ABSTRACT
Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm
ABSTRACT
Background: Researches have been conducted in order to maintain the quality of the fresh semen which is diluted, refrigerated or frozen in liquid nitrogen for artificial insemination purposes in dogs. The semen biotechnology cooperates with the development of new formulations of types of extenders which minimize the death of the sperms due to thermal stress during temperature reduction of the refrigeration and freezing curves of the semen. The objective was to study the influence of the addition of vitamin E in types of extenders in the quality of the fresh, refrigerated and frozen semen in dogs of French Bulldog breed.Materials, Methods & Results: Semen samples by digital manipulation were performed on 5 adult dogs, French bulldog breed, five on each dog, totaling 25 ejaculated. The characteristics evaluated in fresh semen were: Volume (mL), color, aspect, concentration (x106/mL), sperm motility (%), vigor (1-5) and sperm morphology (%). For refrigerated and frozen semen, motility (%), vigor (1-5) and morphology (%) were analyzed. The ejaculated ones were fractionated in 4 equal parts and diluted in the ratio 1: 1 in the following extenders: 1 TRIS - Fructose Citric acid + 200 mM of vitamin E; 2 TRIS - Fructose Citric acid; 3 - coconut water (ACP-106®) + 200 mM of vitamin E; and 4 coconut water (ACP-106®). The four aliquots of semen, diluted in the four respective ex
ABSTRACT
Background: Researches have been conducted in order to maintain the quality of the fresh semen which is diluted, refrigerated or frozen in liquid nitrogen for artificial insemination purposes in dogs. The semen biotechnology cooperates with the development of new formulations of types of extenders which minimize the death of the sperms due to thermal stress during temperature reduction of the refrigeration and freezing curves of the semen. The objective was to study the influence of the addition of vitamin E in types of extenders in the quality of the fresh, refrigerated and frozen semen in dogs of French Bulldog breed.Materials, Methods & Results: Semen samples by digital manipulation were performed on 5 adult dogs, French bulldog breed, five on each dog, totaling 25 ejaculated. The characteristics evaluated in fresh semen were: Volume (mL), color, aspect, concentration (x106/mL), sperm motility (%), vigor (1-5) and sperm morphology (%). For refrigerated and frozen semen, motility (%), vigor (1-5) and morphology (%) were analyzed. The ejaculated ones were fractionated in 4 equal parts and diluted in the ratio 1: 1 in the following extenders: 1 TRIS - Fructose Citric acid + 200 mM of vitamin E; 2 TRIS - Fructose Citric acid; 3 - coconut water (ACP-106®) + 200 mM of vitamin E; and 4 coconut water (ACP-106®). The four aliquots of semen, diluted in the four respective ex
ABSTRACT
Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm
ABSTRACT
Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm
ABSTRACT
Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm
ABSTRACT
Background: Researches have been conducted in order to maintain the quality of the fresh semen which is diluted, refrigerated or frozen in liquid nitrogen for artificial insemination purposes in dogs. The semen biotechnology cooperates with the development of new formulations of types of extenders which minimize the death of the sperms due to thermal stress during temperature reduction of the refrigeration and freezing curves of the semen. The objective was to study the influence of the addition of vitamin E in types of extenders in the quality of the fresh, refrigerated and frozen semen in dogs of French Bulldog breed.Materials, Methods & Results: Semen samples by digital manipulation were performed on 5 adult dogs, French bulldog breed, five on each dog, totaling 25 ejaculated. The characteristics evaluated in fresh semen were: Volume (mL), color, aspect, concentration (x106/mL), sperm motility (%), vigor (1-5) and sperm morphology (%). For refrigerated and frozen semen, motility (%), vigor (1-5) and morphology (%) were analyzed. The ejaculated ones were fractionated in 4 equal parts and diluted in the ratio 1: 1 in the following extenders: 1 TRIS - Fructose Citric acid + 200 mM of vitamin E; 2 TRIS - Fructose Citric acid; 3 - coconut water (ACP-106®) + 200 mM of vitamin E; and 4 coconut water (ACP-106®). The four aliquots of semen, diluted in the four respective ex
