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1.
Sci Rep ; 14(1): 2061, 2024 01 24.
Article in English | MEDLINE | ID: mdl-38267530

ABSTRACT

Huntington's disease (HD) is a progressive neurodegenerative disorder caused by CAG trinucleotide repeat expansions in exon 1 of the HTT gene. In addition to germline CAG expansions, somatic repeat expansions in neurons also contribute to HD pathogenesis. The DNA mismatch repair gene, MSH3, identified as a genetic modifier of HD onset and progression, promotes somatic CAG expansions, and thus presents a potential therapeutic target. However, what extent of MSH3 protein reduction is needed to attenuate somatic CAG expansions and elicit therapeutic benefits in HD disease models is less clear. In our study, we employed potent di-siRNAs to silence mouse Msh3 mRNA expression in a dose-dependent manner in HdhQ111/+ mice and correlated somatic Htt CAG instability with MSH3 protein levels from simultaneously isolated DNA and protein after siRNA treatment. Our results reveal a linear correlation with a proportionality constant of ~ 1 between the prevention of somatic Htt CAG expansions and MSH3 protein expression in vivo, supporting MSH3 as a rate-limiting step in somatic expansions. Intriguingly, despite a 75% reduction in MSH3 protein levels, striatal nuclear HTT aggregates remained unchanged. We also note that evidence for nuclear Msh3 mRNA that is inaccessible to RNA interference was found, and that MSH6 protein in the striatum was upregulated following MSH3 knockdown in HdhQ111/+ mice. These results provide important clues to address critical questions for the development of therapeutic molecules targeting MSH3 as a potential therapeutic target for HD.


Subject(s)
Corpus Striatum , Huntington Disease , Animals , Mice , Exons , Huntington Disease/genetics , RNA Interference , RNA, Messenger , RNA, Small Interfering/genetics
2.
Genome Med ; 16(1): 13, 2024 01 16.
Article in English | MEDLINE | ID: mdl-38229148

ABSTRACT

BACKGROUND: Sudden unexpected death in children is a tragic event. Understanding the genetics of sudden death in the young (SDY) enables family counseling and cascade screening. The objective of this study was to characterize genetic variation in an SDY cohort using whole genome sequencing. METHODS: The SDY Case Registry is a National Institutes of Health/Centers for Disease Control and Prevention surveillance effort to discern the prevalence, causes, and risk factors for SDY. The SDY Case Registry prospectively collected clinical data and DNA biospecimens from SDY cases < 20 years of age. SDY cases were collected from medical examiner and coroner offices spanning 13 US jurisdictions from 2015 to 2019. The cohort included 211 children (median age 0.33 year; range 0-20 years), determined to have died suddenly and unexpectedly and from whom DNA biospecimens for DNA extractions and next-of-kin consent were ascertained. A control cohort consisted of 211 randomly sampled, sex- and ancestry-matched individuals from the 1000 Genomes Project. Genetic variation was evaluated in epilepsy, cardiomyopathy, and arrhythmia genes in the SDY and control cohorts. American College of Medical Genetics/Genomics guidelines were used to classify variants as pathogenic or likely pathogenic. Additionally, pathogenic and likely pathogenic genetic variation was identified using a Bayesian-based artificial intelligence (AI) tool. RESULTS: The SDY cohort was 43% European, 29% African, 3% Asian, 16% Hispanic, and 9% with mixed ancestries and 39% female. Six percent of the cohort was found to harbor a pathogenic or likely pathogenic genetic variant in an epilepsy, cardiomyopathy, or arrhythmia gene. The genomes of SDY cases, but not controls, were enriched for rare, potentially damaging variants in epilepsy, cardiomyopathy, and arrhythmia-related genes. A greater number of rare epilepsy genetic variants correlated with younger age at death. CONCLUSIONS: While damaging cardiomyopathy and arrhythmia genes are recognized contributors to SDY, we also observed an enrichment in epilepsy-related genes in the SDY cohort and a correlation between rare epilepsy variation and younger age at death. These findings emphasize the importance of considering epilepsy genes when evaluating SDY.


