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1.
Integr Cancer Ther ; 19: 1534735420918945, 2020.
Article in English | MEDLINE | ID: mdl-32493139

ABSTRACT

Naturally occurring flavonoids, such as acacetin and pinostrobin, disrupt a wide range of processes during tumor progression, such as cell proliferation, apoptosis, and angiogenesis. Although the antiproliferative and antiapoptotic effects of acacetin and pinostrobin have been studied using various cell lines, relatively little is known about the effects of acacetin and pinostrobin on cancer cell migration and metastasis. For instance, it is unclear whether acacetin or pinostrobin have any effect on breast cancer cell migration or adhesion. In this study, we assessed the effects of acacetin and pinostrobin on malignant MDA-MB-231 and T47D breast epithelial cells and non-tumorigenic MCF10A breast epithelial cells. Our results demonstrate that both acacetin and pinostrobin selectively inhibit the migration of both MDA-MB-231 and T47D cells in a dose-dependent manner while exhibiting blunted effects on MCF10A cells. Interestingly, neither compound had an effect on cell proliferation in any of the 3 cell lines. Furthermore, both acacetin and pinostrobin inhibit MDA-MB-231 and T47D cell adhesion, cell spreading, and focal adhesion formation, but have no significant effect on MCF10A cells. Collectively, these results suggest that both acacetin and pinostrobin selectively inhibit malignant breast epithelial cell migration through attenuation of cell adhesion and focal adhesion formation. These findings indicate that both acacetin and pinostrobin may serve as potential therapeutic options to target breast tumor cell migration during late-stage tumor progression.


Subject(s)
Breast Neoplasms , Cell Movement , Focal Adhesions , Cell Adhesion , Cell Line, Tumor , Cell Proliferation , Epithelial Cells , Female , Flavanones/pharmacology , Flavones/pharmacology , Humans
2.
Int J Breast Cancer ; 2017: 9619734, 2017.
Article in English | MEDLINE | ID: mdl-28182100

ABSTRACT

Interactions between integrin-mediated adhesions and the extracellular matrix (ECM) are important regulators of cell migration and spreading. However, mechanisms by which extracellular ligands regulate cell migration and spreading in response to changes in substratum concentration are not well understood. Semaphorin 3A (Sema3A) has been shown to inhibit cell motility and alter integrin signaling in various cell types. We propose that Sema3A alters focal adhesions to modulate breast carcinoma cell migration and spreading on substrata coated with different concentrations of ECM. We demonstrate that Sema3A inhibits MDA-MB-231 cell migration and spreading on substrata coated with high concentrations of collagen and fibronectin but enhances migration and spreading at lower concentrations of collagen and fibronectin. Sema3A increases focal adhesion kinase phosphorylation at tyrosine 397 (pFAK397) at focal adhesions on all substratum concentrations of collagen and fibronectin but decreased pFAK397 levels on laminin. Rho-associated protein kinase (ROCK) inhibition blocks the Sema3A-mediated effects on cell migration, spreading, and pFAK397 at focal adhesions when cultured on all concentrations of collagen. These results suggest that Sema3A shifts the optimal level of cell-matrix adhesions to a nonoptimal ECM coating concentration, in particular collagen, to yield maximal cell migration and spreading that may be mediated through a ROCK-dependent mechanism.

