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1.
Vet Pathol ; 52(3): 580-95, 2015 May.
Article in English | MEDLINE | ID: mdl-25161209

ABSTRACT

Mice deficient in TMEM218 (Tmem218(-/-) ) were generated as part of an effort to identify and validate pharmaceutically tractable targets for drug development through large-scale phenotypic screening of knockout mice. Routine diagnostics, expression analysis, histopathology, and electroretinogram analyses completed on Tmem218(-/-) mice identified a previously unknown role for TMEM218 in the development and function of the kidney and eye. The major observed phenotypes in Tmem218(-/-) mice were progressive cystic kidney disease and retinal degeneration. The renal lesions were characterized by diffuse renal cyst development with tubulointerstitial nephropathy and disruption of tubular basement membranes in essentially normal-sized kidneys. The retinal lesions were characterized by slow-onset loss of photoreceptors, which resulted in reduced electroretinogram responses. These renal and retinal lesions are most similar to those associated with nephronophthisis (NPHP) and retinitis pigmentosa in humans. At least 10% of NPHP cases present with extrarenal conditions, which most often include retinal degeneration. Senior-Løken syndrome is characterized by the concurrent development of autosomal recessive NPHP and retinitis pigmentosa. Since mutations in the known NPHP genes collectively account for only about 30% of NPHP cases, it is possible that TMEM218 could be involved in the development of similar ciliopathies in humans. In reviewing all other reported mouse models of NPHP, we suggest that Tmem218(-/-) mice could provide a useful model for elucidating the pathogenesis of cilia-associated disease in both the kidney and the retina, as well as in developing and testing novel therapeutic strategies for Senior-Løken syndrome.


Subject(s)
Disease Models, Animal , Kidney Diseases, Cystic/veterinary , Leber Congenital Amaurosis/veterinary , Membrane Proteins/genetics , Mice, Knockout/genetics , Optic Atrophies, Hereditary/veterinary , Retinal Degeneration/veterinary , Animals , Ciliopathies , Electroretinography/veterinary , Eye/pathology , Female , Kidney/pathology , Kidney Diseases, Cystic/genetics , Kidney Diseases, Cystic/pathology , Leber Congenital Amaurosis/pathology , Male , Membrane Proteins/physiology , Mice , Optic Atrophies, Hereditary/pathology , Retina/pathology , Retinal Degeneration/genetics
2.
Vet Pathol ; 46(2): 313-24, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19261645

ABSTRACT

UDP-GlcNAc: lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase) is an alpha(2)beta(2)gamma(2) hexameric enzyme that catalyzes the first step in the synthesis of the mannose 6-phosphate targeting signal on lysosomal hydrolases. In humans, mutations in the gene encoding the alpha/beta subunit precursor give rise to mucolipidosis II (MLII), whereas mutations in the gene encoding the gamma subunit cause the less severe mucolipidosis IIIC (MLIIIC). In this study we describe the phenotypic, histologic, and serum lysosomal enzyme abnormalities in knockout mice lacking the gamma subunit and compare these findings to those of mice lacking the alpha/beta subunits and humans with MLII and MLIIIC. We found that both lines of mutant mice had elevated levels of serum lysosomal enzymes and cytoplasmic alterations in secretory cells of several exocrine glands; however, lesions in gamma-subunit deficient (Gnptg(-/-)) mice were milder and more restricted in distribution than in alpha/beta-subunit deficient (Gnptab(-/-)) mice. We found that onset, extent, and severity of lesions that developed in these two different knockouts correlated with measured lysosomal enzyme activity; with a more rapid, widespread, and severe storage disease phenotype developing in Gnptab(-/-) mice. In contrast to mice deficient in the alpha/beta subunits, the mice lacking the gamma subunits were of normal size, lacked cartilage defects, and did not develop retinal degeneration. The milder disease in the gamma-subunit deficient mice correlated with residual synthesis of the mannose 6-phosphate recognition marker. Of significance, neither strain of mutant mice developed cytoplasmic vacuolar inclusions in fibrocytes or mesenchymal cells (I-cells), the characteristic lesion associated with the prominent skeletal and connective tissue abnormalities in humans with MLII and MLIII. Instead, the predominant lesions in both lines of mice were found in the secretory epithelial cells of several exocrine glands, including the pancreas, and the parotid, submandibular salivary, nasal, lacrimal, bulbourethral, and gastric glands. The absence of retinal and chondrocyte lesions in Gnptg(-/-) mice might be attributed to residual beta-glucuronidase activity. We conclude that mice lacking either alpha/beta or gamma subunits displayed clinical and pathologic features that differed substantially from those reported in humans having mutations in orthologous genes.