ABSTRACT
Background: Researches have been conducted in order to maintain the quality of the fresh semen which is diluted, refrigerated or frozen in liquid nitrogen for artificial insemination purposes in dogs. The semen biotechnology cooperates with the development of new formulations of types of extenders which minimize the death of the sperms due to thermal stress during temperature reduction of the refrigeration and freezing curves of the semen. The objective was to study the influence of the addition of vitamin E in types of extenders in the quality of the fresh, refrigerated and frozen semen in dogs of French Bulldog breed.Materials, Methods & Results: Semen samples by digital manipulation were performed on 5 adult dogs, French bulldog breed, five on each dog, totaling 25 ejaculated. The characteristics evaluated in fresh semen were: Volume (mL), color, aspect, concentration (x106/mL), sperm motility (%), vigor (1-5) and sperm morphology (%). For refrigerated and frozen semen, motility (%), vigor (1-5) and morphology (%) were analyzed. The ejaculated ones were fractionated in 4 equal parts and diluted in the ratio 1: 1 in the following extenders: 1 TRIS - Fructose Citric acid + 200 mM of vitamin E; 2 TRIS - Fructose Citric acid; 3 - coconut water (ACP-106®) + 200 mM of vitamin E; and 4 coconut water (ACP-106®). The four aliquots of semen, diluted in the four respective ex
ABSTRACT
Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm
ABSTRACT
Background: Researches have been conducted in order to maintain the quality of the fresh semen which is diluted, refrigerated or frozen in liquid nitrogen for artificial insemination purposes in dogs. The semen biotechnology cooperates with the development of new formulations of types of extenders which minimize the death of the sperms due to thermal stress during temperature reduction of the refrigeration and freezing curves of the semen. The objective was to study the influence of the addition of vitamin E in types of extenders in the quality of the fresh, refrigerated and frozen semen in dogs of French Bulldog breed.Materials, Methods & Results: Semen samples by digital manipulation were performed on 5 adult dogs, French bulldog breed, five on each dog, totaling 25 ejaculated. The characteristics evaluated in fresh semen were: Volume (mL), color, aspect, concentration (x106/mL), sperm motility (%), vigor (1-5) and sperm morphology (%). For refrigerated and frozen semen, motility (%), vigor (1-5) and morphology (%) were analyzed. The ejaculated ones were fractionated in 4 equal parts and diluted in the ratio 1: 1 in the following extenders: 1 TRIS - Fructose Citric acid + 200 mM of vitamin E; 2 TRIS - Fructose Citric acid; 3 - coconut water (ACP-106®) + 200 mM of vitamin E; and 4 coconut water (ACP-106®). The four aliquots of semen, diluted in the four respective ex
ABSTRACT
Background: In Brazil, a higher percentage of abnormal sperm after high temperature and relative humidity observed a higher semen volume in the rainy. The semen quality may be significantly affected by environment, by fluctuations in temperature, humidity and photoperiod. The objective of this study was to evaluate the seasons influence (spring, summer, autumn and winter) on semen characteristics and testosterone levels in Nellore and Simmental bulls raised on field. Materials, Methods & Results: The city of Presidente Prudente-SP-Brazil, where the experiment was realize, presents latitude 2129`50``S, longitude 49º14`2``W and altitude of 475 meters. In 2008/2009, the climatic factors measured for Spring - SP; Summer - SU; Autumn A and the Winter - W were: average temperature (SP - 25.9; SU - 26.6; A - 21.9 and W - 21.1C), stroke (SP - 400.9; SU - 464.0; A - 721.3 and W - 142.6 hours), cumulative rainfall (SP - 291.8; SU - 925,0; A - 273.0; and W - 191.8 mm) and average relative humidity (SP - 65.9; SU - 71.7; A - 70.1 and W - 66.7%). Five Nellore and five Simmental bulls with 48-72 months old, extensively managed were evaluated for sexual soundness using physical and morphological characteristics of semen and testosterone serum levels. Sixteen semen samples by automatic electroejaculation were carried out in each of 10 bulls, totaling 160 samples. The experiment was con
