (before/after 1990). PFS (n = 168; 0.58 ± 0.05) was lower in younger patients (<5 y at diagnosis) (n = 30; 0.39 ± 0.10) compared with patients aged 5-10 years (n = 66; 0.52 ± 0.08) and older than 10 years (n = 72; 0.77 ± 0.06) at diagnosis. PFS was not associated with HI, degree of resection, or sex. HI led to severe weight gain during the first 8-12 years of follow-up (median BMI increase: +4.59SD) compared with no HI (median increase: +1.20SD) (P = .00). During >12 years of follow-up, patients with HI presented no further increase in BMI. QoL in CP patients with HI was impaired by obesity, physical fatigue, reduced motivation, dyspnea, diarrhea, and nonoptimal psychosocial development. CONCLUSIONS: OS and QoL are impaired by HI in long-term survivors of CP. HI is associated with severe obesity, which plateaus after 12 years. OS/PFS are not related to degree of resection, but gross-total resection should be avoided in cases of HI to prevent further hypothalamic damage, which exacerbates sequelae.


Subject(s)
Craniopharyngioma/epidemiology , Hypothalamic Neoplasms/epidemiology , Adolescent , Adult , Body Mass Index , Craniopharyngioma/complications , Craniopharyngioma/mortality , Craniopharyngioma/psychology , Disease Progression , Female , Humans , Hypothalamic Neoplasms/complications , Longitudinal Studies , Male , Neuropsychological Tests , Obesity/complications , Obesity/epidemiology , Young Adult
6.
J Pediatr Endocrinol Metab ; 28(1-2): 35-43, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25503864

ABSTRACT

BACKGROUND: As a result of hypothalamic involvement and/or treatment-related hypothalamic damage, up to 75% of childhood craniopharyngioma patients develop hypothalamic obesity. METHODS: Eating behavior was analyzed in 101 survivors of childhood craniopharyngioma, recruited from 1980 to 2001 in the HIT-Endo multicenter study, and in 85 body mass index (BMI)-matched healthy controls using the Inventory for Eating Behavior and Weight Problems (IEG) and the Inventory for Eating Disorders (ESI). RESULTS: Severely obese patients (BMI>8 SD; n=9) presented with pathological eating behavior, more weight problems, and eating disorders, as compared to obese (BMI 3-8 SD; n=44) and normal or overweight patients (BMI<3 SD; n=48). Craniopharyngioma patients with different degrees of obesity showed similar or even less pathological findings as compared to BMI-matched normal controls. CONCLUSION: Severe obesity is associated with pathological eating behavior/disorders in craniopharyngioma patients. As these disorders are not disease-specific, risk factors for hypothalamic obesity should be the focus of further craniopharyngioma research.


Subject(s)
Body Weight , Craniopharyngioma/epidemiology , Feeding Behavior/physiology , Feeding and Eating Disorders/epidemiology , Pituitary Neoplasms/epidemiology , Survivors , Adolescent , Adult , Age of Onset , Body Mass Index , Case-Control Studies , Child , Craniopharyngioma/complications , Craniopharyngioma/psychology , Feeding and Eating Disorders/etiology , Female , Humans , Male , Middle Aged , Neuropsychological Tests , Obesity/epidemiology , Obesity/etiology , Overweight/epidemiology , Overweight/etiology , Pituitary Neoplasms/complications , Pituitary Neoplasms/psychology , Survivors/statistics & numerical data , Young Adult
7.
J Clin Endocrinol Metab ; 99(11): 3972-7, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25077898

