[Activity of glutamate dehydrogenase, gamma-glutamyltranspeptidase and creatine kinase in saliva in gingivitis]. / Aktivnost' glutamatdegidrogenazy, gamma-glutamiltranspeptidazy i kreatinkinazy v sliune pri vospalenii desny.

In the human saliva (oral fluid), the activity of glutamate dehydrogenase tends to decrease in gingivitis and diminishes in periodontitis. That of gamma-glutamyl transpeptidase and creatine kinase increases in gingivitis. In periodontitis, their activity is higher than that in gingivitis. The above changes in enzymatic activities reflect metabolic changes in the gingiva and periodontium in inflammation. The saliva from the salivary ducts shows no activity of the above enzymes. They enter the mixed saliva (oral fluid) together with epithelial and other oral tissue cells, with leukocytes and microbes which manifold in gingivitis.

[Iron in oral cavity fluid in gingival inflammation]. / Zhelezo zhidkostei polosti rta pri vospalenii desny.

In parodontosis, iron levels are higher in the human gingival fluid and mixed saliva. The free iron-binding capacity of saliva is also increased. In gingivitis, higher levels of iron and free binding capacity are less evident. The increased levels of iron activate free radical oxidation in gingival tissue and improve microbial metabolism. This all contributes to the deterioration of a pathological process in the gingiva.

[Adenylate cyclase and guanylate cyclase in the saliva of healthy persons and in periodontitis]. / Adenilattsiklaza i guanilattsiklaza v sliune zdovorykh liudei i pri parodontite.

Studies of mixed salivary pools of normal subjects aged 22-35 and of patients with periodontitis of slight and medium degree, aged 25-55, have shown a 1.5 times lower activity of salivary adenylate cyclase in the patients with slight periodontitis as against normal subjects and an almost two times lower activity of this enzyme in medium-severity periodontitis patients as against those with the slight condition. The directions of changes in guanylate cyclase activities are contrary; this enzyme activity is elevated in the patients with the slight-degree condition vs. the norm, and is still more elevated in those with the medium-severity disease. Some sex-associated differences do not influence the total regularity of adenylate cyclase activity reduction and guanylate cyclase activity elevation, augmenting with the severity of the pathologic process in the periodontium. The mechanism of action of these two cyclases in periodontitis is discussed, as is the necessity of paying due attention to changes in their activities when analyzing the pathogenesis of periodontitis.

[Effects of saccharose diet and drinking water with different mineral composition on calcium-phosphorus metabolism in the teeth and bones of rats]. / Vliianie sakharoznoi diety i pit'evoi vody razlichnogo mineral'nogo sostava na fosforno-kal'tsievyi obmen v zubakh i kostiakh krys.

Metabolism of 32P and 45Ca in the teeth and bones was studied in 140 Wistar rats that received a high-saccharose diet and varying amounts of Ca, Mg and F in drinking water. The water hardness and F content influence 45Ca and 32P metabolism in the teeth and bones and caries development. 45Ca and 32P inclusion into mineralized tissues was decreased in rats fed with the high-saccharose diet as compared to the control animals which received a usual ration. Excessive consumption of Ca and Mg with drinking water was conductive to mineralization. The level of 45Ca and 32P inclusion in these groups was closer to the control. Fluorination of drinking water of varying hardness decreased caries development and increased 32P and 45Ca inclusion into mineralized tissues.

[The effect of magnesium sulfate electrophoresis and galvanization on the mineralization of teeth and bones]. / Vliianie élektroforeza magniia sulfata i gal'vanizatsii na mineralizatsiiu zubov i kostei.

Effects of Mg2+ electrophoresis and galvanization on tooth and bone mineralization was experimentally studied with the use of radioactive Ca and P isotopes. Mg2+ electrophoresis and, to a lesser degree, galvanization enhanced 32P incorporation in incisors and maxillary bones. Mg2+ significantly increased 45Ca incorporation in teeth and maxillary bones. Experimental data permit clinical trials of Mg2+ efficacy in patients with disordered mineralization and remineralization.

[The effect of local exposure to Mg and heat on the biosynthesis of sulfur-containing compounds in the oral tissues]. / Vliianie lokal'nogo vozdeistviia Mg i tepla na biosintez serosoderzhashchikh soedinenii tkanei polosti rta.

Local Mg electrophoresis was shown to considerably increase the radiolabeled sulphate contents in an application area: gums, mandibular alveolar processes, lower incisors and molars roots. In areas far beyond the phoresis sites (femoral epiphyses and diaphyses) label incorporation was not affected. Mg-free galvanization had no effect on sulphate incorporation into the mineralized tissues. Heating up to 50 C substantially increased the S radioactivity in alveolar processes, molar and incisor roots. Simultaneous heating with Mg electrophoresis produced an additive effect.

[Iododeoxyuridine metabolism in the oral tissues and its alteration in infection by the herpes simplex virus]. / Obmen ioddezoksiuridina v tkaniakh polosti rta i ego izmenenie pri infitsirovanii virusom prostogo gerpesa.