Subject(s)
Cardiomyopathies , Epilepsy , Child , Humans , Female , Infant , Male , Death, Sudden, Cardiac/etiology , Artificial Intelligence , Bayes Theorem , Arrhythmias, Cardiac/complications , Arrhythmias, Cardiac/genetics , Cardiomyopathies/genetics , Cardiomyopathies/complications , Epilepsy/genetics , DNA , Genetic Testing
3.
medRxiv ; 2023 Mar 29.
Article in English | MEDLINE | ID: mdl-37034657

ABSTRACT

Background: Sudden unexpected death in children is a tragic event. Understanding the genetics of sudden death in the young (SDY) enables family counseling and cascade screening. The objective of this study was to characterize genetic variation in an SDY cohort using whole genome sequencing. Methods: The SDY Case Registry is a National Institutes of Health/Centers for Disease Control surveillance effort to discern the prevalence, causes, and risk factors for SDY. The SDY Case Registry prospectively collected clinical data and DNA biospecimens from SDY cases <20 years of age. SDY cases were collected from medical examiner and coroner offices spanning 13 US jurisdictions from 2015-2019. The cohort included 211 children (mean age 1 year; range 0-20 years), determined to have died suddenly and unexpectedly and in whom DNA biospecimens and next-of-kin consent were ascertained. A control cohort consisted of 211 randomly sampled, sex-and ancestry-matched individuals from the 1000 Genomes Project. Genetic variation was evaluated in epilepsy, cardiomyopathy and arrhythmia genes in the SDY and control cohorts. American College of Medical Genetics/Genomics guidelines were used to classify variants as pathogenic or likely pathogenic. Additionally, genetic variation predicted to be damaging was identified using a Bayesian-based artificial intelligence (AI) tool. Results: The SDY cohort was 42% European, 30% African, 17% Hispanic, and 11% with mixed ancestries, and 39% female. Six percent of the cohort was found to harbor a pathogenic or likely pathogenic genetic variant in an epilepsy, cardiomyopathy or arrhythmia gene. The genomes of SDY cases, but not controls, were enriched for rare, damaging variants in epilepsy, cardiomyopathy and arrhythmia-related genes. A greater number of rare epilepsy genetic variants correlated with younger age at death. Conclusions: While damaging cardiomyopathy and arrhythmia genes are recognized contributors to SDY, we also observed an enrichment in epilepsy-related genes in the SDY cohort, and a correlation between rare epilepsy variation and younger age at death. These findings emphasize the importance of considering epilepsy genes when evaluating SDY.

4.
Pediatr Crit Care Med ; 21(9): e827-e833, 2020 09.
Article in English | MEDLINE | ID: mdl-32701748

ABSTRACT

OBJECTIVES: Neonatal cardiac surgery for congenital cardiac defects is associated with significant morbidity and mortality, and there is a need for early identification of patients at highest risk of adverse outcomes. Because vascular endothelial injury mediates damage across organ systems, we measured serum biomarkers of endothelial injury in neonates following cardiopulmonary bypass and examined their associations with short-term outcomes. DESIGN: Prospective cohort study. SETTING: Pediatric cardiac ICU. PATIENTS: Thirty neonates less than 2 weeks old undergoing repair of congenital cardiac defects with cardiopulmonary bypass. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Biomarkers of endothelial integrity, angiopoietin-1 and angiopoietin-2, were measured preoperatively and at 24 hours postoperatively. A composite adverse outcome was defined as any of the following: stroke, need for renal replacement therapy, extracorporeal membrane oxygenation support, cardiac arrest, or death. Associations of biomarkers with adverse outcomes were examined using Wilcoxon rank-sum test. There was an increase in angiopoietin-2 from preoperatively to 24 hours postoperatively (p < 0.0001) and a decrease in angiopoietin-1 from preoperatively to 24 hours postoperatively (p < 0.0001). Patients with greater rise in angiopoietin-2 from preoperatively to 24 hours postoperatively had greater risk of composite adverse outcome (p = 0.04). They had a trend toward higher Vasoactive-Inotropic Score (p = 0.06) and a higher prevalence of low cardiac output syndrome (p = 0.06). Twenty-four hour postoperative angiopoietin-2 level was associated with the composite adverse outcome (p = 0.03). The rise in angiopoietin-2 level from preoperatively to 24 hours postoperatively directly correlated with cardiopulmonary bypass duration (r = 0.47; p = 0.01). CONCLUSIONS: In neonatal cardiac surgery, longer duration of cardiopulmonary bypass is directly associated with greater endothelial injury as measured by increased serum levels of angiopoietin-2. Angiopoietin-2 levels 24 hours postoperatively were significantly associated with a composite adverse outcome. Postoperative angiopoietin-2 level may serve as an early indicator of patients in need of closer monitoring and protective intervention. Further research into endothelial protective strategies is warranted.