ABSTRACT

CONTEXT: Childhood craniopharyngiomas (CPs) are known to be associated with an increased risk of excessive weight gain and hypothalamic obesity. Atypical clinical manifestations include the development of a diencephalic syndrome (DS) with a failure to thrive or maintain weight at appropriate body mass index. CASES AND METHODS: In a retrospective study, we analyzed 21 of 485 childhood CP patients (4.3%) who presented with a low weight (< -2 body mass index SD) at the time of diagnosis. Eleven of the 21 patients were identified with a DS due to proven hypothalamic involvement. We show the clinical manifestations of DS and weight development before and after diagnosis in these 11 patients. The first significant differences between patients with low weight at diagnosis and normal-weight patients at diagnosis are observed at 5 years of age. Within the first 2 years after diagnosis, the weight of DS patients and normal-weight patients converge to a similar level. Tumor size does not play a role with respect to DS development. Finally, tumor characteristics of DS patients were compared with magnetic resonance imaging scans of obese CP patients at the time of diagnosis. CONCLUSIONS: DS is a rare clinical manifestation in childhood CP and should be considered as a discrete diagnosis in failure to thrive. DS at the time of diagnosis does not preclude weight gain after diagnosis of a CP with hypothalamic involvement.


Subject(s)
Craniopharyngioma/complications , Failure to Thrive/etiology , Hypothalamic Diseases/etiology , Pituitary Neoplasms/complications , Adolescent , Child , Child, Preschool , Craniopharyngioma/pathology , Failure to Thrive/pathology , Germany , Humans , Hypothalamic Diseases/pathology , Infant , Infant, Newborn , Pituitary Neoplasms/pathology , Retrospective Studies , Survivors
8.
Neurobiol Learn Mem ; 111: 71-80, 2014 May.
Article in English | MEDLINE | ID: mdl-24662774

ABSTRACT

Albeit histologically low grade (WHO I(o)) brain tumors, craniopharyngiomas and/or their surgical removal frequently affect the hypothalamus, amongst other brain regions at risk. Due to rich hypothalamic connections with prefrontal and limbic regions, hypothalamic injury may adversely affect neural substrates of emotion processing and higher cognitive control, including memory and executive functions. The current study is the first to investigate the consequences of hypothalamic involvement on neural substrates of emotional and cognitive functioning. Ten patients with childhood craniopharyngioma and known hypothalamic involvement and fifteen age- and intelligence matched control subjects (median age: 17.8 and 17.3 yrs.) were studied with functional magnetic resonance imaging and an emotional face recognition task. During encoding, participants were asked to classify neutral and emotional faces. In a subsequent recognition phase, participants had to recognize these old faces within a set of new faces. Behavioral performance was comparable between patients and controls. Neural activity revealed, however, differential recruitment of fronto-limbic brain regions during recognition. Patients exhibited an abnormal pattern of task-induced activation and deactivation in the anterior and posterior rostral medial prefrontal cortex and a higher functional coupling between anterior rostral medial prefrontal cortex and the thalamus. Additionally, we found a higher reactivity in the patients' amygdala to emotional relative to neutral faces when compared to healthy controls. Our data provide first evidence that hypothalamic damage impacts neural correlates of memory retrieval in medial prefrontal cortex, indicating a less efficient use of an area involved in executive control processes. We propose that the deactivation failure in the patients' anterior rostral medial prefrontal cortex is related to an increased coupling with the thalamus and reflects a reduced efficiency to flexibly adapt to task demands.


Subject(s)
Craniopharyngioma/physiopathology , Emotions/physiology , Hypothalamus/pathology , Pituitary Neoplasms/physiopathology , Prefrontal Cortex/physiopathology , Adolescent , Adult , Brain Mapping , Facial Expression , Female , Humans , Magnetic Resonance Imaging , Male , Nerve Net/physiopathology , Recognition, Psychology/physiology , Young Adult
9.
J Pediatr ; 164(4): 876-881.e4, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24507865