Background: In Brazil, a higher percentage of abnormal sperm after high temperature and relative humidity observed a higher semen volume in the rainy. The semen quality may be significantly affected by environment, by fluctuations in temperature, humidity and photoperiod. The objective of this study was to evaluate the seasons influence (spring, summer, autumn and winter) on semen characteristics and testosterone levels in Nellore and Simmental bulls raised on field. Materials, Methods & Results: The city of Presidente Prudente-SP-Brazil, where the experiment was realize, presents latitude 2129`50``S, longitude 49º14`2``W and altitude of 475 meters. In 2008/2009, the climatic factors measured for Spring - SP; Summer - SU; Autumn A and the Winter - W were: average temperature (SP - 25.9; SU - 26.6; A - 21.9 and W - 21.1C), stroke (SP - 400.9; SU - 464.0; A - 721.3 and W - 142.6 hours), cumulative rainfall (SP - 291.8; SU - 925,0; A - 273.0; and W - 191.8 mm) and average relative humidity (SP - 65.9; SU - 71.7; A - 70.1 and W - 66.7%). Five Nellore and five Simmental bulls with 48-72 months old, extensively managed were evaluated for sexual soundness using physical and morphological characteristics of semen and testosterone serum levels. Sixteen semen samples by automatic electroejaculation were carried out in each of 10 bulls, totaling 160 samples. The experiment was con
ABSTRACT
Background: In Brazil, a higher percentage of abnormal sperm after high temperature and relative humidity observed a higher semen volume in the rainy. The semen quality may be significantly affected by environment, by fluctuations in temperature, humidity and photoperiod. The objective of this study was to evaluate the seasons influence (spring, summer, autumn and winter) on semen characteristics and testosterone levels in Nellore and Simmental bulls raised on field. Materials, Methods & Results: The city of Presidente Prudente-SP-Brazil, where the experiment was realize, presents latitude 2129`50``S, longitude 49º14`2``W and altitude of 475 meters. In 2008/2009, the climatic factors measured for Spring - SP; Summer - SU; Autumn A and the Winter - W were: average temperature (SP - 25.9; SU - 26.6; A - 21.9 and W - 21.1C), stroke (SP - 400.9; SU - 464.0; A - 721.3 and W - 142.6 hours), cumulative rainfall (SP - 291.8; SU - 925,0; A - 273.0; and W - 191.8 mm) and average relative humidity (SP - 65.9; SU - 71.7; A - 70.1 and W - 66.7%). Five Nellore and five Simmental bulls with 48-72 months old, extensively managed were evaluated for sexual soundness using physical and morphological characteristics of semen and testosterone serum levels. Sixteen semen samples by automatic electroejaculation were carried out in each of 10 bulls, totaling 160 samples. The experiment was con
Background: In Brazil, a higher percentage of abnormal sperm after high temperature and relative humidity observed a higher semen volume in the rainy. The semen quality may be significantly affected by environment, by fluctuations in temperature, humidity and photoperiod. The objective of this study was to evaluate the seasons influence (spring, summer, autumn and winter) on semen characteristics and testosterone levels in Nellore and Simmental bulls raised on field. Materials, Methods & Results: The city of Presidente Prudente-SP-Brazil, where the experiment was realize, presents latitude 2129`50``S, longitude 49º14`2``W and altitude of 475 meters. In 2008/2009, the climatic factors measured for Spring - SP; Summer - SU; Autumn A and the Winter - W were: average temperature (SP - 25.9; SU - 26.6; A - 21.9 and W - 21.1C), stroke (SP - 400.9; SU - 464.0; A - 721.3 and W - 142.6 hours), cumulative rainfall (SP - 291.8; SU - 925,0; A - 273.0; and W - 191.8 mm) and average relative humidity (SP - 65.9; SU - 71.7; A - 70.1 and W - 66.7%). Five Nellore and five Simmental bulls with 48-72 months old, extensively managed were evaluated for sexual soundness using physical and morphological characteristics of semen and testosterone serum levels. Sixteen semen samples by automatic electroejaculation were carried out in each of 10 bulls, totaling 160 samples. The experiment was con