Subject(s)
Cardiac Surgical Procedures , Heart Defects, Congenital , Angiopoietin-2 , Cardiac Surgical Procedures/adverse effects , Cardiopulmonary Bypass/adverse effects , Child , Heart Defects, Congenital/surgery , Humans , Infant, Newborn , Postoperative Complications/diagnosis , Postoperative Complications/epidemiology , Postoperative Complications/etiology , Prospective Studies
5.
PLoS One ; 14(8): e0220125, 2019.
Article in English | MEDLINE | ID: mdl-31408457

ABSTRACT

BIN1 is the most important risk locus for Late Onset Alzheimer's Disease (LOAD), after ApoE. BIN1 AD-associated SNPs correlate with Tau deposition as well as with brain atrophy. Furthermore, the level of neuronal-specific BIN1 isoform 1 protein is decreased in sporadic AD cases in parallel with neuronal loss, despite an overall increase in BIN1 total mRNA. To address the relationship between reduction of BIN1 and neuronal cell loss in the context of Tau pathology, we knocked-down endogenous murine Bin1 via stereotaxic injection of AAV-Bin1 shRNA in the hippocampus of mice expressing Tau P301S (PS19). We observed a statistically significant reduction in the number of neurons in the hippocampus of mice injected with AAV-Bin1 shRNA in comparison with mice injected with AAV control. To investigate whether neuronal loss is due to deletion of Bin1 selectively in neurons in presence Tau P301S, we bred Bin1flox/flox with Thy1-Cre and subsequently with PS19 mice. Mice lacking neuronal Bin1 and expressing Tau P301S showed increased mortality, without increased neuropathology, when compared to neuronal Bin1 and Tau P301S-expressing mice. The loss of Bin1 isoform 1 resulted in reduced excitability in primary neurons in vitro, reduced neuronal c-fos expression as well as in altered microglia transcriptome in vivo. Taken together, our data suggest that the contribution of genetic variation in BIN1 locus to AD risk could result from a cell-autonomous reduction of neuronal excitability due to Bin1 decrease, exacerbated by the presence of aggregated Tau, coupled with a non-cell autonomous microglia activation.


Subject(s)
Adaptor Proteins, Signal Transducing/physiology , Alzheimer Disease/pathology , Biomarkers/metabolism , Brain/physiopathology , Disease Models, Animal , Hippocampus/physiopathology , Nerve Tissue Proteins/physiology , Neurons/pathology , Tumor Suppressor Proteins/physiology , Alzheimer Disease/genetics , Alzheimer Disease/metabolism , Animals , Behavior, Animal , Brain/metabolism , Female , Gene Expression Profiling , Hippocampus/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Neurons/metabolism , Rats , tau Proteins/metabolism
6.
Cell ; 178(3): 511, 2019 07 25.
Article in English | MEDLINE | ID: mdl-31348881
7.
Cell ; 174(6): 1329-1331, 2018 09 06.
Article in English | MEDLINE | ID: mdl-30193103
8.
Cell ; 173(5): 1059-1061, 2018 05 17.
Article in English | MEDLINE | ID: mdl-29775586
9.
Cell ; 170(5): 817-819, 2017 08 24.
Article in English | MEDLINE | ID: mdl-28841412
10.
Development ; 142(17): 2876-87, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-26329598

ABSTRACT

Intricate layers of regulation determine the unique gene expression profiles of a given cell and, therefore, underlie the immense phenotypic diversity observed among cell types. Understanding the mechanisms that govern which genes are expressed and which genes are silenced is a fundamental focus in biology. The Polycomb and Trithorax group chromatin proteins play important roles promoting the stable and heritable repression and activation of gene expression, respectively. These proteins, which are conserved across metazoans, modulate post-translational modifications on histone tails and regulate nucleosomal structures. Here, we review recent advances that have shed light on the mechanisms by which these two classes of proteins act to maintain epigenetic memory and allow dynamic switches in gene expression during development.


