ABSTRACT
A new alcohol oxidase-peroxidase method of determination of ethanol content in fermented musts and wine products is described and compared to conventional methods routinely used in winemaking. The sensitivity, accuracy, and reliability of this method were determined. The results of ethanol determination in fermented musts and wines correlated well with the data obtained by refractometry (correlation coefficient R = 0.9595, p < 0.0001) and densitometry (correlation coefficient R = 0.9384, p < 0.0001). This method is less time- and labor-consuming and allows simultaneous testing a series of wine samples.
Subject(s)
Alcohol Oxidoreductases/chemistry , Ethanol/analysis , Peroxidase/chemistry , Wine/analysis , Densitometry , Pichia/enzymology , RefractometryABSTRACT
Enzymatic activity was studied in blood plasma, erythrocytes and liver tissue homogenate of rats treated with vegetable aromatic substances. 60 rats were kept within 3 months in atmosphere containing naturally occurring concentrations of monarde ester oil. Control animals were maintained in usual atmosphere of vivarium. In liver tissues of control animals glycolytic processes predominated as shown by the data on increased activities of lactate- and glucose-6-phosphate dehydrogenases. At the same time, activity of soluble microsomal enzyme acetyl esterase was decreased in liver tissue, while activity of these esterases was increased in blood plasma, thus suggesting that membrane structures were destabilized. Aromatic fractions of monarde ester oil, introduced into atmosphere, contributed to normalization of the enzymatic reactions studied.
