ABSTRACT
Background: Chemotherapy is typically the first-line treatment for the advanced stage of cancers. However, there are shortcomings with respect to conventional chemotherapy that limit therapeutic efficiency, including lack of tumor selectivity, systemic toxicity and drug resistance. Objective: A multifunctional nanoplatform was build using of hydrogel co-loaded containing cisplatin and Iron oxide-gold core-shell nanoparticles. The Au shell comprises the light response and the iron core can be utilized as a negative contrast agent in nanocomplex. Material and Methods: In this experimental study, KB cells derived from the epithelial cells located in the nasopharynx were exposed to different levels of concentration of hydrogel co-loaded with cisplatin and Iron oxide-gold core-shell nanoparticles. Afterwards, the cytotoxicity was determined using MTT assay. Results: The cytotoxicity results showed that this nanoplatforms has potent to create higher cytotoxicity in KB cells than free cisplatin, so that Fe-Au@Alg and Fe-Au@Alg with cisplatin mixed with laser irradiation exhibited a significant reduction in cell viability after 5 min. Conclusion: Hydrogel co-loaded with cisplatin and Iron oxide-gold core-shell nanoparticles are stable construct to combine chemo-photothermal therapy. Therefore, they can be used as a computed tomography-traceable nanocarrie, enabling us to monitor the delivery of therapeutics.
ABSTRACT
BACKGROUND: Gastric cancer (GC) is the third leading cause of cancer death, and most patients represent metastatic phenotype at the time of diagnosis. Urokinase plasminogen activator (uPA) system is well known for its critical roles in cancer cells invasion since uPA/uPA receptor (uPAR) overexpresses in several cancers. Subsequently, suppression of uPA/uPAR gene expression improves patients overall survival and prevents cancer progression. OBJECTIVES: The aim of the current study was to investigate possible effects of live Lactobacillus reuteri as a probiotic in inhibition of GC cells proliferation and invasion. MATERIALS AND METHODS: Human gastric adenocarcinoma epithelial cell line (AGS) cells were treated with different ratios of live L. reuteri and were incubated for 24, 48, and 72 h. Viability of cancer cells was measured with 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay, and the effects of L. reuteri on uPA/uPAR gene expression were assessed by real-time polymerase chain reaction. RESULTS: Our results showed that L. reuteri inhibits cell proliferation significantly in dose-dependent manner. Expressions of uPA and uPAR were downregulated followed by co-incubation of AGS cells and live L. reuteri compared to untreated-based line level. CONCLUSION: This study provides strong support in the role of L. reuteri in suppression of GC cell invasion by downregulation of pathways which is involved in extracellular matrix degradation such as uPA and uPAR.