ABSTRACT

OBJECTIVE: To test memory performance and executive functions in patients with childhood craniopharyngioma and hypothalamic involvement. STUDY DESIGN: Using standardized neuropsychological tests, we compared cognitive performance in a group of 15 patients with childhood craniopharyngioma and known hypothalamic involvement and a group of 24 age- and intelligence-matched control subjects. In addition, we compared individual patients' results with normative data to detect abnormal performance in the clinically relevant range. Within the patient group, we further tested whether the grade of hypothalamic involvement had an impact on cognitive performance and quality of life. RESULTS: Relative to healthy controls, the patients demonstrated significantly lower performance scores in tests of memory and executive functioning. On the individual performance level, delayed recall performance was severely impaired in one-third of the patients. Compared with patients with low-grade hypothalamic involvement, those with high-grade hypothalamic involvement showed worse performance in executive functions and reduced functional capabilities for daily life actions, indicating lower quality of life. CONCLUSION: Our findings demonstrate that hypothalamic involvement is related to impairments in memory and executive functioning in patients with childhood craniopharyngioma and indicate that a high grade of hypothalamic involvement is related to worse outcomes.


Subject(s)
Craniopharyngioma/physiopathology , Hypothalamic Neoplasms/physiopathology , Neoplasms, Multiple Primary/physiopathology , Pituitary Neoplasms/physiopathology , Adolescent , Child , Female , Humans , Male , Neuropsychological Tests
10.
Metabolism ; 62(2): 226-34, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23040414

ABSTRACT

OBJECTIVE: Brain-derived neurotrophic factor (BDNF) is a regulator of energy homeostasis and food intake through hypothalamic signaling. Currently, data regarding BDNF in children with obesity are lacking. We evaluated serum BDNF concentrations in obese children, both before and after lifestyle intervention, in reference to those of lean children. METHODS: A total of 90 (24 normal weight; 66 obese) children were studied utilizing a cross-sectional clinical outpatient study design. In addition, longitudinal data analysis was performed in 30 obese children participating in a lifestyle intervention for one year. RESULTS: Fasting serum BDNF concentrations were higher in obese vs. normal weight children (BDNF 20.3±1.0 vs. 12.5±1.7 ng/mL, respectively, mean±SEM, p<0.001) and correlated significantly to BMI standard deviation score (r=0.426, p<0.001), and leptin (r=0.414, p<0.01). BDNF concentrations were not regulated in response to food, 60 min after ingestion of a liquid test meal. After one year lifestyle intervention, delta BDNF correlated significantly to delta leptin (r=0.475, p<0.01), but not to changes of insulin resistance index HOMA-IR, systolic and diastolic blood pressure, HDL, LDL, and triglycerides. In a multiple stepwise linear regression adjusted for pubertal stage, age, sex, and BMI, delta BDNF correlated significantly (p<0.05) to delta leptin and delta triceps skinfold and in tendency to delta subscapularis skinfold thickness (p=0.050). CONCLUSIONS: Our results in children do not indicate a significant relationship between BDNF and insulin resistance or cardiovascular risk factors. However, the correlation between changes of BDNF and changes of leptin suggests a relationship between BDNF and fat mass.


Subject(s)
Brain-Derived Neurotrophic Factor/blood , Insulin Resistance/physiology , Leptin/blood , Obesity/blood , Adolescent , Body Mass Index , Child , Cholesterol/blood , Cross-Sectional Studies , Female , Humans , Life Style , Longitudinal Studies , Male , Triglycerides/blood , Weight Loss/physiology
11.
J Clin Endocrinol Metab ; 97(11): 3935-43, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22969141

