ABSTRACT
OBJECTIVE: To detect the diagnostic accuracy of rapid antibody detection test using IgM immunochromatography for scrub typhus in children. METHODS: This cross-sectional study enrolled children aged 2 months-18 years hospitalized over a period of 18 months with undifferentiated fever of duration five days or more. The blood samples were subjected to serological tests like Weil-Felix, Scrub IgM ELISA, immunofluroscence assay (IFA) and rapid diagnostic test (IgM Immunochromatography). Diagnostic accuracy was measured against IFA as the gold standard. RESULTS: A total of 90 children were included in the study, among which 43 children were positive for gold standard test IFA. Rapid diagnostic test showed sensitivity of 88.3%, specificity of 89.3%, positive predictive value of 88.3% and negative predictive value of 89.3%. The sensitivity, specificity, PPV and NPV of Weil-Felix test was 39.5%, 84.2%, 58.6 and 71.1%, respectively and of IgM ELISA was 93%, 89.3%,88.8% and 93.3%, respectively. CONCLUSION: IgM immunochromatography had good diagnostic accuracy for scrub typhus in children with acute undifferentiated fever.
Subject(s)
Scrub Typhus , Child , Humans , Scrub Typhus/diagnosis , Cross-Sectional Studies , Sensitivity and Specificity , Enzyme-Linked Immunosorbent Assay/methods , Fever , Immunoglobulin M , Antibodies, BacterialABSTRACT
An ethnopharmacological approach for biosynthesis of gold nanoparticles is being demonstrated using seed coat of Cajanus cajan. Medicinal value of capping molecule investigated for anticancer activity and results disclose its greater potential. The active principle of the seed coat [3-butoxy-2-hydroxypropyl 2-(2,4-dihydroxyphenyl) acetate] is elucidated. Rapid one-step synthesis yields highly stable, monodisperse (spherical) gold nanoparticles in the size ranging from 9 to 41 nm. Anticancer activity has been studied using liver cancer cells and cytotoxic mechanism has been evaluated using MTT, Annexin-V/PI Double-Staining Assay, Cell cycle, Comet assay and Flow cytometric analysis for apoptosis. The present investigation will open up a new possibility of functionalizing gold nanoparticles for apoptosis studies in liver cancer cells.
Subject(s)
Gold/chemistry , Metal Nanoparticles/chemistry , Apoptosis/drug effects , Hep G2 Cells , HumansABSTRACT
As the nano revolution unfolds, it is imperative to integrate nanoscience and medicine. The secret gleaned from nature have led to the generation of biogenic technologies for the fabrication of advanced nanomaterials. Present investigation discloses the gold nanoparticles biosynthesizing capability of the flower of pharmacologically important tree Couroupita guianensis. Rapid, cost-effective, one-step process of synthesis has been achieved. Newly genre gold nanoparticles were characterized by involving UV-vis spectroscopy, FTIR, XRD, SEM, and TEM analysis. Interestingly, as a result of extensive screening on the application of newly synthesized gold nanoparticles their anticancer potential has been discovered using MTT assay, DNA fragmentation, apoptosis by DAPI staining, and comet assay for DNA damage.