ABSTRACT
O sucesso da criopreservação do sêmen depende da manutenção do potencial de fertilidade dos espermatozoides. Nos espermatozoides deve haver a preservação da integridade e funcionalidade de várias das suas estruturas. A fase de estabilização permite a saída de água dos espermatozoides por osmose. Este estudo tem o objetivo de comparar o efeito da refrigeração em refrigerador comercial (RC) e em caixa de transporte refrigerada (CTR) na viabilidade do sêmen congelado bovino diluído em três diferentes meios (A, B e C). Dez touros Nelore, Bos taurus indicus mantidos em central de inseminação artificial foram utilizados e as amostras de sêmen analisadas para checar a integridade das membranas plasmáticas e por meio da análise computadorizada (CASA). A fase de estabilização (5C/4 horas) foi realizada em RC e em CTR, sendo as amostras expostas ao vapor de nitrogênio durante 20 minutos e após mergulhadas no nitrogênio. A estatística foi feita com a análise de variância com nível de significância a 5%. No RC os parâmetros pós-descongelação para PM e ALH foram superiores (p 0,05) no meio B em relação ao C. Amostras que foram estabilizadas na CTR apresentaram parâmetros superiores (p 0,05) para PM e LIN nos meios A e B, em relação ao C. Os parâmetros BCF e STR foram superiores (p 0,05) no meio B em relação ao C. As amostras do meio B tiveram maior (p 0,05) PMI quando e
Sperm cryopreservation success depends upon the maintenance of spermatozoa fertility potential. Sperm cells must preserve both integrity and functionality of several cell structures. The stabilization phase must allow the exit of water from the sperm cells via osmosis. This study aimed to compare the effect of refrigeration in the commercial refrigerator (CR) and the transport/refrigeration box (TRB) upon the viability of frozen bull sperm diluted in three different extenders (A, B and C). Ten Nellore bulls, Bos taurus indicus maintained in Artificial Insemination Center were used and the spermatozoa samples was assessed for Plasma Membrane Integrity and CASA evaluation. The stabilization phase (5C/4 hours) was performed in the CR as well as in the TRB, and then samples were exposed to nitrogen vapor during 20 minutes and then plunged into nitrogen. The statistical analysis was done using the variance analysis and the significance level was set at 5%. In the CR the post-thawing parameters for PM and ALH were higher (p 0.05) in the extender A (glicine egg-yolk) and extender B (glicine egg-free) when compared with extender C (TRIS egg-yolk). As for BCF, STR and LIN, the parameters were higher (p 0.05) in extender B than in C. Samples that were stabilized in the TRB presented higher post-thawing parameters (p 0.05) for PM and LIN in extender A and extender B when c
ABSTRACT
O sucesso da criopreservação do sêmen depende da manutenção do potencial de fertilidade dos espermatozoides. Nos espermatozoides deve haver a preservação da integridade e funcionalidade de várias das suas estruturas. A fase de estabilização permite a saída de água dos espermatozoides por osmose. Este estudo tem o objetivo de comparar o efeito da refrigeração em refrigerador comercial (RC) e em caixa de transporte refrigerada (CTR) na viabilidade do sêmen congelado bovino diluído em três diferentes meios (A, B e C). Dez touros Nelore, Bos taurus indicus mantidos em central de inseminação artificial foram utilizados e as amostras de sêmen analisadas para checar a integridade das membranas plasmáticas e por meio da análise computadorizada (CASA). A fase de estabilização (5C/4 horas) foi realizada em RC e em CTR, sendo as amostras expostas ao vapor de nitrogênio durante 20 minutos e após mergulhadas no nitrogênio. A estatística foi feita com a análise de variância com nível de significância a 5%. No RC os parâmetros pós-descongelação para PM e ALH foram superiores (p 0,05) no meio B em relação ao C. Amostras que foram estabilizadas na CTR apresentaram parâmetros superiores (p 0,05) para PM e LIN nos meios A e B, em relação ao C. Os parâmetros BCF e STR foram superiores (p 0,05) no meio B em relação ao C. As amostras do meio B tiveram maior (p 0,05) PMI quando e
