ABSTRACT
The effect of arginine-vasotocin (50 ng ml-1) on the in vitro production of progesterone, testosterone, and oestradiol by isolated cow and chicken ovarian follicles and mouse ovaries was studied. This peptide (1) enhanced the release of progesterone into the medium by the ovarian tissues of all three species of animal; (2) inhibited the production of oestradiol by mouse and cow ovaries; (3) had no significant effect on testosterone secretion in any of the species studied. The results obtained indicate that arginine-vasotocin directly influences steroid production in mammalian and avian gonads.
Subject(s)
Ovary/drug effects , Steroids/biosynthesis , Vasotocin/pharmacology , Animals , Cattle , Chickens , Estradiol/biosynthesis , Female , In Vitro Techniques , Mice , Ovary/metabolism , Pregnancy , Progesterone/biosynthesis , Testosterone/biosynthesisABSTRACT
Prostaglandin of the F2-alpha group (estrophan) led to a sharp decrease in serum progesterone concentration of 90-96% of Edibai female sheep eliminating the blocking hypothalamic center effect of the endogenous steroid hormone. The resultant conclusion was a possibility of artificial insemination of the above number of female sheep without finding out signs of wish in them. Their fertility from the first frontal insemination was not below 62-70%.