In healthy white rats [125I]-desoxyuridine, [3H]-thymidine, 2-[14C]-uracyl, 8-[14C]-adenine and [3H)-uridine were used to demonstrate that the [125I]-desoxyuridine metabolism in oral mucosa is analogous to that of thymidine and different from metabolic pathways of another nucleoside uridine and nitrogen purine and pyrimidine bases of adenine and uracyl. In rats infected with herpes simplex virus, the relative tissue/blood 123I radioactivity increased after a [125I]-desoxyuridine injection. The authors suggest that the mechanism of [125I]-desoxyuridine action relies on its involvement into the viral host cell DNA.

[Correction of mineral and protein metabolism by anabolic preparations and vitamin D2 in rats with dental caries]. / Korrektsiia mineral'nogo i belkovogo obmena pri kariece zubov u krys anabolicheskimi preparatiami i vitaminom D2.

The effect of nerabol, namacyte and vitamin D2 introduction (separately or in combination) on the animal mineral and protein metabolism has been studied in the experiment on 210 white Wistar rats with reproduced caries. Biochemical and radioisotopic investigations have permitted determining the quantitative content of phosphorus, calcium, 14C-glycine in the saliva, blood serum, teeth, jaw bones and femur. The data obtained evidence for the pronounced caries-static effect of the tested preparations connected with their active influence on the mineral and protein metabolism of experimental animals as well as for the expediency to use the above drugs for complex prophylaxis and treatment of dental caries normalizing processes.

[Age-related characteristics of the effect of deafferentation of the mandible on the metabolism of sulfate, citrate, phosphorus and calcium in the bones]. / Vozrastnye osobennosti vliianiia deafferentatsii nizhnecheliustnoi kosti na obmen v nei sul'fata, tsitrata, fosfora i kal'tsiia v kostiakh.

Mineral metabolism in the bones of 1- and 3-month rats reacts to a different extent to cutting the lower alveolar nerve. After deafferentation of the lower jaw, the decrease in the intensity of incorporation of the intraperitoneally injected [3-14C]-citrate and [35S]-sulphate in the bones is more significant in 1-month animals as compared to that in older ones. Deafferentation increased utilization of Na2H32PO4 and 45CaCl2 in the bones of younger rats, but decreased incorporation of 32P and remained unchanged incorporation of 45Ca in 3-month animals.

[Age-related changes in ATPase activity from rat salivary glands]. / Vozrastnye izmeneniia aktivnosti ATP-azy v sliunnykh zhelezakh krys.

The ATP hydrolysis by homogenates of oral cavity mucosa, parotid, mandibular and sublingual glands of rats was studied depending on the animal age (1,3 and 12 months). In all the studied tissues the souring of incubation medium due to ATP hydrolysis is followed by its subsequent alkalization up to the initial pH. The alkalization rate increases with the animal age and by 12 months reaches its constant value (within 22.5 +/- 4.1-25.7 +/- 7.5 ng-ion H3 per 1 mg of protein for 1 min). The observed effect evidences for an increase in the mitochondrial membrane permeability of the studied tissues with the animal age.

[Role of salivary ribonuclease in pathogenesis of periodontosis]. / Rol' ribonukleazy sliuny v patogeneze parodontoza

In patients with periodontal disease a distinct increase in secretion of salivary acidic and alkaline RNAases was found. The RNAases activity in mixed saliva correlated with the intensity of degradation of periodontal tissues during loss of alveolar layer in aged Wistar rats and the spontaneous periodontal disease in cats. In experiments with golden hamsters salivary RNAase was found to penetrate through mucosa in active form. The purified salivary RNAase inhibited the protein biosynthesis in rabbit gingiva. Intensive atrophic alterations were produced in periodontal tissues of Wistar rats by means of purified salivary RNAase.

[Role of salivary kallikrein in the pathogenesis of periodontosis]. / Rol' kallikreina sliuny v patogeneze parodontoza

In patients with parodontosis the distinct increase in salivary kallikrein secretion was found. In Wistar rats the intensity of age atrophy of alveoli dentalis correlated with BAEE-esterase activity of mixed saliva. Desalivation considerably decreased the rate of age atrophy of parodontosis in rats. Salivary kallikrein, penetrating in active form into parodontal tissues, caused the pronounced vasodilation in dogs and golden hamsters. By means of kallikrein preparations an intensive inflammatory-dystrophical process was reproduced in parodontal tissues of Wistar rats. The local treatment of patients with parodontosis by kallikrein inhibitors was found to be especially effective.

[Effect of prodigiozan on the course of candidiasis of the oral mucosa clinically and in an experiment]. / Vliianie prodigiozana na techenie kandidoza slizistoi obolochki polosti rta v klinike i éksperimente

The effect of various doses of prodigiozan on Candida infection was studied under experimental and clinical conditions. Hamsters and guinea pigs were used in the experiments. Prodigiozan was administered intramuscularly to the hamsters in doses of 100 gamma/kg of the body weight 1 hour before the infection and intracutaneously to the guinea pigs in doses of 50 gamma/kg 2 days before the infection. The results showed that the drug accelerated the recovery of the animals. In complex therapy of a group of 22 patients with candidosis of the mouth mucosa of various localization prodigiozan was administered thrice in doses of 50 gamma/kg. This provided faster elimination of the pathological process.