Sperm cryopreservation success depends upon the maintenance of spermatozoa fertility potential. Sperm cells must preserve both integrity and functionality of several cell structures. The stabilization phase must allow the exit of water from the sperm cells via osmosis. This study aimed to compare the effect of refrigeration in the commercial refrigerator (CR) and the transport/refrigeration box (TRB) upon the viability of frozen bull sperm diluted in three different extenders (A, B and C). Ten Nellore bulls, Bos taurus indicus maintained in Artificial Insemination Center were used and the spermatozoa samples was assessed for Plasma Membrane Integrity and CASA evaluation. The stabilization phase (5C/4 hours) was performed in the CR as well as in the TRB, and then samples were exposed to nitrogen vapor during 20 minutes and then plunged into nitrogen. The statistical analysis was done using the variance analysis and the significance level was set at 5%. In the CR the post-thawing parameters for PM and ALH were higher (p 0.05) in the extender A (glicine egg-yolk) and extender B (glicine egg-free) when compared with extender C (TRIS egg-yolk). As for BCF, STR and LIN, the parameters were higher (p 0.05) in extender B than in C. Samples that were stabilized in the TRB presented higher post-thawing parameters (p 0.05) for PM and LIN in extender A and extender B when c
ABSTRACT
Disruptions in sperm production include decreased sperm motility and increased of abnormal sperm. Seminal plasma appears to exert important effects on sperm function. The objective was to evaluate the dry and rainy season influence on the seminal characteristics and semen plasma proteins. Eleven bulls with ages ranging from 34 to 38 months were submitted each one to 12 semen collect with eletroejaculation 6 on dry season and 6 on rainy season with 14 days interval, totalizing 144 samples. Qualitative and quantitative semen characteristics were evaluated. Samples of semen were centrifuged (1.500 g / 15 minutes) and conditioned and stored (20ºC) until further processing. The proteins were extracted and quantified to electrophoresis performed. Variance analysis and Tukey test 5% was used. The semen vigor (P 0.01), minor defects and total defects (P 0.05) showed statistical difference between seasons, while the volume, motility and minor defects did not (P > 0.05). The number of bands occurred between 6 and 125 kDa. The molecular band of 26 kDa was present in 100% of bulls in rainy season. The molecular bands of 6, 9 and 125 kDa showed a high frequency in dry and rainy season. In conclusion, these results showed a band distribution variation throughout the season and the year seasons changed the semen quality with increase sperm vigor and reduction of abnormal sperm o
O desequilíbrio na espermatogênese inclui diminuição na motilidade e aumento de espermatozóides alterados. O plasma seminal exerce efeito na função espermática. O objetivo foi avaliar a influência das estações seca e chuvosa nas características do sêmen e proteínas do plasma seminal. Onze touros Bos taurus indicus com 34 a 38 meses de idade foram submetidos cada um a 12 colheitas de sêmen com eletroejaculador, 6 na estação seca e 6 na chuvosa, a cada 14 dias, totalizando 144 amostras. Características qualitativas e quantitativas foram avaliadas, sendo as amostras centrifugadas (1.500 g / 15 min) e estocadas (-20ºC). As proteínas foram extraídas e quantificadas para as eletroforeses. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5%. Houve diferença entre estações para o vigor (P 0,01), defeitos menores e totais (P 0,05); e não para o volume, motilidade e defeitos maiores. A ocorrência do número de bandas esteve entre 6 e 125 kDa. A banda de 26 kDa esteve presente em 100% dos touros na estação chuvosa. As bandas de 6, 9 e 125 kDa mostraram alta freqüência nas estações seca e chuvosa. Conclui-se que os resultados demonstraram variação na distribuição de bandas nas estações seca e chuvosa; e que as estações do ano alteram a qualidade do sêmen com melhoria do vigor e redução das patologias espermáticas na estação seca.
