Cbfa1 expression in vascular smooth muscle cells may be elevated by increased nitric oxide/iNOS.

INTRODUCTION: Vascular calcification is a common complication of chronic kidney disease. Osteoblast differentiation factor (Cbfa1) is present in histologic sections of arteries from patients with end-stage renal disease. Vascular smooth muscle cells (VSMC) can dedifferentiate to osteoblast-like cells, possibly by up-regulation of Cbfa1. There is evidence that the production of nitric oxide (NO) may have an important role in the regulation of osteoblast metabolism. The aim of this study is to evaluate whether increased NO/iNOS expression causes an increase in cbfa1 expression in VSMC. METHODS: VSMC were obtained from renal artery of Wistar male rats, treated for 72 hours with lipopolysaccharide (LPS), ß-glycerophosphate (BGF), a donor of phosphate and aminoguanidine (AG), an inhibitor of iNOS, in the following groups: CTL (control), LPS, BGF, LPS + BGF, and LPS + AG. NO synthesis was determined by chemiluminescence. Cbfa1 and iNOS mRNA expressions were analyzed by RT-PCR, Cbfa1 protein expression by immunohistochemistry and cellular viability by acridine orange. RESULTS: Cbfa1 and iNOS mRNA expressions were higher in LPS and LPS+ BGF vs CTL (p < 0.05), and they were lower in LPS+AG vs LPS (p < 0.05). The Cbfa1 in the groups LPS and LPS+BGF also resulted in a higher value compared to CTL (p < 0.05), and in LPS+AG it was lower compared to LPS (p < 0.05). NO was higher in LPS and LPS+BGF compared to CTL group (p < 0.05) and lower in LPS + AG compared to LPS group (p < 0.05). Cellular viability showed no statistical difference among groups. CONCLUSION: This study showed that increased NO/iNOS expression causes an increase in cbfa1 expression in VSMC.

Isotonic sports drink promotes rehydration and decreases proteinuria following karate training / Suplemento hidroeletrolítico favorece reidratação e diminui proteinúria pós-treino em atletas karate

Abstract Introduction: Adequate hydration status in the sport is essential for good health, yet the relationship between hydration, proteinuria and sports is little studied. Objective: To analyze the influence of an isotonic sports drink as rehydration strategy on the hydration status and proteinuria after karate training. Methods: Ten athletes participated in this study. In the first session of standard training, called observation training session (STO), the athletes hydrated themselves according to their habits, and in the second session of standard training, called nutritional intervention training session (STIN), an ideal practice of hydration protocol was followed, using an isotonic sports drink as a rehydration liquid during the training. The hydration status was verified by monitoring the body weight before and after training, the urine specific gravity pre-and post-training and the urine volume post-training. To observe the influence of practice of hydration on the renal function post exercise proteinuria was measured. Results: We observed a statistically significant difference in urine density between the samples pre- and post-exercise only on STIN (p = 0.047). When we compare the sessions, there was a lower variation in body weight (p = 0.011) and higher urinary volume (p < 0.001), on nutritional intervention training. In STO, there was a higher percentage of athletes who showed proteinuria (70%) compared to the STIN (50%) in the urine sample after training. Conclusion: The use of isotonic sports drink as practice of hydration by karate athletes promoted rehydration during one session of training and reduce post-training proteinuria.

Resumo Introdução: Um adequado estado de hidratação durante a atividade esportiva é essencial para a manutenção da boa saúde, porém a relação entre hidratação, proteinúria e esportes é pouco estudada. Objetivo: analisar a influência de suplemento hidroeletrolítico (bebida esportiva isotônica) como estratégia de reidratação sobre o estado de hidratação e proteinúria após treino de Karate. Métodos: Dez atletas participaram deste estudo. Na primeira sessão de treino padronizado, denominada sessão de treino de observação (STO), os atletas se reidrataram segundo seus hábitos; na segunda sessão de treino padronizado, denominada sessão de treino de intervenção nutricional (STIN), foi seguido um protocolo ideal de prática de hidratação, utilizando-se de suplemento hidroeletrolítico como líquido reidratante durante o treino. O estado de hidratação foi verificado pelo monitoramento do peso corporal antes e após o treino, pela gravidade específica da urina pré e pós-treino e pelo volume urinário pós-treino. De forma a observar a influência da prática de hidratação sobre a função renal, a proteinúria pós-exercício foi medida. Resultados: Observou-se uma diferença estatisticamente significativa na densidade urinária entre as amostras coletadas pré e pós-exercício apenas na STIN (p = 0,047). Quando comparados entre sessões, houve menor variação ponderal (p = 0,011) e maior volume urinário (p < 0,001) no treino com intervenção nutricional. Na STO, houve um percentual mais elevado de atletas que apresentaram proteinúria (70%) em comparação a STIN (50%) na amostra de urina coletada após o treino. Conclusão: O uso de suplemento hidroeletrolitico como prática de hidratação por atletas de karate favoreceu a reidratação durante uma sessão de treino e reduziu a proteinúria pós-treino.

Isotonic sports drink promotes rehydration and decreases proteinuria following karate training.

INTRODUCTION: Adequate hydration status in the sport is essential for good health, yet the relationship between hydration, proteinuria and sports is little studied. OBJECTIVE: To analyze the influence of an isotonic sports drink as rehydration strategy on the hydration status and proteinuria after karate training. METHODS: Ten athletes participated in this study. In the first session of standard training, called observation training session (STO), the athletes hydrated themselves according to their habits, and in the second session of standard training, called nutritional intervention training session (STIN), an ideal practice of hydration protocol was followed, using an isotonic sports drink as a rehydration liquid during the training. The hydration status was verified by monitoring the body weight before and after training, the urine specific gravity pre-and post-training and the urine volume post-training. To observe the influence of practice of hydration on the renal function post exercise proteinuria was measured. RESULTS: We observed a statistically significant difference in urine density between the samples pre- and post-exercise only on STIN (p = 0.047). When we compare the sessions, there was a lower variation in body weight (p = 0.011) and higher urinary volume (p < 0.001), on nutritional intervention training. In STO, there was a higher percentage of athletes who showed proteinuria (70%) compared to the STIN (50%) in the urine sample after training. CONCLUSION: The use of isotonic sports drink as practice of hydration by karate athletes promoted rehydration during one session of training and reduce post-training proteinuria.