ABSTRACT

CONTEXT: Craniopharyngioma (CP), Rathke's cyst (RC), and xanthogranuloma (XG) are closely related rare sellar masses. Treatment strategies in children lack consensus. OBJECTIVE: Our objective was to study clinical manifestations and treatment-related outcome in RC, XG, and CP patients. DESIGN: We conducted a multicenter surveillance trial. PATIENTS: Inclusion criteria were 1) histological diagnosis of CP, XG, or RC and 2) diagnosis at age of 18 yr or less. A total of 117 CP, 14 XG (2001-2006), and 14 RC (1996-2006) were recruited. MAIN OUTCOME: Overall survival (OS), event-free survival (EFS), and quality of life (QoL) were evaluated. RESULTS: The 5-yr OS rates were 1.00 ± 0.00 in RC and XG and 0.97 ± 0.02 in CP. The 5-yr EFS rates were 0.85 ± 0.10 in RC, 1.00 ± 0.00 in XG, and 0.50 ± 0.05 in CP. Surgical resection of XG results in complete remission without recurrence. Recurrences occur in RC (14%) and CR (59%) but can be efficiently treated by irradiation, reoperation, and/or intracystic treatment. Severe hypothalamic sequelae such as obesity and others affecting QoL are predominant in CP due to presurgical involvement (59%) and postsurgical lesions (44%) of posterior hypothalamic structures. Centers with lower neurosurgery patient load use more radical surgical approaches to treat CP, resulting in higher rates of obesity and reduced QoL. Despite 46% anterior hypothalamic involvement, severe obesity is not encountered in XG. CONCLUSIONS: Treatment of choice in XG and RC is radical surgery. In CP involving hypothalamic structures, less radical surgical approaches preserving hypothalamic integrity are recommended. Due to frequent relapses, regular imaging during follow-up is recommended for CP and RC. Treatment of patients with sellar masses should be confined to experienced multidisciplinary teams.


Subject(s)
Central Nervous System Cysts/mortality , Craniopharyngioma/mortality , Pituitary Neoplasms/mortality , Xanthogranuloma, Juvenile/mortality , Adolescent , Central Nervous System Cysts/pathology , Central Nervous System Cysts/therapy , Child , Child, Preschool , Craniopharyngioma/pathology , Craniopharyngioma/therapy , Epidemiological Monitoring , Female , Humans , Infant , Male , Pituitary Neoplasms/pathology , Pituitary Neoplasms/therapy , Prospective Studies , Quality of Life , Sella Turcica/pathology , Sella Turcica/surgery , Survival Rate , Treatment Outcome , Xanthogranuloma, Juvenile/pathology , Xanthogranuloma, Juvenile/therapy
12.
Eur J Endocrinol ; 165(1): 17-24, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21490122

ABSTRACT

BACKGROUND: Hypothalamic obesity has major impact on prognosis and quality of life (QoL) in childhood craniopharyngioma. PATIENTS AND METHODS: For this study, 120 patients were prospectively recruited during 2001 and 2007 and evaluated after 3 years of follow-up (KRANIOPHARYNGEOM 2000). Body mass index (BMI) and QoL at diagnosis and 36 months after diagnosis were analysed based on the reference assessment of tumour localisation and post-surgical hypothalamic lesions. Treatment was analysed based on the neurosurgical strategy of 50 participating neurosurgical centres, the centre size based on the patient load. RESULTS: BMI SDS at diagnosis was similar in patients with or without hypothalamic involvement. Surgical lesions of anterior and posterior hypothalamic areas were associated with higher increase in BMI SDS during 36 months post-diagnosis compared with patients without or only anterior lesion (+1.8 BMISD, P=0.033, +2.1 BMISD; P=0.011), negative impact on QoL in patients with posterior hypothalamic lesions. Surgical strategies varied among the 50 neurosurgical centres (three large-sized, 24 middle-sized and 23 small-sized centres). Patients treated in small-sized centres presented with a higher rate of hypothalamic involvement compared with those treated in the middle- and large-sized centres. Treatment in large-sized centres was less radical, and the rates of complete resection and hypothalamic surgical lesions were lower in large-sized centres than those of the middle- and small-sized centres. However, a multivariable analysis showed that pre-operative hypothalamic involvement was the only independent risk factor for severe obesity (P=0.002). CONCLUSIONS: Radical neurosurgical strategies leading to posterior hypothalamic lesions are not recommended due to the potential to exacerbate hypothalamic obesity and impaired QoL. Treatment should be confined to experienced multidisciplinary teams.