ABSTRACT
The present study aims to compare the effects of lower doses of cloprostenol intramuscular (IM) or into vulvar submucosa (IVS) on estrus induction and pregnancy rate in Nelore cows. A total of 100 cycling Nelore cows with body condition score (BCS 3.5) 1 to 5 scale (Radostitis; Blood, 1986) and 170±11 days postpartum. Females were randomly divided in 5 groups (N=20) G1 to G5 and treated with cloprostenol (CiosinÒ) on day 0 (D0) and on day 11 (D11) if not detected in estrus. Cows were injected with 500mg IM (G1), 250mg IM (G2), 125mg IM (G3), 250mg IVS (G4) and 125mg IVS (G5). Estrus was observed twice a day and the females artificially inseminated 12 hours after heat detection. There was no statistical difference (P > 0.80) between groups in the estrus induction (first injection to estrus interval): 16/20 - 96.00 hours (G1), 13/20 - 90.42 hours (G2), 10/20 - 84.45 hours (G3), 15/20 87.86 hours (G4), 12/20 - 81.25 hours (G5) and second injection (P > 0.10): 4/20 67.50 hours (G1), 7/20 85.50 hours (G2), 10/20 - 57.00 hours (G3), 5/20 70.60 hours (G4), 8/20 60.00 hours (G5). There was no statistical difference (0.65ns) between groups in the pregnancy rates: 40% (G1), 45% (G2), 50% (G3), 40% (G4), 40% (G5). The results demonstrate that the treatments with lower doses of cloprostenol intramuscular or into vulvar submucosa may be used to induce heat with similar pregn
O presente estudo teve o objetivo de comparar os efeitos de baixas doses de cloprostenol na via intramuscular (IM) ou submucosavulvar (SMV) na detecção do estro e taxas de prenhez em vacas Nelore. Utilizou-se 100 vacas Nelore cíclicas com condição de escore corporal (CEC 3,5) na escala de 1 a 5 (Radostitis; Blood, 1986) com 170±11 dias pós-parto. As fêmeas foram divididas aleatoriamente em cinco grupos (N=20) G1 a G5 e tratadas com cloprostenol (CiosinÒ) no dia zero (D0) e no dia 11 (D11) se não detectada em estro. As vacas foram injetadas com 500mg IM (G1), 250mg IM (G2), 125mg IM (G3), 250mg SMV (G4) e 125mg SMV (G5). O estro foi observado duas vezes por dia e as fêmeas inseminadas artificialmente 12 horas após a detecção do mesmo. Não houve diferença estatística entre grupos (P > 0,80) na indução do estro (intervalo em horas: primeira injeção estro): 16/20 96,00 h (G1), 13/20 90,42 h (G2), 10/20 84,45 h (G3), 15/20 87,86 h (G4), 12/20 81,25 h (G5) e para a segunda injeção (P > 0,10): 4/20 67,50 h (G1), 7/20 85,50 h (G2), 10/20 57,00 h (G3), 5/20 70,60 h (G4), 8/20 60,00 h (G5). Não houve diferença significativa (0,65ns) entre grupos para as taxas de prenhez: 40% (G1), 45% (G2), 50% (G3), 40% (G4) e 40% (G5). Os resultados demonstram que os tratamentos com baixas doses de cloprostenol intramuscular ou submucosavulvar podem ser usados na indução do
ABSTRACT
The purpose of this study was to investigate the nutraceutic (PromaterÒ) influence on semen, body weight, eritrogram and hormones testosterone and cortisol in Nelore young bulls. Twenty Nelore bulls were utilized, aging between 30 and 36 months, divided in group 1 (G1)=10 (control) and group 2 (G2)=10 (nutraceutic group - PromaterÒ: 20 mL/animal/day, during 70 days). Corporal weight, blood samples to eritrogram, testosterone and cortisol were obtained in days zero (D0), 35 (D35) and 70 (D70) in two groups. There was difference (P 0.01) among days collected (0, 35 and 70) with means of 424.30 kg, 480.70 kg and 502.60 kg, respectively, but there was not difference between groups. There was not difference among groups to semen, eritrogram, cortisol (G1 = 3,19 µg/dL; G2 = 2,91 µg/dL) and testosterone (G1 = 274,48 ng/dL; G2 = 509,65 ng/dL). The results exposed allow concluding that were not observed significative effect of the nutraceutic upon evaluated parameters: body weight, semen, hormonal profile and eritrogram.
O presente trabalho teve o objetivo de estudar a influência de um nutracêutico (PromaterÒ) no sêmen, peso corpóreo, eritrograma e nos hormônios testosterona e cortisol, em touros jovens da raça Nelore. Foram utilizados 20 touros da raça Nelore, com idades entre 30 e 36 meses, divididos em grupo 1 (G1)=10 (controle) e grupo 2 (G2)=10 (grupo nutracêutico (PromaterÒ): 20 mL/animal/dia, durante 70 dias). Peso corpóreo, amostras de sangue para análises do eritrograma, testosterona e cortisol; foram obtidos nos dias zero (D0), 35 (D35) e 70 (D70) nos dois grupos. Com relação ao peso, houve diferença (P 0,01) entre dias (0, 35 e 70) de colheita com médias de 424,30 kg, 480,70 kg e 502,60 kg, respectivamente, sem diferença significativa entre grupos. Não houve diferença (P > 0,05) entre grupos para o peso corpóreo, eritrograma; quadro espermático, cortisol (G1 = 3,19 µg/dL e G2 = 2,91 µg/dL) e testosterona (G1 = 247,48 ng/dL e G2 = 509,65 ng/dL). Conclui-se que o nutracêutico não influenciou nos níveis séricos de testosterona, cortisol e ganho de peso. Os parâmetros sanguíneos e do sêmen não sofreram alterações devido ao nutracêutico.
