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1.
Izv Akad Nauk Ser Biol ; (6): 693-703, 2001.
Article in Russian | MEDLINE | ID: mdl-15926335

ABSTRACT

This paper describes our study on the regeneration of hemopoietic and stromal components of bone marrow after mechanically emptying the medullar cavity of the guinea pig tibia. The intensity of hemopoiesis was determined from the number of hemopoietic cells, while the concentration and total number of stromal precursor cells were used to estimate the ability of the bone marrow to produce stromal structures, including its ability to restore a specific microenvironment. We found that there was no direct correlation between the recovery characteristics of hemopoietic and stromal cells. An increase in the population size of stromal precursor cells takes place early after curettage, and stromal fibroblasts become phosphatase-positive according to Gomori, which is characteristic of osteogenic tissue. We have also demonstrated that curettage of 3-5 tubular bones results in the growth of this cell population in the bone marrow of nonoperated bones and even in the spleen, which in guinea pigs participates only in lymphopoiesis.


Subject(s)
Bone Marrow Cells/physiology , Hematopoietic System/cytology , Lymphatic System/cytology , Regeneration , Stem Cells/physiology , Animals , Cell Differentiation , Clone Cells/physiology , Female , Guinea Pigs , Hematopoiesis/physiology , Hematopoietic System/physiology , Lymphatic System/physiology , Male , Stem Cells/cytology , Stromal Cells/physiology
2.
Article in Russian | MEDLINE | ID: mdl-9783387

ABSTRACT

The data on changes in the number, as well as cultural and biological properties of S. typhi and free-living T. pyriformis in the course of their joint cultivation at 2 degrees C and 25 degrees C are presented. As suggested on the basis of the results of this experiment, the interaction of S. typhi with T. pyriformis may facilitate the preservation of the bacteria in the environment and be a stage in their migration along trophic chains.


Subject(s)
Salmonella typhi/growth & development , Tetrahymena pyriformis/growth & development , Animals , Coculture Techniques/methods , Coculture Techniques/statistics & numerical data , Colony Count, Microbial , Culture Media , Salmonella typhi/pathogenicity , Temperature
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