Subject(s)
Mucolipidoses/classification , Mucolipidoses/pathology , Animals , Female , Hydrolases/metabolism , Lysosomes/enzymology , Male , Mice , Mice, Knockout , Mucolipidoses/genetics , Mutation , Phenotype , Protein Binding , Transferases (Other Substituted Phosphate Groups)/genetics , Transferases (Other Substituted Phosphate Groups)/metabolism
3.
Invest Ophthalmol Vis Sci ; 42(11): 2706-13, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11581219

ABSTRACT

PURPOSE: To investigate how the differentiation of ARPE-19 cells affects the relative expression of the FGFR genes in response to oxidative stress. METHODS: After differentiation in vitro, APRE-19 cells were treated with t-butyl hydroperoxide (tBH) or hydrogen peroxide (H2O2) to induce oxidative stress. Viability and reactive oxygen intermediate (ROI) production were measured using standard assays. The mRNA expression of FGFR1, FGFR2, cellular retinaldehyde-binding protein (CRALBP), RPE65, and heme oxygenase-1 (HO-1) were measured by Northern blot analysis as a function of treatment with tBH and H2O2. RESULTS: ARPE-19 cells were viable at all tBH concentrations tested but showed progressive loss of viability at concentrations greater than 300 microM H2O2. Differentiated ARPE-19 cells treated with tBH or H2O2 resulted in upregulation of the HO-1 and FGFR1 transcripts. The expression of RPE-differentiated specific genes, including FGFR2, CRALBP, and RPE65 mRNAs, was downregulated with tBH or H2O2 treatment. CONCLUSIONS: Oxidative stress in differentiated ARPE-19 cells alters the expression of FGFR1, FGFR2, CRALBP, and RPE65 toward levels characteristic of the undifferentiated state. If similar changes take place in vivo, these events could alter the proliferative potential, viability, and even the function of the RPE.


Subject(s)
Eye Proteins/genetics , Gene Expression , Oxidative Stress , Retinal Ganglion Cells/metabolism , Blotting, Northern , Carrier Proteins/genetics , Carrier Proteins/metabolism , Cell Differentiation , Cell Line , Down-Regulation , Eye Proteins/metabolism , Heme Oxygenase (Decyclizing)/genetics , Heme Oxygenase (Decyclizing)/metabolism , Heme Oxygenase-1 , Humans , Hydrogen Peroxide/pharmacology , Membrane Proteins , Proteins/genetics , Proteins/metabolism , RNA, Messenger/metabolism , Receptor Protein-Tyrosine Kinases/genetics , Receptor Protein-Tyrosine Kinases/metabolism , Receptor, Fibroblast Growth Factor, Type 1 , Receptor, Fibroblast Growth Factor, Type 2 , Receptors, Fibroblast Growth Factor/genetics , Receptors, Fibroblast Growth Factor/metabolism , Retinal Ganglion Cells/cytology , Retinal Ganglion Cells/drug effects , Time Factors , cis-trans-Isomerases , tert-Butylhydroperoxide/pharmacology
4.
Curr Eye Res ; 23(3): 226-31, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11803485

ABSTRACT

PURPOSE: The purpose of this study is to demonstrate the effect of culture density on the steady state mRNA levels of fibroblast growth factor-2 (FGF-2) when retinal pigment epithelial (RPE) cells are subjected to oxidative stress in vitro. METHODS: Subconfluent and confluent cultures of the established RPE cell line ARPE-19, were treated with increasing concentrations of tert-butyl hydroperoxide (tBH) or hydrogen peroxide (H(2)O(2)). Cell viability was measured using the WST-1 assay, and intracellular reactive oxygen intermediate (ROI) production was quantified by dichlorofluoroscein (DCF) fluorescence. Steady state changes in heme oxygenase-1 (HO-1) and FGF-2 mRNAs were measured by Northern blot analysis. RESULTS: Confluent cultures of ARPE-19 cells were less susceptible than subconfluent cultures to the toxic effects of the chemical oxidants. The intracellular reactive oxygen intermediate production was higher in subconfluent than confluent cultures with increasing tBH concentration. At nontoxic concentrations of tBH and H(2)O(2), a dose dependent increase in FGF-2 expression was seen as a function of culture density. FGF-2 mRNA expression was induced after tBH treatment in subconfluent, but not confluent cells. On the other hand, FGF-2 mRNA induction was observed after H(2)O( 2) treatment in confluent, but not subconfluent cultures. In contrast, no density dependent induction of HO-1 mRNA was seen after treatment with either tBH or H(2)O(2). CONCLUSIONS: These results suggest that care should be taken to control for cell density in similar types of in vitro experiments.