ABSTRACT
The objective of this experiment was to evaluate the effect of the probiotic Proenzime® on scrotal perimeter and spermiogram in young Tabapua bulls. Two samples of semen from 21 Tabapua bulls, aging 30 months, were collected in day zero (D0) and 120 (D120) by electroejaculation, totalizing 42 samples. The bulls were divided in control group (CG) with 10 animals and trated group (TG) with 11 animals. The bulls of TG received 4g of probiotic/animal/day, during 120 days. There was difference (p
Neste experimento avaliou-se o efeito do probiótico Proenzime® no perímetro escrotal e espermiograma em touros jovens Tabapuã. Duas colheitas de sêmen de 21 touros Tabapuã, com trinta meses de idade, foram realizadas nos dias zero (D0) e 120 (D120) por eletroejaculação, totalizando 42 amostras. Dividiram-se os touros em grupo-controle (GC) com dez animais e grupo tratado (GT) com onze animais. Os touros do GT receberam 4g de probiótico/animal/dia, durante 120 dias. Houve diferença (p
ABSTRACT
The study of ovarian morphometry is directly related to its practical applications to interpret the examination of the reproductive tract of the cow. The objective of this work was to study the morphometry and hormonal correlations in ovaries collected in slaughterhouse between 7 and 14 days post estrus in no pregnant zebu cows. One hundred fourteen pairs of ovaries were collected from slaughterhouse, measured in thickness, length, width and volume, diameter and volume of the follicle, diameter and area of the corpus luteum. Significative differences were observed for widths of (1.95 cm and 1.83 cm) and volume of (7.26 mL and 6.23 mL) of left and right ovaries respectively. For size and volume of follicles, and diameter and area of the corpus luteum, there was no significative difference between the sides. There was correlation (p
O estudo da morfometria ovariana está diretamente ligado as suas aplicações práticas, na realização e interpretação dos achados dos exames ginecológicos em vacas. O objetivo do presente trabalho foi estudar as correlações morfométricas e hormonais de 114 pares de ovários de vacas zebus não prenhes coletados em abatedouro entre os dias sete e quatorze do ciclo estral, procedendo-se a sua mensuração quanto a espessura, comprimento, largura e volume; diâmetro e volume do folículo, diâmetro e área do corpo lúteo, e concentrações de progesterona e cortisol. Observou-se diferença significativa para largura (1,95 cm e 1,83 cm) e volume (7,26 mL e 6,23 mL) do ovário esquerdo e direito, respectivamente. Houve correlação positiva (p
ABSTRACT
The study of ovarian morphometry is directly related to its practical applications to interpret the examination of the reproductive tract of the cow. The objective of this work was to study the morphometry and hormonal correlations in ovaries collected in slaughterhouse between 7 and 14 days post estrus in no pregnant zebu cows. One hundred fourteen pairs of ovaries were collected from slaughterhouse, measured in thickness, length, width and volume, diameter and volume of the follicle, diameter and area of the corpus luteum. Significative differences were observed for widths of (1.95 cm and 1.83 cm) and volume of (7.26 mL and 6.23 mL) of left and right ovaries respectively. For size and volume of follicles, and diameter and area of the corpus luteum, there was no significative difference between the sides. There was correlation (p
O estudo da morfometria ovariana está diretamente ligado as suas aplicações práticas, na realização e interpretação dos achados dos exames ginecológicos em vacas. O objetivo do presente trabalho foi estudar as correlações morfométricas e hormonais de 114 pares de ovários de vacas zebus não prenhes coletados em abatedouro entre os dias sete e quatorze do ciclo estral, procedendo-se a sua mensuração quanto a espessura, comprimento, largura e volume; diâmetro e volume do folículo, diâmetro e área do corpo lúteo, e concentrações de progesterona e cortisol. Observou-se diferença significativa para largura (1,95 cm e 1,83 cm) e volume (7,26 mL e 6,23 mL) do ovário esquerdo e direito, respectivamente. Houve correlação positiva (p