3.
Crit Rev Eukaryot Gene Expr ; 23(2): 139-57, 2013.
Article in English | MEDLINE | ID: mdl-23582036

ABSTRACT

Cell transformation and tumor progression involve a common set of acquired capabilities, including increased proliferation, failure of cell death, self-sufficiency in growth, angiogenesis, and tumor cell invasion and metastasis. The stromal environment consists of many cell types and various extracellular matrix (ECM) proteins that support normal tissue maintenance and which have been implicated in tumor progression. Both the chemical and mechanical properties of the ECM have been shown to influence normal and malignant cell behavior. For instance, mesenchymal stem cells differentiate into specific lineages that are dependent on matrix stiffness, while tumor cells undergo changes in cell behavior and gene expression in response to matrix stiffness. ECM remodeling is implicated in tumor progression and can result in increased deposition of stromal ECM, enhanced contraction of ECM fibrils, and altered collagen alignment and ECM stiffness. Tumor cells respond to changes in ECM remodeling through altered intracellular signaling and cell cycle control that lead to enhanced proliferation, loss of normal tissue architecture, and local tumor cell migration and invasion. This review focuses on the bi-directional interplay between the mechanical properties of the ECM and integrin-mediated signal transduction events in an effort to elucidate cell behaviors during tumor progression.


Subject(s)
Breast Neoplasms/metabolism , Extracellular Matrix/metabolism , Integrins/metabolism , Animals , Breast Neoplasms/pathology , Cell Cycle/physiology , Cell Movement , Collagen/metabolism , Disease Progression , Extracellular Matrix/pathology , Extracellular Matrix Proteins/metabolism , Female , Humans , Matrix Metalloproteinases/metabolism , Signal Transduction , rho GTP-Binding Proteins/metabolism
4.
Mol Biol Cell ; 20(14): 3224-38, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19458194

ABSTRACT

The physical properties of the extracellular matrix (ECM) regulate the behavior of several cell types; yet, mechanisms by which cells recognize and respond to changes in these properties are not clear. For example, breast epithelial cells undergo ductal morphogenesis only when cultured in a compliant collagen matrix, but not when the tension of the matrix is increased by loading collagen gels or by increasing collagen density. We report that the actin-binding protein filamin A (FLNa) is necessary for cells to contract collagen gels, and pull on collagen fibrils, which leads to collagen remodeling and morphogenesis in compliant, low-density gels. In stiffer, high-density gels, cells are not able to contract and remodel the matrix, and morphogenesis does not occur. However, increased FLNa-beta1 integrin interactions rescue gel contraction and remodeling in high-density gels, resulting in branching morphogenesis. These results suggest morphogenesis can be "tuned" by the balance between cell-generated contractility and opposing matrix stiffness. Our findings support a role for FLNa-beta1 integrin as a mechanosensitive complex that bidirectionally senses the tension of the matrix and, in turn, regulates cellular contractility and response to this matrix tension.


Subject(s)
Contractile Proteins/metabolism , Epithelial Cells/metabolism , Extracellular Matrix/metabolism , Integrin beta1/metabolism , Microfilament Proteins/metabolism , Animals , Biomechanical Phenomena , Cell Line, Tumor , Collagen/metabolism , Filamins , Gels/metabolism , Humans , Mice , Morphogenesis , Myosin Light Chains/metabolism , Phosphorylation , Protein Binding
5.
Methods Enzymol ; 426: 27-45, 2007.
Article in English | MEDLINE | ID: mdl-17697878

ABSTRACT

There has been much recent interest in working with cells cultured in three-dimensional (3D) matrices to better model the properties of the extracellular matrix environment found in vivo. However, working within 3D matrices adds several difficulties to experiments that have become routine in two-dimensional (2D) culture systems. Biochemical approaches are made difficult by the presence of milligram quantities of matrix protein, while cell biology approaches are more difficult to assess and image. Moreover, 3D imaging adds complexity to fluorescence studies, including the inherent challenge of a 3D volume as opposed to a 2D image, increased depths of field, and problems of light scatter. The purpose of this chapter is to provide a few overall strategies for working within 3D culture systems, focusing on biochemical and molecular imaging approaches.