Subject(s)
Craniopharyngioma/complications , Obesity/etiology , Adolescent , Child , Child, Preschool , Craniopharyngioma/surgery , Female , Humans , Hypothalamus/surgery , Infant , Longitudinal Studies , Magnetic Resonance Imaging , Male , Pituitary Neoplasms/complications , Pituitary Neoplasms/surgery , Postoperative Period , Prospective Studies , Quality of Life
13.
Obesity (Silver Spring) ; 19(1): 36-42, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20379145

ABSTRACT

Patients with craniopharyngioma (CP), an embryological tumor located in the hypothalamic and/or pituitary region, often suffer from uncontrolled eating and severe obesity. We aimed to compare peripherally secreted hormones involved in controlling food intake in normal weight and obese children and adolescents with CP vs. controls. Plasma insulin, glucose, total ghrelin, and peptide-YY (PYY) levels were assessed under fasting conditions as well as 60 min after liquid mixed meal in four groups: Normal weight (n = 12) and obese (n = 15) CP patients, and 12 normal weight and 15 obese otherwise healthy BMI-, gender- and age-matched controls. Homeostasis model assessment of insulin resistance (HOMA(IR)), as well as quantitative insulin sensitivity check index (QUICKI) were calculated. Obese CP subjects had significantly higher HOMA(IR), higher baseline and postmeal insulin but lower ghrelin levels, weaker postmeal changes for PYY, and lower QUICKI compared to obese controls. QUICKI data from all CP patients correlated positively with ghrelin and PYY % postmeal changes (ghrelin: r = 0.38, P = 0.023; PYY r = 0.40, P = 0.017) and negatively with standard deviation score-BMI (SDS-BMI: r = -0.49, P = 0.002). Tumor growth of 87% obese and 58% of normal weight CP patients affected the hypothalamic area which was associated with higher SDS-BMI and weaker % postmeal ghrelin changes (P = 0.014) compared to CP patients without hypothalamic tumor involvement. Blunted postmeal ghrelin and PYY responses in obese CP subjects are likely due to their higher degree of insulin resistance and lower insulin sensitivity compared to matched obese controls. Thus, insulin resistance in CP patients seems to affect eating behavior by affecting meal responses of gut peptides.


Subject(s)
Craniopharyngioma/blood , Ghrelin/blood , Pituitary Neoplasms/blood , Adolescent , Adult , Aged , Aged, 80 and over , Blood Glucose/analysis , Blood Glucose/metabolism , Case-Control Studies , Child , Child, Preschool , Craniopharyngioma/metabolism , Eating/physiology , Female , Ghrelin/metabolism , Humans , Infant , Infant, Newborn , Male , Middle Aged , Pituitary Neoplasms/metabolism , Young Adult
14.
Horm Res Paediatr ; 73(3): 175-80, 2010.
Article in English | MEDLINE | ID: mdl-20197669

ABSTRACT

BACKGROUND: Controversies surround various treatment variables for patients with childhood craniopharyngioma such as growth hormone (GH) replacement, which some believe can exacerbate recurrence/progression. We prospectively assessed the risk of tumor recurrence/progression in survivors of childhood craniopharyngioma. METHODS: Multivariable analyses of risk factors (age at diagnosis, degree of resection, irradiation, GH treatment and gender) and descriptive analyses of overall survival (OS) and event-free survival (EFS) rates were performed in 117 patients, recruited prospectively and evaluated after 3 years of follow-up in the German, Austrian and Swiss multicenter trial KRANIOPHARYNGEOM 2000. RESULTS: We observed a 3-year OS of 0.97 and a 3-year EFS of 0.46, indicating high recurrence rates after complete resection (CR) (n = 47; 3-year-EFS: 0.64) and high progression rates after incomplete resection (IR) (n = 64; 3-year EFS: 0.31). The risk of an event decreased by 80% after CR compared to IR (hazard ratio = 0.20; p < 0.001). Irradiation had protective effects on EFS: irradiated patients had an 88% lower risk of recurrence/progression compared to patients without/before irradiation (hazard ratio = 0.12; p < 0.001). GH treatment had no impact on 3-year EFS rates. CONCLUSIONS: Tumor recurrences/progressions are frequent and occur early after initial treatment of childhood craniopharyngioma. A radical resection preserving the integrity of hypothalamic structures appears optimal at original diagnosis. Irradiation was efficient in preventing recurrences/progressions. GH treatment had no impact on the low 3-year EFS observed in our study. However, further conclusions on the influence of GH on recurrence rates have to be refined to long-term follow-up studies of patients with childhood craniopharyngioma.