Subject(s)
Cell Count , Fibroblast Growth Factor 2/biosynthesis , Oxidative Stress/physiology , Pigment Epithelium of Eye/cytology , Blotting, Northern , Cell Survival , Cells, Cultured , Dose-Response Relationship, Drug , Fibroblast Growth Factor 2/genetics , Heme Oxygenase (Decyclizing)/genetics , Heme Oxygenase (Decyclizing)/metabolism , Heme Oxygenase-1 , Humans , Hydrogen Peroxide/pharmacology , Membrane Proteins , Pigment Epithelium of Eye/drug effects , Pigment Epithelium of Eye/metabolism , RNA, Messenger/metabolism , Reactive Oxygen Species , tert-Butylhydroperoxide/pharmacology
5.
Invest Ophthalmol Vis Sci ; 41(8): 2357-62, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10892884

ABSTRACT

PURPOSE: The expression and alternative splicing of the four FGF receptor (FGFR) mRNAs are regulated in a developmental- and tissue-specific fashion. Capability of differentiation in vitro of the retinal pigment epithelial cell line ARPE-19 has been previously demonstrated. In this study, the hypothesis that FGF receptor gene expression and the alternative splicing of the FGFR1 mRNA is regulated as a function of ARPE-19 differentiation in vitro was tested. METHODS: ARPE-19 cells were plated at sparse or confluent densities and maintained in culture up to 14 months. The expression of FGF receptors and the ratio of the FGFR1beta to FGFR1alpha splice variants of the FGFR1 transcript were quantified by a published PCR technique. Two in vivo samples of human RPE served as controls. RESULTS: Sparse cultures of ARPE-19 cells predominantly express FGFR1. When these cultures are allowed to differentiate, FGFR2 is also expressed. Samples of mRNA from RPE cells in vivo exhibit FGFR1 and FGFR2 expression as well as FGFR3 expression, a form that is minimally apparent in vitro. The ratio of the FGFR1beta to FGFR1alpha splice variant decreases as a function of cell differentiation in vitro and approaches the ratio observed in human RPE cells in vivo. Stimulation of cultures in vitro with FGF2 as a prototypical differentiation agent does not regulate the ratio of the FGFR1beta to FGFR1alpha splice variant. CONCLUSIONS: Differentiation of the ARPE-19 cell line in vitro recapitulates many but not all the in vivo patterns of FGFR expression and splicing. This in vitro system may be useful for selected studies on how cellular differentiation regulates FGF receptor gene expression and splicing.


Subject(s)
Alternative Splicing/genetics , Cell Differentiation/physiology , Pigment Epithelium of Eye/cytology , RNA, Messenger/biosynthesis , Receptors, Fibroblast Growth Factor/genetics , Blotting, Northern , Cell Line , Gene Expression , Growth Substances/pharmacology , Half-Life , Humans , Pigment Epithelium of Eye/drug effects , Pigment Epithelium of Eye/metabolism , Receptors, Fibroblast Growth Factor/biosynthesis , Recombinant Proteins/pharmacology , Reverse Transcriptase Polymerase Chain Reaction
6.
Mol Vis ; 5: 39, 1999 Dec 29.
Article in English | MEDLINE | ID: mdl-10617776

ABSTRACT

PURPOSE: To explore the changes in expression of a set of genes in a single retinal pigment epithelial (RPE) cell line and two fibroblast cell lines as controls under culture conditions previously used for the analysis of senescent gene expression. METHODS: A single human RPE cell line, which had previously been characterized using known markers of senescence, and two fibroblast cell lines were grown to replicative exhaustion. The mRNA phenotype of genes known to be altered by senescence were studied by quantitative Northern analysis. RESULTS: The mRNA phenotype of cells changes at replicative senescence yielding a synthetic phenotype which is similar to cells found in repairing wounds. Of the genes studied, urokinase-type plasminogen activator and plasminogen activator inhibitor-1 were regulated in RPE cells similar to fibroblasts at senescence. The largest changes noted for any single gene were the upregulation of insulin growth factor binding protein 2, and the downregulation of collagen I alpha 2, basic fibroblast growth factor, and fibroblast growth factor-5. CONCLUSIONS: This study demonstrates an altered mRNA phenotype of a human RPE cell line grown to replicative exhaustion. This analysis of a single cell line emphasizes the variability of results based on a single cell line or tissue specimen and indicates the need for additional study.


