ABSTRACT
The possible use of high density lipoprotein (HDL) cholesterol, HDL phospholipids, apolipoproteins (APO) A-I and A-II as markers of alcohol abuse was studied in 78 intemperate drinkers. The mean value for each of these parameters was higher in drinkers than in control subjects. The most significant increase was observed in the plasma apo A-II levels (+45%). A composite index of gammaglutamyltranspeptidase (GGTP) and apo A-II was superior to GGTP alone in discriminating drinkers (+14%). Moreover, apo A-II assay is simple to perform.
Subject(s)
Alcoholism/blood , Apolipoproteins A/blood , Adult , Apolipoprotein A-I , Apolipoprotein A-II , Cholesterol, HDL/blood , Female , Humans , Lipoproteins, HDL/blood , Male , Middle Aged , Phospholipids/blood , gamma-Glutamyltransferase/bloodABSTRACT
Plasma lipids, lipoproteins and apolipoproteins A1 and B were determined in a reference population and in 273 patients who underwent coronary arteriography for evaluation of chest pain. The patients were divided into 3 groups according to arteriographic findings. The 102 patients in group A had normal coronary arteries; the 30 patients in group B had small vascular abnormalities but no significant narrowing; the 141 patients in group C had significant vascular lesions with greater than or equal to 50% narrowing. Total cholesterol and triglycerides were quantified using enzymatic methods. HDL cholesterol and phospholipids were enzymatically analyzed after phosphotungstate precipitation. Plasma lipoproteins were evaluated by quantitative electrophoresis based on densitometric scanning of the lipoprotein bands after separation and visualization by polyanion precipitation. Apoproteins A1 and B were measured by electroimmuno-diffusion assay using ready-for-use plates. Statistical evaluation of the data showed that group C patients had higher betalipoprotein and apo B levels and lower alphalipoprotein and apo A1 levels than subjects with normal coronary arteries. The predictive value of these results was established in relation to clinical findings. Plasma lipid measurements proved inadequate to separate the 3 groups. In contrast, the betalipoprotein/alphalipoprotein ratio and, still better, the apo A1/apo B ratio provided good discrimination. The results suggest that measurement of plasma apo A1 and apo B levels is the best means of assessing the risk of coronary artery disease.
Subject(s)
Apolipoproteins/blood , Coronary Disease/diagnosis , Lipoproteins/blood , Adult , Apolipoprotein A-I , Apolipoproteins B , Cholesterol/blood , Coronary Angiography , Coronary Disease/blood , Female , Humans , Hyperlipidemias/blood , Male , Middle Aged , Triglycerides/bloodSubject(s)
Apolipoproteins/blood , Apolipoproteins B , Coronary Disease/blood , Humans , Immunodiffusion/methodsABSTRACT
Triacylglycerides (TAG), total cholesterol (TC) and serum lipoproteins (SLP) were studied during the course of 12 cases of human malaria and one case of simian inui malaria. The erythrocytic schizogony induced quickly an increase of TAG, a decrease of TC and modifications of SLP such an increase of VLDL parallel with a decrease or a disappearance of HDL. These modifications, always clear-cut, were not correlated with the intensity of parasitaemia or the species of Plasmodium. They were transient but longer than those described in connection with experimental rodent malaria. The changes of HDL could be associated with defective triglyceride removal of the plasma compartment due to an inhibition of the lipoprotein lipase induced by the parasite.
Subject(s)
Lipoproteins/blood , Malaria/blood , Animals , Cholesterol/blood , Haplorhini , Humans , Lipoproteins, HDL/blood , Lipoproteins, VLDL/blood , Plasmodium falciparum , Plasmodium vivax , Triglycerides/bloodABSTRACT
An enzymatic and immunologic study of 18 patients with trichinosis leads to the following conclusions: The stage of muscular invasion in trichinosis is accompanied by a release of cellular enzymes representative of striated muscle fibres in nearly all the cases. This release can be observed by a study of the LDH iso-enzymes at a time when immunological techniques are not always significantly positive. The specific aspect of this phenomenon can be proposed with reservations since there does not exist any interference with other enzymatic systems such as the gamma-GT and furthermore no other evident cause of muscular lysis is present. The existence of a blood hypereosinophilia completes the biological picture. These early modifications of the enzymatic activities are most probably transient.