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1.
Microbiol Spectr ; 12(4): e0322023, 2024 Apr 02.
Article in English | MEDLINE | ID: mdl-38441979

ABSTRACT

Equid alphaherpesvirus 8 (EqHV-8) is one of the most economically important viruses that is known to cause severe respiratory disease, abortion, and neurological syndromes in equines. However, no effective vaccines or therapeutic agents are available to control EqHV-8 infection. Heme oxygenase-1 (HO-1) is an antioxidant defense enzyme that displays significant cytoprotective effects against different viral infections. However, the literature on the function of HO-1 during EqHV-8 infection is little. We explored the effects of HO-1 on EqHV-8 infection and revealed its potential mechanisms. Our results demonstrated that HO-1 induced by cobalt-protoporphyrin (CoPP) or HO-1 overexpression inhibited EqHV-8 replication in susceptible cells. In contrast, HO-1 inhibitor (zinc protoporphyria) or siRNA targeting HO-1 reversed the anti-EqHV-8 activity. Furthermore, biliverdin, a metabolic product of HO-1, mediated the anti-EqHV-8 effect of HO-1 via both the protein kinase C (PKC)ß/extracellular signal-regulated kinase (ERK)1/ERK2 and nitric oxide (NO)-dependent cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) signaling pathways. In addition, CoPP protected the mice by reducing the EqHV-8 infection in the lungs. Altogether, these results indicated that HO-1 can be developed as a promising therapeutic strategy to control EqHV-8 infection.IMPORTANCEEqHV-8 infections have threatened continuously donkey and horse industry worldwide, which induces huge economic losses every year. However, no effective vaccination strategies or drug against EqHV-8 infection until now. Our present study found that one host protien HO-1 restrict EqHV-8 replication in vitro and in vivo. Furthermore, we demonstrate that HO-1 and its metabolite biliverdin suppress EqHV-8 relication via the PKCß/ERK1/ERK2 and NO/cGMP/PKG pathways. Hence, we believe that HO-1 can be developed as a promising therapeutic strategy to control EqHV-8 infection.


Subject(s)
Cyclic GMP-Dependent Protein Kinases , Heme Oxygenase-1 , Horses , Animals , Mice , Heme Oxygenase-1/genetics , Heme Oxygenase-1/metabolism , Heme Oxygenase-1/pharmacology , Cyclic GMP-Dependent Protein Kinases/metabolism , Cyclic GMP-Dependent Protein Kinases/pharmacology , Biliverdine/pharmacology , Signal Transduction , Virus Replication
2.
Microb Pathog ; 186: 106506, 2024 Jan.
Article in English | MEDLINE | ID: mdl-38128702

ABSTRACT

Equid herpesvirus type 8 (EqHV-8) is known to cause abortion, respiratory signs, and viral encephalitis in equines. EqHV-8 has been reported to cause serious economic losses in large-scale donkey farms in China. However, little is known about the viral replication and immune reaction in the brains and lungs of EqHV-8-induced C57BL/6J mice. We determined the pathogenicity and immune status in a mice model. The C57BL/6J mice were infected with the EqHV-8 donkey/Shandong/10/2021 strain, and the clinical signs and body weights were evaluated every day. In addition, viremia, virus loads, and the expression of pro-inflammatory cytokines in mice brains and lungs were assessed at 1, 3, 5, and 7 days post infection (dpi). Our results demonstrated that mice in the EqHV-8 infected group displayed body weight loss, dyspnea signs, and viremia. The expression of interleukin (IL)-1ß, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-6 mRNA was increased in the brains and lungs of EqHV-8-infected mice than that in control group at 5 dpi and 7 dpi, and IL-12a expression was increased at 7 dpi. These data indicated that EqHV-8 elicited a strong cytokines response, caused neurogenic disease and respiratory signs in C57BL/6J mice, thus revealing the pathogenicity of EqHV-8.


Subject(s)
Cytokines , Viremia , Animals , Horses , Mice , Cytokines/metabolism , Mice, Inbred C57BL , Virulence , Tumor Necrosis Factor-alpha , Equidae , Interleukin-1beta
3.
Cell Mol Life Sci ; 80(1): 18, 2022 Dec 24.
Article in English | MEDLINE | ID: mdl-36564652

ABSTRACT

Glomerular diseases afflict millions of people and impose an enormous burden on public healthcare costs worldwide. Identification of potential therapeutic targets for preventing glomerular diseases is of considerable clinical importance. CHILKBP is a focal adhesion protein and modulates a wide array of biological functions. However, little is known about the role of CHILKBP in glomerular diseases. To investigate the function of CHILKBP in maintaining the structure and function of podocytes in a physiologic setting, a mouse model (CHILKBP cKO) was generated in which CHILKBP gene was conditionally deleted in podocytes using the Cre-LoxP system. Ablation of CHILKBP in podocytes resulted in massive proteinuria and kidney failure in mice. Histologically, typical podocyte injury including podocyte loss, foot process effacement, and glomerulosclerosis was observed in CHILKBP cKO mice. Mechanistically, we identified ZO-1 as a key junctional protein that interacted with CHILKBP. Loss of CHILKBP in podocytes exhibited a significant reduction of ZO-1 expression, leading to abnormal actin organization, aberrant slit diaphragm protein expression and compromised podocyte filtration capacity. Restoration of CHILKBP or ZO-1 in CHILKBP-deficient podocytes effectively alleviated podocyte injury induced by the loss of CHILKBP in vitro and in vivo. Finally, we showed the glomerular expression of CHILKBP and ZO-1 was decreased in patients with proteinuric kidney diseases. Our findings reveal a novel signaling pathway consisting of CHILKBP and ZO-1 that plays an essential role in maintaining podocyte homeostasis and suggest novel therapeutic approaches to alleviate glomerular diseases.


Subject(s)
Kidney Diseases , Podocytes , Mice , Animals , Podocytes/metabolism , Kidney Glomerulus/metabolism , Kidney Diseases/metabolism , Signal Transduction , Proteinuria/metabolism
4.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(6): 1487-9, 2012 Jun.
Article in Chinese | MEDLINE | ID: mdl-22870625

ABSTRACT

The emission spectrum line shift and vibrational temperature of the bright dot and dark dot discharges, which are observed in the argon and air dielectric barrier discharge at high temperature for the first time were measured and compared. The line shift of the spectral line of the Ar I (2P2-->1S5) is measured and the vibrational temperature was calculated using by the emission spectral lines of the N2 second positive band system (C3Pi(u)-->B3Pi(g)). The results show that the spectrum line shift of the bright dot discharge channel is larger than that of the dark dot channel, which indicates that the former has higher electron density compared to the latter, and the vibrational temperature of the dark dot discharge channel is higher than that of the bright dot discharge channel.

5.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2620-2, 2012 Oct.
Article in Chinese | MEDLINE | ID: mdl-23285851

ABSTRACT

The white-eye pattern, whose cell is composed of a bright dot surrounded by a closed hexagon, was observed in air/ argon dielectric barrier discharge. It was found that the center dot, the vertex of hexagon and the center of hexagon side in a cell have different brightness. By using optical emission spectra, the vibrational temperature in the center dot, the vertex of hexagon and the center of hexagon side was measured, respectively. The variations in the vibrational temperature at these three places as a function of the content of argon in gas mixture were also studied. The vibrational temperature was calculated by emission spectral lines of the N2 second positive band system (C3IIu --> B3IIg). The experimental results show that the vibrational temperature of the center dot, the vertex of hexagon and the center of hexagon side is in the ascending order and decreases with the increase in the content of argon in gas mixture.

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