Subject(s)
Eye Proteins , Heparan Sulfate Proteoglycans , Nerve Growth Factors , Pigment Epithelium of Eye/cytology , Pigment Epithelium of Eye/metabolism , Blotting, Northern , Cell Division , Cell Line , Cellular Senescence , Collagen/metabolism , Collagen Type I , Fibroblast Growth Factor 2/metabolism , Fibroblast Growth Factor 5 , Fibroblast Growth Factors/metabolism , Fibroblasts/cytology , Fibroblasts/metabolism , Gene Expression , Heparitin Sulfate/metabolism , Humans , Insulin-Like Growth Factor Binding Protein 2/metabolism , Plasminogen Activator Inhibitor 1/metabolism , Proteins/metabolism , Proteoglycans/metabolism , RNA, Messenger/metabolism , Serpins/metabolism , Tissue Inhibitor of Metalloproteinase-3/metabolism , Urokinase-Type Plasminogen Activator/metabolism
7.
Exp Eye Res ; 67(2): 151-62, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9733582

ABSTRACT

Differentiated retinal pigment epithelial (RPE) cells in vivo express basal levels of FGF-5, a secreted member of the FGF gene family. RPE cells proliferate in response to pathological events, resulting in a transient increase in FGF-5 gene expression. The goal of this study is to identify cis-acting sequences in the FGF-5 gene promoter which upregulate FGF-5 gene expression when differentiated RPE cells enter the cell cycle and proliferate. In vitro cultures of RPE cells were transfected with various FGF-5 promoter/luciferase deletion constructs, using methods specifically optimized for proliferating and differentiated RPE cells. A proximal promoter/enhancer whose activity is not cell-context dependent was identified between FGF-5 sequences -314 and +48. In addition, a silencer element (-1256/-883) was identified in the distal region which is active only in differentiated RPE cells. When tested in a heterologous system, the same element had silencer activity in differentiated cells. Two small regions in the distal FGF-5 gene promoter, -1195/-1173 and -984/-967 were able to specifically bind to nuclear proteins from differentiated RPE cells but not from proliferating RPE cells as evidenced by gel mobility shift assays. Therefore, FGF-5 gene expression in the RPE may be regulated by the formation of differentiation-specific complexes.


Subject(s)
Fibroblast Growth Factors/genetics , Pigment Epithelium of Eye/metabolism , Promoter Regions, Genetic , Base Sequence , Cell Culture Techniques , Cell Differentiation/genetics , Cell Division/genetics , Cell Line , DNA Fragmentation , Fibroblast Growth Factor 5 , Fibroblast Growth Factors/metabolism , Gene Expression Regulation , Humans , Luciferases/genetics , Luciferases/metabolism , Molecular Sequence Data , Nuclear Proteins/metabolism , Pigment Epithelium of Eye/cytology , Restriction Mapping , Transfection
8.
Mol Cell Biol ; 13(4): 2623-33, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8455635

ABSTRACT

The GCR1 gene product is required for maximal transcription of many yeast genes including genes encoding glycolytic enzymes. Transcription of the yeast enolase gene ENO2 is reduced 50-fold in strains carrying a gcr1 null mutation. cis-acting sequences that are sufficient for GCR1-dependent regulation of ENO2 expression were identified by using an enhancerless CYC1 promoter which is not normally dependent on GCR1 for expression. A 60-bp ENO2 sequence that was sufficient to provide high-level, GCR1-dependent transcriptional activation of the CYC1 promoter was identified. This 60-bp element could be subdivided into a 30-bp sequence containing a novel RAP1-binding site and a GCR1-binding site which did not activate CYC1 transcription and a 30-bp sequence containing a novel enhancer element that conferred moderate levels of GCR1-independent transcriptional activation. The 60-bp CGCR1-dependent upstream activator sequence is located immediately downstream from previously mapped overlapping binding sites for the regulatory proteins ABFI and RAP1. Evidence is presented that the overlapping ABFI- and RAP1-binding sites function together with sequences that bind GCR1 and RAP1 to stage transcriptional activation of ENO2 expression.