Subject(s)
Imaging, Three-Dimensional , Integrins/physiology , Signal Transduction , Animals , Cell Culture Techniques , Cells, Cultured , Humans
6.
J Neurobiol ; 66(2): 103-14, 2006 Feb 05.
Article in English | MEDLINE | ID: mdl-16215999

ABSTRACT

Rho family GTPases have important roles in mediating the effects of guidance cues and growth factors on the motility of neuronal growth cones. We previously showed that the neurotrophin BDNF regulates filopodial dynamics on growth cones of retinal ganglion cell axons through activation of the actin regulatory proteins ADF and cofilin by inhibiting a RhoA-dependent pathway that phosphorylates (inactivates) ADF/cofilin. The GTPase Cdc42 has also been implicated in mediating the effects of positive guidance cues. In this article we investigated whether Cdc42 is involved in the effects of BDNF on filopodial dynamics. BDNF treatment increases Cdc42 activity in retinal neurons, and neuronal incorporation of constitutively active Cdc42 mimics the increases in filopodial number and length. Furthermore, constitutively active and dominant negative Cdc42 decreased and increased, respectively, the activity of RhoA in retinal growth cones, indicating crosstalk between these GTPases in retinal growth cones. Constitutively active Cdc42 mimicked the activation of ADF/cofilin that resulted from BDNF treatment, while dominant negative Cdc42 blocked the effects of BDNF on filopodia and ADF/cofilin. The inability of dominant negative Cdc42 to block ADF/cofilin activation and stimulation of filopodial dynamics by the ROCK inhibitor Y-27632 indicate interaction between Cdc42 and RhoA occurs upstream of ROCK. Our results demonstrate crosstalk occurs between GTPases in mediating the effects of BDNF on growth cone motility, and Cdc42 activity can promote actin dynamics via activation of ADF/cofilin.


Subject(s)
Actin Depolymerizing Factors/metabolism , Brain-Derived Neurotrophic Factor/metabolism , Growth Cones/metabolism , Pseudopodia/metabolism , Retina/metabolism , cdc42 GTP-Binding Protein/metabolism , Animals , Cells, Cultured , Chick Embryo , Enzyme Activation/physiology , Fluorescent Antibody Technique , Image Processing, Computer-Assisted
7.
J Neurosci ; 24(47): 10741-9, 2004 Nov 24.
Article in English | MEDLINE | ID: mdl-15564592

ABSTRACT

The molecular mechanisms by which neurotrophins regulate growth cone motility are not well understood. This study investigated the signaling involved in transducing BDNF-induced increases of filopodial dynamics. Our results indicate that BDNF regulates filopodial length and number through a Rho kinase-dependent mechanism. Additionally, actin depolymerizing factor (ADF)/cofilin activity is necessary and sufficient to transduce the effects of BDNF. Our data indicate that activation of ADF/cofilin mimics the effects of BDNF on filopodial dynamics, whereas ADF/cofilin inactivity blocks the effects of BDNF. Furthermore, BDNF promotes the activation of ADF/cofilin by reducing the phosphorylation of ADF/cofilin. Although inhibition of myosin II also enhances filopodial length, our results indicate that BDNF signaling is independent of myosin II activity and that the two pathways result in additive effects on filopodial length. Thus, filopodial extension is regulated by at least two independent mechanisms. The BDNF-dependent pathway works via regulation of ADF/cofilin, independently of myosin II activity.


Subject(s)
Brain-Derived Neurotrophic Factor/physiology , Growth Cones/physiology , Microfilament Proteins/physiology , Pseudopodia/physiology , Retina/ultrastructure , 14-3-3 Proteins/physiology , Actin Depolymerizing Factors , Animals , Brain-Derived Neurotrophic Factor/antagonists & inhibitors , Chick Embryo , Destrin , Growth Cones/ultrastructure , Heterocyclic Compounds, 4 or More Rings/pharmacology , Intracellular Signaling Peptides and Proteins , Microfilament Proteins/metabolism , Myosin Type II/antagonists & inhibitors , Myosin Type II/physiology , Phosphorylation , Protein Serine-Threonine Kinases/antagonists & inhibitors , Protein Serine-Threonine Kinases/physiology , Signal Transduction/physiology , Tissue Culture Techniques , rho-Associated Kinases
8.
J Neurosci ; 24(18): 4363-72, 2004 May 05.
Article in English | MEDLINE | ID: mdl-15128850

