LAP2alpha maintains a mobile and low assembly state of A-type lamins in the nuclear interior.

Lamins form stable filaments at the nuclear periphery in metazoans. Unlike B-type lamins, lamins A and C localize also in the nuclear interior, where they interact with lamin-associated polypeptide 2 alpha (LAP2α). Using antibody labeling, we previously observed a depletion of nucleoplasmic A-type lamins in mouse cells lacking LAP2α. Here, we show that loss of LAP2α actually causes formation of larger, biochemically stable lamin A/C structures in the nuclear interior that are inaccessible to lamin A/C antibodies. While nucleoplasmic lamin A forms from newly expressed pre-lamin A during processing and from soluble mitotic lamins in a LAP2α-independent manner, binding of LAP2α to lamin A/C during interphase inhibits formation of higher order structures, keeping nucleoplasmic lamin A/C in a mobile state independent of lamin A/C S22 phosphorylation. We propose that LAP2α is essential to maintain a mobile lamin A/C pool in the nuclear interior, which is required for proper nuclear functions.

Exploring the Relationship of a Gamified Assessment with Performance.

Our study explores the validity of a game-based assessment method assessing candidates' soft skills. Using self-reported measures of performance, (job performance, Organizational Citizenship Behaviors (OCBs), and Great Point Average (GPA), we examined the criterion-related and incremental validity of a game-based assessment, above and beyond the effect of cognitive ability and personality. Our findings indicate that a game-based assessment measuring soft skills (adaptability, flexibility, resilience and decision making) can predict self-reported job and academic performance. Moreover, a game-based assessment can predict academic performance above and beyond personality and cognitive ability tests. The effectiveness of gamification in personnel selection is discussed along with research and practical implications introducing recruiters and HR professionals to an innovative selection technique.

Exploring the relationship of a gamified assessment with performance

Our study explores the validity of a game-based assessment method assessing candidates' soft skills. Using self-reported measures of performance, (job performance, Organizational Citizenship Behaviors (OCBs), and Great Point Average (GPA), we examined the criterion-related and incremental validity of a game-based assessment, above and beyond the effect of cognitive ability and personality. Our findings indicate that a game-based assessment measuring soft skills (adaptability, flexibility, resilience and decision making) can predict self-reported job and academic performance. Moreover, a game-based assessment can predict academic performance above and beyond personality and cognitive ability tests. The effectiveness of gamification in personnel selection is discussed along with research and practical implications introducing recruiters and HR professionals to an innovative selection technique

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Fairness reactions to the employment interview / Las reacciones de equidad a la entrevista de trabajo

The current research explores applicants' reactions to the employment interview and how these are associated with core self-evaluations and proactivity along with perceptions of the interviewer and interview's justice perceptions of post-interview outcomes (behavioral intentions, job attractiveness, and organizational attractiveness). We also explored the role of perceived organizational support (POS) in these relationships. We employ a cross-sectional approach, with the participation of 238 actual job applicants, using a survey methodology. We demonstrated the important role of core self-evaluations in fairness perceptions of the interview, along with the significant role of some interview characteristics, more importantly informativeness and personableness. Finally, applicants' perceptions of the employer were also strongly associated with interview's justice perceptions and post-interview outcomes. This is one of the first and very few studies exploring this topic in a non-English culture (in Greece), with actual job applicants, not students

La presente investigación explora las reacciones de los solicitantes a la entrevista de empleo y cómo dichas reacciones se asocian a las autoevaluaciones fundamentales y a la proactividad, junto con las percepciones del entrevistador y las percepciones de justicia de la entrevista de los resultados post-entrevista (intenciones conductuales, atractivo del puesto y atractivo de la organización). También exploramos el rol del apoyo organizacional percibido (POS) en estas interrelaciones. Empleamos un enfoque transversal, con la participación de 238 solicitantes reales del puesto, usando una metodología de encuestas. Demostramos el importante rol de las autoevaluaciones fundamentales en las percepciones de justicia de la entrevista, junto con el rol significativo de algunas características de la entrevista, de modo particularmente importante la capacidad informativa y la amabilidad. Finalmente, las percepciones de los solicitantes sobre el empleador estuvieron también fuertemente asociadas con las percepciones de justicia de la entrevista y con los resultados post-entrevista. Este es uno de los primeros y escasos estudios que exploran este tema en una cultura no inglesa (en Grecia), con solicitantes reales del puesto y no con estudiantes

Nucleoplasmic lamins define growth-regulating functions of lamina-associated polypeptide 2α in progeria cells.

A-type lamins are components of the peripheral nuclear lamina but also localize in the nuclear interior in a complex with lamina-associated polypeptide (LAP) 2α. Loss of LAP2α and nucleoplasmic lamins in wild-type cells increases cell proliferation, but in cells expressing progerin (a mutant lamin A that causes Hutchinson-Gilford progeria syndrome), low LAP2α levels result in proliferation defects. Here, the aim was to understand the molecular mechanism governing how relative levels of LAP2α, progerin and nucleoplasmic lamins affect cell proliferation. Cells from progeria patients and inducible progerin-expressing cells expressing low levels of progerin proliferate faster than wild-type or lamin A-expressing control cells, and ectopic expression of LAP2α impairs proliferation. In contrast, cells expressing high levels of progerin and lacking lamins in the nuclear interior proliferate more slowly, and ectopic LAP2α expression enhances proliferation. However, simultaneous expression of LAP2α and wild-type lamin A or an assembly-deficient lamin A mutant restored the nucleoplasmic lamin A pool in these cells and abolished the growth-promoting effect of LAP2α. Our data show that LAP2α promotes or inhibits proliferation of progeria cells depending on the level of A-type lamins in the nuclear interior.This article has an associated First Person interview with the first author of the paper.

Stage-Specific Changes in Plasmodium Metabolism Required for Differentiation and Adaptation to Different Host and Vector Environments.

Malaria parasites (Plasmodium spp.) encounter markedly different (nutritional) environments during their complex life cycles in the mosquito and human hosts. Adaptation to these different host niches is associated with a dramatic rewiring of metabolism, from a highly glycolytic metabolism in the asexual blood stages to increased dependence on tricarboxylic acid (TCA) metabolism in mosquito stages. Here we have used stable isotope labelling, targeted metabolomics and reverse genetics to map stage-specific changes in Plasmodium berghei carbon metabolism and determine the functional significance of these changes on parasite survival in the blood and mosquito stages. We show that glutamine serves as the predominant input into TCA metabolism in both asexual and sexual blood stages and is important for complete male gametogenesis. Glutamine catabolism, as well as key reactions in intermediary metabolism and CoA synthesis are also essential for ookinete to oocyst transition in the mosquito. These data extend our knowledge of Plasmodium metabolism and point towards possible targets for transmission-blocking intervention strategies. Furthermore, they highlight significant metabolic differences between Plasmodium species which are not easily anticipated based on genomics or transcriptomics studies and underline the importance of integration of metabolomics data with other platforms in order to better inform drug discovery and design.