Subject(s)
Chromosomal Proteins, Non-Histone/metabolism , Gene Expression Regulation, Developmental , Polycomb-Group Proteins/metabolism , Animals , Histones/metabolism , Humans , Protein Processing, Post-Translational , RNA, Long Noncoding/metabolism
11.
Congenit Heart Dis ; 10(3): 193-208, 2015.
Article in English | MEDLINE | ID: mdl-24720490

ABSTRACT

OBJECTIVE: Congenital cardiac defects represent the most common group of birth defects, affecting an estimated six per 1000 births. Genetic characterization of patients and families with cardiac defects has identified a number of genes required for heart development. Yet, despite the rapid pace of these advances, mutations affecting known genes still account for only a small fraction of congenital heart defects suggesting that many more genes and developmental mechanisms remain to be identified. DESIGN: In this study, we reviewed 1694 described cases of patients with cardiac defects who were determined to have a significant chromosomal imbalance (a deletion or duplication). The cases were collected from publicly available databases (DECIPHER, ISCA, and CHDWiki) and from recent publications. An additional 68 nonredundant cases were included from the University of Michigan. Cases with multiple chromosomal or whole chromosome defects (trisomy 13, 18, 21) were excluded, and cases with overlapping deletions and/or insertions were grouped to identify regions potentially involved in heart development. RESULTS: Seventy-nine chromosomal regions were identified in which 5 or more patients had overlapping imbalances. Regions of overlap were used to determine minimal critical domains most likely to contain genes or regulatory elements involved in heart development. This approach was used to refine the critical regions responsible for cardiac defects associated with chromosomal imbalances involving 1q24.2, 2q31.1, 15q26.3, and 22q11.2. CONCLUSIONS: The pattern of chromosomal imbalances in patients with congenital cardiac defects suggests that many loci may be involved in normal heart development, some with very strong and direct effects and others with less direct effects. Chromosomal duplication/deletion mapping will provide an important roadmap for genome-wide sequencing and genetic mapping strategies to identify novel genes critical for heart development.


Subject(s)
Chromosome Aberrations , Heart Defects, Congenital/genetics , Heart/embryology , Humans , Organogenesis/genetics
12.
Cell Rep ; 7(6): 1858-66, 2014 Jun 26.
Article in English | MEDLINE | ID: mdl-24931603

ABSTRACT

High-throughput gene expression analysis has revealed a plethora of previously undetected transcripts in eukaryotic cells. In this study, we investigate >1,100 unannotated transcripts in yeast predicted to lack protein-coding capacity. We show that a majority of these RNAs are enriched on polyribosomes akin to mRNAs. Ribosome profiling demonstrates that many bind translocating ribosomes within predicted open reading frames 10-96 codons in size. We validate expression of peptides encoded within a subset of these RNAs and provide evidence for conservation among yeast species. Consistent with their translation, many of these transcripts are targeted for degradation by the translation-dependent nonsense-mediated RNA decay (NMD) pathway. We identify lncRNAs that are also sensitive to NMD, indicating that translation of noncoding transcripts also occurs in mammals. These data demonstrate transcripts considered to lack coding potential are bona fide protein coding and expand the proteome of yeast and possibly other eukaryotes.


Subject(s)
Open Reading Frames , Polyribosomes/metabolism , RNA, Fungal/genetics , RNA, Messenger/genetics , Saccharomyces cerevisiae/genetics , Animals , Gene Expression Regulation, Fungal , Protein Biosynthesis , RNA Stability , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
13.
Hum Mol Genet ; 23(16): 4272-84, 2014 Aug 15.
Article in English | MEDLINE | ID: mdl-24694933

ABSTRACT

Recurrent 2q13 deletion syndrome is associated with incompletely penetrant severe cardiac defects and craniofacial anomalies. We used an atypical, overlapping 1.34 Mb 2q13 deletion in a patient with pathogenically similar congenital heart defects (CHD) to narrow the putative critical region for CHD to 474 kb containing six genes. To determine which of these genes is responsible for severe cardiac and craniofacial defects noted in the patients with the deletions, we used zebrafish morpholino knockdown to test the function of each orthologue during zebrafish development. Morpholino-antisense-mediated depletion of fibulin-7B, a zebrafish orthologue of fibulin-7 (FBLN7), resulted in cardiac hypoplasia, deficient craniofacial cartilage deposition and impaired branchial arch development. TMEM87B depletion likewise resulted in cardiac hypoplasia but with preserved branchial arch development. Depletion of both fibulin-7B and TMEM87B resulted in more severe defects of cardiac development, suggesting that their concurrent loss may enhance the risk of a severe cardiac defect. We postulate that heterozygous loss of FBLN7 and TMEM87B account for some of the clinical features, including cardiac defects and craniofacial abnormalities associated with 2q13 deletion syndrome.