ABSTRACT

The mechanisms by which neurotrophins regulate growth cone motility are unclear. We investigated the role of the p75 neurotrophin receptor (p75NTR) in mediating neurotrophin-induced increases in filopodial length. Our data demonstrate that neurotrophin binding to p75NTR is necessary and sufficient to regulate filopodial dynamics. Furthermore, retinal and dorsal root ganglion growth cones from p75 mutant mice are insensitive to neurotrophins but display enhanced filopodial lengths comparable with neurotrophin-treated wild-type growth cones. This suggests unoccupied p75NTR negatively regulates filopodia length. Furthermore, p75NTR regulates RhoA activity to mediate filopodial dynamics. Constitutively active RhoA blocks neurotrophin-induced increases in filopodial length, whereas inhibition of RhoA enhances filopodial lengths, similar to neurotrophin treatment. BDNF treatment of retinal neurons results in reduced RhoA activity. Furthermore, p75 mutant neurons display reduced levels of activated RhoA compared with wild-type counterparts, consistent with the enhanced filopodial lengths observed on mutant growth cones. These observations suggest that neurotrophins regulate filopodial dynamics by depressing the activation of RhoA that occurs through p75NTR signaling.


Subject(s)
Growth Cones/physiology , Pseudopodia/physiology , Receptors, Nerve Growth Factor/physiology , Signal Transduction/physiology , rhoA GTP-Binding Protein/metabolism , Animals , Brain-Derived Neurotrophic Factor/pharmacology , Chick Embryo , Dose-Response Relationship, Drug , Enzyme Activation/physiology , Enzyme Inhibitors/pharmacology , Ganglia, Spinal/cytology , Ganglia, Spinal/embryology , Growth Cones/drug effects , Growth Cones/enzymology , Mice , Mice, Mutant Strains , Nerve Growth Factors/metabolism , Nerve Growth Factors/pharmacology , Neurons/drug effects , Neurons/metabolism , Neurons/ultrastructure , Pseudopodia/drug effects , Pseudopodia/enzymology , Receptor, Nerve Growth Factor , Receptors, Nerve Growth Factor/genetics , Receptors, Nerve Growth Factor/metabolism , Retina/cytology , Retina/embryology , rhoA GTP-Binding Protein/antagonists & inhibitors , rhoA GTP-Binding Protein/genetics
9.
Anat Sci Int ; 79(4): 173-83, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15633455

ABSTRACT

The regulation of filopodial dynamics by neurotrophins and other guidance cues plays an integral role in growth cone pathfinding. Filopodia are F-actin-based structures that explore the local environment, generate forces and play a role in growth cone translocation. Here, we review recent research showing that the actin-depolymerizing factor (ADF)/cofilin family of proteins mediates changes in the length and number of growth cone filopodia in response to brain-derived neurotrophic factor (BDNF). Although inhibition of myosin contractility also causes filopodial elongation, the elongation in response to BDNF does not occur through a myosin-dependent pathway. Active ADF/cofilin increases the rate of cycling between the monomer and polymer pools and is critical for the BDNF-induced changes. Thus, we discuss potential mechanisms by which ADF/cofilin may affect filopodial initiation and length change via its effects on F-actin dynamics in light of past research on actin and myosin function in growth cones.


Subject(s)
Growth Cones/metabolism , Microfilament Proteins/metabolism , Nerve Tissue Proteins/metabolism , Actin Depolymerizing Factors , Animals , Cell Line, Tumor , Destrin , Growth Cones/ultrastructure , Humans , Mice , Microfilament Proteins/ultrastructure , Nerve Tissue Proteins/ultrastructure
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