[Accessible and total molecular surfaces of unsmooth protein globules]. / dostupnaia i obschchaia molekuliarnaia poverkhnosti negladkikh belkovykh globul.

A method for the calculation of accessible and total molecular surfaces of protein globules is proposed. It is based on consideration of structuring of protein globule surface. As a whole, the protein globule is approximated by ellipsoids. By computer imitations, the areas of accessible and total molecular surfaces as well as the volumes for 58 globular proteins were calculated. The calculation showed that the best agreement between the calculated areas and volumes and the experimental results is obtained at the radii of protein surface structures of 1-2 A.

[Distribution of ionizing radiation absorption in microstructures of a biosystem]. / Raspredelenie pogloshcheniia énergii ioniziruiushchego izlucheniia po mikrostrukturam biosistem.

The method of determination of the gamma-radiation absorption distribution in microstructures of a biosystem is proposed. The absorption capacity and energy absorption distribution of some biomolecules, mass coefficients and optical density of blood and thyroid gland are calculated. Influence of the pollution of biosystem microstructures (liver, blood, thyroid, gland) by hard elements on the energy absorption distribution is demonstrated.

[Phagocytosis in calves and its dynamics after experimental treatment]. / Fagocytóza tel'at a jej dynamika po experimentálnom zásahu.

We evaluated the phagocytic capacity of blood neutrophils and monocytes in calves after nonspecific stimulations of the organisms by colloidal charcoal and concentrated blood derivative administered alone, or enriched by immunomodulator (levamizol). Each of the above-mentioned preparations could be characterized by a specific response of the organism with respect to the phagocytic capacity. After administration of colloidal charcoal, both the phagocytic activity (percent of phagocytizing cells) and the particle-ingesting ability, i.e. phagocytic index, which showed two-phase changes in relation to the administration, were simulated. An increase in the phagocytic index was retained until the end of observation (four weeks), but the phagocytic activity (percent of phagocytizing cells) dropped to the level before administration as soon as in 24 hours. The phagocytic activity was also positively stimulated by the concentrated blood derivative administered alone, and the particle-ingesting ability was not influenced very much. The concentrated blood derivative with immunomodulator could be characterized by a fast increase in the phagocytic activity, accompanied by a successive short-time rise of the phagocytic index. A joint feature of all three preparations was a simultaneous increase in the percent of potential phagocytes and in the values of phagocytic activity. In none of the cases was, however, the increase in phagocytic activity accompanied by a rise in the phagocytic index.