Subject(s)
Calcium-Binding Proteins/deficiency , Chromosome Deletion , Chromosomes, Human, Pair 2 , Craniofacial Abnormalities/genetics , Heart Defects, Congenital/genetics , Membrane Proteins/deficiency , Zebrafish Proteins/genetics , Animals , Calcium-Binding Proteins/genetics , Female , Humans , Infant, Newborn , Male , Membrane Proteins/genetics , Morpholinos , Oligonucleotides, Antisense , Syndrome , Zebrafish/embryology , Zebrafish/genetics
14.
Nat Rev Mol Cell Biol ; 14(11): 699-712, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24105322

ABSTRACT

The increased application of transcriptome-wide profiling approaches has led to an explosion in the number of documented long non-coding RNAs (lncRNAs). While these new and enigmatic players in the complex transcriptional milieu are encoded by a significant proportion of the genome, their functions are mostly unknown. Early discoveries support a paradigm in which lncRNAs regulate transcription via chromatin modulation, but new functions are steadily emerging. Given the biochemical versatility of RNA, lncRNAs may be used for various tasks, including post-transcriptional regulation, organization of protein complexes, cell-cell signalling and allosteric regulation of proteins.


Subject(s)
RNA, Long Noncoding/metabolism , Animals , Genetic Variation/genetics , Genetic Variation/physiology , Humans , Models, Biological , RNA, Long Noncoding/genetics
15.
Mol Cell ; 45(3): 279-91, 2012 Feb 10.
Article in English | MEDLINE | ID: mdl-22226051

ABSTRACT

Decapping represents a critical control point in regulating expression of protein coding genes. Here, we demonstrate that decapping also modulates expression of long noncoding RNAs (lncRNAs). Specifically, levels of >100 lncRNAs in yeast are controlled by decapping and are degraded by a pathway that occurs independent of decapping regulators. We find many lncRNAs degraded by DCP2 are expressed proximal to inducible genes. Of these, we show several genes required for galactose utilization are associated with lncRNAs that have expression patterns inversely correlated with their mRNA counterpart. Moreover, decapping of these lncRNAs is critical for rapid and robust induction of GAL gene expression. Failure to destabilize a lncRNA known to exert repressive histone modifications results in perpetuation of a repressive chromatin state that contributes to reduced plasticity of gene activation. We propose that decapping and lncRNA degradation serve a vital role in transcriptional regulation specifically at inducible genes.


Subject(s)
Exoribonucleases/genetics , Gene Expression Regulation, Fungal , RNA Caps/metabolism , RNA Processing, Post-Transcriptional , RNA, Untranslated/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae/genetics , Endoribonucleases/genetics , Endoribonucleases/metabolism , Exoribonucleases/metabolism , Gene Knockout Techniques , Promoter Regions, Genetic , RNA Caps/genetics , RNA Stability , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Untranslated/genetics , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae Proteins/metabolism , Sequence Analysis, RNA
16.
Enzymes ; 31: 97-114, 2012.
Article in English | MEDLINE | ID: mdl-27166442

ABSTRACT

The degradation of RNA is a critical aspect of gene regulation. Correspondingly, ribonucleases exist within the cell to degrade RNA in specific cellular contexts. An important and conserved ribonuclease is called XRN1. This enzyme, an exoribonuclease, degrades RNA in a processive 5' to 3' direction. Substrates for XRN1 include decapped mRNA, endonucleolytically cleaved mRNA, lncRNA, and some aberrant tRNAs. In addition, XRN1 serves a vital role in the processing and maturation of the 5' ends of rRNA and snoRNAs. In this review, we discuss some of the important roles of XRN1 in the cell.

17.
Mol Cell ; 40(3): 349-50, 2010 Nov 12.
Article in English | MEDLINE | ID: mdl-21070961

ABSTRACT

With most of the important players identified, the process of decapping is thought, for the most part, to be well understood. In this issue of Molecular Cell, Song et al. (2010) challenge this notion with the identification of a previously uncharacterized mRNA decapping enzyme.