Subject(s)
DNA-Binding Proteins/genetics , Fungal Proteins/genetics , Gene Expression Regulation, Fungal , Genes, Fungal , Genes, Regulator , Phosphopyruvate Hydratase/genetics , Regulatory Sequences, Nucleic Acid , Saccharomyces cerevisiae/genetics , Base Sequence , Binding Sites , DNA Mutational Analysis , Glycolysis , Molecular Sequence Data , Oligodeoxyribonucleotides/chemistry , Promoter Regions, Genetic , RNA, Messenger/genetics , Saccharomyces cerevisiae Proteins , Transcription Factors , Transcription, Genetic
9.
Biochemistry ; 30(4): 1057-62, 1991 Jan 29.
Article in English | MEDLINE | ID: mdl-1989676

ABSTRACT

Site-directed mutagenesis has been used to examine the chemical roles of four active-site residues in histidine decarboxylase (HDC) from Lactobacillus 30a. This protein is known to undergo an autoactivation in which chain cleavage between serines-81 and -82 leads to cofactor (pyruvoyl) formation at position 82. Conversion of Ser-81 to Ala virtually eliminates productive cleavage. It is proposed that the residue plays a key role in stabilizing the transition state of the chain cleavage reaction. Conversion of Phe-83 to Met renders the proenzyme thermally less stable than wild type and appears to slightly increase the rate of autoactivation. The Km value for histidine is increased about 8-fold, confirming crystallographic evidence that Phe-83 is involved in substrate binding. Both wild-type and F83M enzymes show constant Km and steadily increasing kcat values as a function of temperature. Lys-155 and Tyr-262, by virtue of their positions in the active site of HDC, have been proposed to possibly play specific roles in either autoactivation or catalysis by active HDC. Conversion to Gln and Phe respectively suggests that these residues have real but minor roles in those processes.


Subject(s)
Histidine Decarboxylase/chemistry , Binding Sites , Catalysis , Coenzymes/metabolism , Enzyme Activation , Escherichia coli/genetics , Histidine Decarboxylase/genetics , Kinetics , Lactobacillus/enzymology , Mutagenesis, Site-Directed , Pyruvates/metabolism , Substrate Specificity
10.
Biochemistry ; 29(6): 1648-54, 1990 Feb 13.
Article in English | MEDLINE | ID: mdl-2334723

ABSTRACT

Potential probes of protein cholesterol and fatty acid binding sites, namely, 12-[(5-iodo-4-azido-2-hydroxybenzoyl)amino]dodecanoate (IFA) and its coenzyme A (IFA:CoA) and cholesteryl (IFA:CEA) esters, were synthesized. These radioactive, photoreactive lipid analogues were recognized as substrates and inhibitors of acyl-CoA:cholesterol O-acyltransferase (ACAT) and cholesterol esterase, neutral lipid binding enzymes which are key elements in the regulation of cellular cholesterol metabolism. In the dark, IFA reversibly inhibited cholesteryl [14C]oleate hydrolysis by purified bovine pancreatic cholesterol esterase with an apparent Ki of 150 microM. Cholesterol esterase inhibition by IFA became irreversible after photolysis with UV light and oleic acid (1 mM) provided 50% protection against inactivation. Incubation of homogeneous bovine pancreatic cholesterol esterase with IFA:CEA resulted in its hydrolysis to IFA and cholesterol, indicating recognition of IFA:CEA as a substrate by cholesterol esterase. The coenzyme A ester, IFA:CoA, was a reversible inhibitor of microsomal ACAT activity under dark conditions (apparent Ki = 20 microM), and photolysis resulted in irreversible inhibition of enzyme activity with 87% efficiency. IFA:CoA was also recognized as a substrate by both liver and aortic microsomal ACATs, with resultant synthesis of 125IFA:CEA. IFA and its derivatives, IFA:CEA and IFA:CoA, are thus inhibitors and substrates for cholesterol esterase and ACAT. Biological recognition of these photoaffinity lipid analogues will facilitate the identification and structural analysis of hitherto uncharacterized protein lipid binding sites.


Subject(s)
Acyl Coenzyme A/metabolism , Azides/metabolism , Carboxylic Ester Hydrolases/metabolism , Cholesterol Esters/metabolism , Laurates/metabolism , Lauric Acids/metabolism , Pancreas/enzymology , Sterol Esterase/metabolism , Sterol O-Acyltransferase/metabolism , Aorta/enzymology , Sterol O-Acyltransferase/antagonists & inhibitors
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