Subject(s)
Craniopharyngioma/therapy , Pituitary Neoplasms/therapy , Adolescent , Child , Child, Preschool , Craniopharyngioma/drug therapy , Craniopharyngioma/radiotherapy , Craniopharyngioma/surgery , Disease Progression , Disease-Free Survival , Female , Follow-Up Studies , Human Growth Hormone/therapeutic use , Humans , Infant , Male , Multivariate Analysis , Neoplasm Recurrence, Local/prevention & control , Pituitary Neoplasms/drug therapy , Pituitary Neoplasms/radiotherapy , Pituitary Neoplasms/surgery , Prospective Studies , Risk Factors , Treatment Outcome
15.
Metabolism ; 59(2): 186-94, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19766264

ABSTRACT

Relationships of blood circulating melanocortins to childhood obesity are not well established. We evaluated serum alpha-melanocyte-stimulating hormone (alpha-MSH) in lean children and different study groups of childhood obesity. We examined serum alpha-MSH in 52 otherwise healthy children with childhood obesity (Ob; mean age, 11 years; 32 girls/20 boys), 27 normal-weight children of same age, 7 additional obese patients with reduced melanocortin-4 receptor function (MC4Rmut), and 22 patients with craniopharyngioma (CP). Fasting serum alpha-MSH and leptin were measured by radioimmunoassay. Serum alpha-MSH was also evaluated 1 hour after 500-kcal liquid meal (CP and Ob) and at the end of 1-year lifestyle intervention in 24 Ob patients. The alpha-MSH levels were similar in obese vs lean children but significantly lower in CP (P < .001) and significantly higher (P < .05) in MC4Rmut patients compared with Ob. One hour after liquid meal, alpha-MSH increased in patients with Ob but not with CP. After 1 year, alpha-MSH levels increased significantly in the successful weight reduction Ob subgroup despite unchanged cortisol levels. The alpha-MSH changes correlated to weight status changes (r = 0.67, P = .0003) but not to changes of cortisol, insulin, or homeostasis model assessment of insulin resistance index. Persistently low alpha-MSH levels in CP patients are suspected to be due to pituitary or hypothalamic damage. High peripheral levels in MC4Rmut carriers indicate up-regulation of alpha-MSH. Changes of weight status are associated with changes of peripheral alpha-MSH.


Subject(s)
Obesity/blood , alpha-MSH/blood , Adolescent , Body Mass Index , Body Weight , Child , Craniopharyngioma/blood , Female , Genotype , Humans , Hydrocortisone/blood , Insulin/blood , Insulin Resistance , Leptin/blood , Male , Mutation , Obesity/genetics , Obesity/therapy , Pituitary Neoplasms/blood , Receptor, Melanocortin, Type 4/genetics
16.
Pediatr Res ; 61(4): 496-501, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17515878

