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1.
Arch Virol ; 146(4): 685-96, 2001.
Article in English | MEDLINE | ID: mdl-11407396

ABSTRACT

Genomic properties of 62 field isolates of bovine viral diarrhea virus (BVDV) collected from 1974 to 1999 in Japan were investigated. The 5' untranslated region (UTR) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and the 244 to 247 base nucleotide sequences were determined. Serological properties were also characterized by the cross-neutralization test using antisera against the representative strain of the classified genotype. Using phylogenetic tree analysis, BVDV 1 was subdivided into two major clusters, BVDV-1a (29 isolates) and BVDV-1b (27 isolates). In group 1a, 3 differed from the other viruses, and were classified in a branch assigned as 1a'. However, 4 isolates (So CP/75, 190 CP, 190 NCP and KS86-1-NCP) could not be assigned to group 1a or 1b. In comparison with the published sequence data, KS86-1-NCP, 190 CP and 190 NCP were similar to the Southern Africa isolates that have recently been proposed as BVDV 1c. The 5' UTR sequence of So CP/75 was unique among those of BVDV 1, suggesting that the isolate should be classified into a new genotype. Only 2 out of 62 isolates collected in 1989 and 1990 were identified as BVDV 2. The results of the cross-neutralization test strongly supported these data, suggesting a close correlation between the 5' UTR sequence and the antigenicity of BVDV.


Subject(s)
Antibodies, Viral/immunology , Bovine Virus Diarrhea-Mucosal Disease/virology , Diarrhea Virus 1, Bovine Viral/genetics , Diarrhea Virus 1, Bovine Viral/immunology , Diarrhea Virus 2, Bovine Viral/genetics , Diarrhea Virus 2, Bovine Viral/immunology , 5' Untranslated Regions , Animals , Cattle , Cells, Cultured , Cross Reactions , Diarrhea Virus 1, Bovine Viral/isolation & purification , Diarrhea Virus 2, Bovine Viral/isolation & purification , Genome, Viral , Japan , Neutralization Tests , Phylogeny , Species Specificity
2.
J Vet Med Sci ; 54(6): 1137-43, 1992 Dec.
Article in English | MEDLINE | ID: mdl-1477164

ABSTRACT

For obtaining the preliminary data on the pathogenesis of sudden death in calves naturally heavily infected with Strongyloides papillosus, we monitored 8 Holstein calves experimentally infected with the larvae on electrocardiographic and pneumographic changes. Six calves died suddenly on days 11 to 17 after infection. Sinus tachycardia had been recorded continuously since 1 to 6 days before death. Heart rates increased gradually until death. Since 1 or 2 days before death, various patterns of tachyarrhythmia and bradyarrhythmia had been observed among patterns of sinus tachycardia. Arrhythmias included serious ventricular premature beat, paroxysmal ventricular tachycardia, complete atrioventricular block and so on. The terminal pattern observed suddenly in all of the cases was ventricular arrhythmias consisting of serial ventricular tachycardia, flutter and fibrillation, which were followed by respiratory arrest. Abnormal pneumograms were not obtained before the terminal ventricular fibrillation. Two of 8 calves recovered from the infection, only one of which showed sinus arrest and the second degree of atrioventricular block transiently. We concluded that calves heavily infected with the larvae died due to sudden cardiac arrest.


Subject(s)
Cattle Diseases/mortality , Death, Sudden, Cardiac/veterinary , Strongyloidiasis/veterinary , Animals , Cattle , Cattle Diseases/parasitology , Cattle Diseases/physiopathology , Electrocardiography/veterinary , Female , Male , Respiration , Strongyloidiasis/mortality , Strongyloidiasis/physiopathology , Tachycardia/parasitology , Tachycardia/veterinary
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