18.
RNA ; 16(11): 2226-38, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20826700

ABSTRACT

We have previously shown that a base-paired complex formed by two of the spliceosomal RNA components, U6 and U2 small nuclear RNAs (snRNAs), can catalyze a two-step splicing reaction that depended on an evolutionarily invariant region in U6, the ACAGAGA box. Here we further analyze this RNA-catalyzed reaction and show that while the 5' and 3' splice site substrates are juxtaposed and positioned near the ACAGAGA sequence in U6, the role of the snRNAs in the reaction is beyond mere juxtaposition of the substrates and likely involves the formation of a sophisticated active site. Interestingly, the snRNA-catalyzed reaction is metal dependent, as is the case with other known splicing RNA enzymes, and terbium(III) cleavage reactions indicate metal binding by the U6/U2 complex within the evolutionarily conserved regions of U6. The above results, combined with the structural similarities between U6 and catalytically critical domains in group II self-splicing introns, suggest that the base-paired complex of U6 and U2 snRNAs is a vestigial ribozyme and a likely descendant of a group II-like self-splicing intron.


Subject(s)
Magnesium/metabolism , RNA, Catalytic/metabolism , RNA, Small Nuclear/metabolism , Base Sequence , Exons , Humans , Molecular Sequence Data , RNA Splicing , Substrate Specificity
19.
J Biomed Biotechnol ; 2010: 624719, 2010.
Article in English | MEDLINE | ID: mdl-20467587

ABSTRACT

The intercalated disk (ID) is a complex structure that electromechanically couples adjoining cardiac myocytes into a functional syncitium. The integrity of the disk is essential for normal cardiac function, but how the diverse elements are assembled into a fully integrated structure is not well understood. In this study, we examined the assembly of new IDs in primary cultures of adult rat cardiac myocytes. From 2 to 5 days after dissociation, the cells flatten and spread, establishing new cell-cell contacts in a manner that recapitulates the in vivo processes that occur during heart development and myocardial remodeling. As cells make contact with their neighbors, transmembrane adhesion proteins localize along the line of apposition, concentrating at the sites of membrane attachment of the terminal sarcomeres. Cx43 gap junctions and ankyrin-G, an essential cytoskeletal component of voltage gated sodium channel complexes, were secondarily recruited to membrane domains involved in cell-cell contacts. The consistent order of the assembly process suggests that there are specific scaffolding requirements for integration of the mechanical and electrochemical elements of the disk. Defining the relationships that are the foundation of disk assembly has important implications for understanding the mechanical dysfunction and cardiac arrhythmias that accompany alterations of ID architecture.


Subject(s)
Armadillo Domain Proteins/metabolism , Intercellular Junctions/metabolism , Myocytes, Cardiac/metabolism , Animals , Cadherins/metabolism , Cells, Cultured , Connexin 43/metabolism , Cytoskeleton/metabolism , Female , Histocytochemistry , Intercellular Junctions/ultrastructure , Microscopy, Fluorescence , Myocytes, Cardiac/cytology , Rats , Sodium Channels/metabolism
20.
Proc Natl Acad Sci U S A ; 106(29): 11901-6, 2009 Jul 21.
Article in English | MEDLINE | ID: mdl-19549866

ABSTRACT

Pre-mRNA splicing is a crucial step in eukaryotic gene expression and is carried out by a highly complex ribonucleoprotein assembly, the spliceosome. Many fundamental aspects of spliceosomal function, including the identity of catalytic domains, remain unknown. We show that a base-paired complex of U6 and U2 small nuclear RNAs, in the absence of the approximately 200 other spliceosomal components, performs a two-step reaction with two short RNA oligonucleotides as substrates that results in the formation of a linear RNA product containing portions of both oligonucleotides. This reaction, which is chemically identical to splicing, is dependent on and occurs in proximity of sequences known to be critical for splicing in vivo. These results prove that the complex formed by U6 and U2 RNAs is a ribozyme and can potentially carry out RNA-based catalysis in the spliceosome.


Subject(s)
Biocatalysis , Proteins/metabolism , RNA Splicing/genetics , RNA, Small Nuclear/metabolism , Base Sequence , Esterification , Humans , Molecular Sequence Data , Nucleic Acid Conformation , Oligonucleotides/genetics , RNA, Small Nuclear/chemistry , RNA, Small Nuclear/genetics , Spliceosomes/metabolism , Substrate Specificity
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