ABSTRACT

Severe obesity is a major problem in patients suffering from craniopharyngioma (CP), a benign tumor located in pituitary and hypothalamic regions. In this study, the hypothesis that hypothalamic damage leads to a reduction in overall sympathetic tone was tested. Catecholamines, as well as their metabolites homovanillic acid (HVA) and vanillylmandelic acid (VMA), markers of catecholamine turnover, were measured in morning voided urine of 109 patients participating in a German pediatric CP study, and their physical activity was analyzed using a questionnaire. HVA and VMA results were compared with age-matched HVA and VMA in urine of patients proven to not have a catecholamine-secreting tumor. Patients with the most severe obesity displayed the lowest urine HVA and VMA values. Patients with hypothalamic CP had 3.2-fold higher BMI values (p<0.0001), lower HVA (0.72-fold, p<0.001), and VMA (0.84-fold, p<0.01) values, and significantly lower activity scores than those without hypothalamic involvement, but their epinephrine- and norepinephrine/creatinine ratios were not significantly different, possibly due to low levels. The low HVA and VMA values suggest decreased sympathetic outflow contributing to reduced physical activity and severe obesity, especially in patients with a hypothalamic tumor. In further studies investigating treatment options for hypothalamic obesity, disturbed sympathetic tone should be considered.


Subject(s)
Craniopharyngioma/urine , Obesity/urine , Sympathetic Nervous System/metabolism , Adolescent , Adult , Dopamine/urine , Epinephrine/urine , Female , Homovanillic Acid/urine , Humans , Male , Norepinephrine/urine , Vanilmandelic Acid/urine
17.
J Pediatr Endocrinol Metab ; 19 Suppl 1: 423-9, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16700320

ABSTRACT

Prognosis in childhood craniopharyngioma survivors hinges upon late effects such as pituitary deficiency and obesity. Observations indicate that reduced physical activity and increased daytime sleepiness might be risk factors for obesity. We analyzed the degree of daytime sleepiness in 115 childhood craniopharyngioma patients (47% obese) using the Epworth Sleepiness Scale (ESS). Thirty-five (30%) displayed increased daytime sleepiness (ESS score > 10) of whom 14 were obese (26% of obese cohort). Polysomnography (PSG) and Multiple Sleep Latency Tests (MSLT) were conducted with 10 obese patients presenting increased daytime sleepiness, with only two craniopharyngioma patients revealing a sleep related breathing disorder. Four patients had repeated episodes of SOREM (sleep onset rapid eye movement), the classic PSG criterion for narcolepsy. Three patients displayed hypersomnia. All but one patient qualified as acutely obese. We speculate that secondary narcolepsy is an exacerbating condition of childhood craniopharyngioma obesity, supported by recent reports on orexin and narcolepsy which suggest hypothalamic failure in idiopathic narcolepsy.


Subject(s)
Craniopharyngioma/complications , Narcolepsy/etiology , Obesity/complications , Pituitary Neoplasms/complications , Adolescent , Adult , Body Mass Index , Central Nervous System Stimulants/therapeutic use , Child , Cohort Studies , Female , HLA Antigens/metabolism , Humans , Male , Narcolepsy/drug therapy , Polysomnography , Risk Factors , Sleep/physiology , Sleep Stages
19.
Cancer Causes Control ; 17(4): 583-9, 2006 May.
Article in English | MEDLINE | ID: mdl-16596314

