ABSTRACT
To increase the efficiency of interpretation of mast cell's contribution to the state of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. Developed method of combining immunomorphological and histochemical staining protocols contributes to the most objective detection of the integral level of tryptase expression in the intraorgan population of the skin mast cells. Novel technique for tryptase detection expands the possibilities of morphological analysis, provides researchers with additional data on the structure of the mast cell population and helps visualize the processing and cytological features and structural targets of tryptase during the development of adaptive and pathological reactions. Objective determination of the tryptase profile for organ-specific mast cell populations is in great demand in clinical practice for the interpretation of pathological processes, including inflammation and oncogenesis.
Subject(s)
Gene Expression Regulation, Enzymologic , Mast Cells , Skin , Staining and Labeling , Tryptases/biosynthesis , Animals , Male , Mast Cells/cytology , Mast Cells/enzymology , Organ Specificity , Rats , Rats, Wistar , Skin/cytology , Skin/enzymologyABSTRACT
The authors made an analysis of their first 100 transplantations of cadaveric liver made at the period from 1998 through 2011. Postoperative complications and long-term results of transplantations at the period to 13 years are described. Cumulative survival up to 12 months was 91%, to 36 months--83%. Retransplantation of the liver was performed on 5 patients, 2 of them being successful.
Subject(s)
End Stage Liver Disease/surgery , Liver Transplantation , Postoperative Complications/prevention & control , Tissue and Organ Harvesting , Adult , Female , Humans , Immunosuppression Therapy/methods , Kaplan-Meier Estimate , Liver Transplantation/adverse effects , Liver Transplantation/methods , Liver Transplantation/mortality , Male , Middle Aged , Monitoring, Immunologic , Patient Selection , Reoperation , Tissue and Organ Harvesting/methods , Tissue and Organ Harvesting/trends , Treatment OutcomeSubject(s)
Catheterization/methods , Common Bile Duct , Constriction, Pathologic , Drainage/methods , Liver Transplantation/adverse effects , Postoperative Complications/surgery , Anastomosis, Surgical/adverse effects , Anastomosis, Surgical/methods , Cholangiography , Common Bile Duct/pathology , Common Bile Duct/physiopathology , Common Bile Duct/surgery , Constriction, Pathologic/etiology , Constriction, Pathologic/physiopathology , Constriction, Pathologic/surgery , Female , Humans , Liver Cirrhosis/surgery , Liver Transplantation/methods , Stents , Treatment Outcome , Young AdultABSTRACT
The novel metalloporphyrins (M=HH, Fe, Mn, Co, Cu, Zn) bearing 2,6-di-tert-butylphenol pendants as antioxidant substituents, and a long chain hydrocarbon palmitoyl group have been synthesized. The oxidation of compounds by PbO2 leads to the formation of the corresponding 2,6-di-tert-butylphenoxyl radicals studied by EPR. The activity of porphyrins in lipid peroxidation has been examined using (1) in vitro lipid peroxidation induced by tert-butylhydroperoxide in respiring rat liver mitochondria, (2) in vitro lipid peroxidation in liver homogenates of Wistar strain rats, and (3) a model process of peroxidation of (Z)-octadec-9-enic (oleic) acid as a structural fragment of lipids. The activity of these compounds depends dramatically on the nature of metal and might be changed from antioxidative (M=HH, Mn, Cu, Zn) to indifferent (M=Co), and to pro-oxidative one (M=Fe). The anti- or pro-oxidative action of these compounds may be derived from the concurrence between the involvement of 2,6-di-tert-butylphenol pendants acting as radical scavengers and redox active metal center promoting oxidation processes. The results of this study suggest that the polytopic compounds combining in one molecule 2,6-di-tert-butylphenol pendants, metalloporphyrin moiety, and a palmitoyl group, are membrane active compounds and might be studied in an effort to find novel pharmaceutical agents.
Subject(s)
Antioxidants/chemical synthesis , Antioxidants/pharmacology , Metalloporphyrins/chemical synthesis , Metalloporphyrins/pharmacology , Palmitic Acids/chemistry , Phenols/chemistry , Animals , Cobalt/chemistry , Copper/chemistry , Electron Spin Resonance Spectroscopy , Iron/chemistry , Lipid Peroxidation/drug effects , Manganese/chemistry , Mitochondrial Membranes/drug effects , Oleic Acid/chemistry , Palmitic Acids/pharmacology , Rats , Rats, Wistar , Zinc/chemistryABSTRACT
New medicinal plant preparations of polyphenol nature, representing the derivatives of bioflavonoids (piflamin) and ellagotannins (altan and ellagic acid) were experimentally studied. The drugs exhibited antioxidant properties, which were manifested by inhibition of a pathological lipid peroxidation, restoration of the functional activity of the antioxidant system components, and stabilization of the hepatocyte membranes.
Subject(s)
Antioxidants/pharmacology , Ellagic Acid/pharmacology , Flavonoids/pharmacology , Tannins/pharmacology , Animals , Carbon Tetrachloride Poisoning/metabolism , Cell Membrane , Chemical and Drug Induced Liver Injury/metabolism , Female , Hepatocytes/drug effects , Hepatocytes/ultrastructure , Lipid Peroxidation/drug effects , Liver/drug effects , Liver/metabolism , Liver/ultrastructure , Male , Mice , RatsABSTRACT
The hepatoprotective properties of the flavonoid preparation piflamine of field-peas grass were studied on a model of experimental paracetamol liver damage. Piflamine was found to normalize the parameters of carbohydrate, protein, and lipid metabolism, increase the activity of the antioxidant system, and restore the processes of bile production and bile secretion. The drug is prospective due to its quite cheap and available source of raw materials.