ABSTRACT

Craniopharyngioma is a rare dysontogenetic benign tumor. Patients frequently suffer from endocrine deficiencies, sleep disturbances and obesity due to pituitary and hypothalamic lesions. A self-assessment daytime sleepiness questionnaire (German version of the Epworth Sleepiness Scale [ESS]) was used to evaluate 79 patients with childhood craniopharyngioma. Because hypothalamic lesions may explain daytime sleepiness in craniopharyngioma patients, salivary melatonin and cortisol concentrations were examined in severely obese (BMI>or=4SD) and non severely obese (BMI<4SD) craniopharyngioma patients (n=79), patients with hypothalamic pilocytic astrocytoma (n=19), and control subjects (n=30). Using a general linear model procedure analyzing the influence of BMI and tumor diagnosis on diurnal salivary melatonin we found that morning salivary melatonin levels were related to BMI (F test: p-value=0.004) and tumor diagnosis (F-test: p-value=0.032). Also for nighttime salivary melatonin levels significant relations with BMI (p-value in F-test: <0.001) and tumor diagnosis (p-value in F-test: 0.025) were detectable. Melatonin concentrations in saliva of craniopharyngioma patients collected at nighttime or in the morning showed a negative correlation (Spearman's rho: -0.42; p=0.001; Spearman's rho: -0.31; p=0.020) with the patient's ESS score. Severely obese craniopharyngioma patients and severely obese hypothalamic tumor patients had similar patterns of melatonin secretion. Differences in terms of diurnal salivary cortisol concentrations were not detectable when patient groups and controls were compared. As decreased nocturnal melatonin levels were associated with increased daytime sleepiness, BMI and hypothalamic tumor diagnosis, we initiated an experimental melatonin substitution in 10 adult obese patients (5f/5m) with childhood craniopharyngioma. In all 10 patients with childhood craniopharyngioma the degree of daytime sleepiness significantly improved based on activity diaries, ESS, self assessment questionnaires and actimetry. We speculate that hypothalamic lesions might be responsible for both obesity and daytime sleepiness. As first experiences with experimental melatonin substitution were promising, further randomized double-blinded studies on the beneficial effects of melatonin substitution on daytime sleepiness and weight control in these patients are warranted.


Subject(s)
Craniopharyngioma/metabolism , Melatonin/metabolism , Melatonin/therapeutic use , Obesity/metabolism , Pituitary Neoplasms/metabolism , Sleep Stages , Adolescent , Adult , Astrocytoma/complications , Astrocytoma/metabolism , Child , Child, Preschool , Circadian Rhythm , Craniopharyngioma/complications , Disorders of Excessive Somnolence/complications , Disorders of Excessive Somnolence/drug therapy , Female , Humans , Hydrocortisone/blood , Hypothalamic Neoplasms/metabolism , Male , Obesity/complications , Pituitary Neoplasms/complications , Saliva/chemistry , Sleep Stages/drug effects
20.
Childs Nerv Syst ; 21(11): 975-80, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16075216

ABSTRACT

OBJECTS: We longitudinally analysed functional capacity and quality of life (QoL) in 102 patients with a childhood craniopharyngioma during follow-up. METHODS: The Fertigkeitenskala Münster-Heidelberg (FMH) ability scale was used for QoL assessment. Multivariate analysis evaluated FMH scores at various time points, examining hypothalamus involvement (HI), body mass index (BMI), degree of resection, tumour progression, relapse and irradiation. RESULTS AND CONCLUSION: Patients without HI (n=60) self-assessed higher QoL at baseline (p=0.001) and follow-up (p<0.001) than patients with HI (n=42). Only patients without HI evaluated at baseline >12 months after diagnosis showed longitudinal improvement in FMH scores (p=0.040). Rates of incomplete resection and irradiation were higher (p=0.070 and p=0.002 respectively) in patients with HI. In multivariate analysis, only HI, tumour relapse, progression, baseline FMH score, and time between diagnosis and baseline evaluation had independent impact on QoL. HI, tumour progression, and relapse had long-term QoL affects-most notably, severe obesity.


Subject(s)
Craniopharyngioma/psychology , Hypophysectomy/psychology , Pituitary Neoplasms/psychology , Quality of Life/psychology , Survivors/psychology , Activities of Daily Living/classification , Activities of Daily Living/psychology , Adolescent , Adult , Body Mass Index , Child , Child, Preschool , Combined Modality Therapy , Craniopharyngioma/radiotherapy , Craniopharyngioma/surgery , Disease Progression , Female , Follow-Up Studies , Humans , Hypothalamus/pathology , Infant , Longitudinal Studies , Male , Neoplasm Invasiveness , Neoplasm Recurrence, Local/psychology , Obesity/psychology , Pituitary Irradiation , Pituitary Neoplasms/radiotherapy , Pituitary Neoplasms/surgery , Postoperative Complications/psychology , Psychometrics/statistics & numerical data , Radiotherapy, Adjuvant , Reproducibility of Results , Self-Assessment , Sickness Impact Profile
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