ABSTRACT
We tested the hypothesis whether microRNA-21 (miR-21) can detect CSA-AKI earlier than serum creatinine (sCr). A total of 103 patients scheduled to undergo cardiac surgery. CSA-AKI was defined as sCr > 0.3 mg/dl 24 h after surgery. The patients were divided into two groups according to whether or not developing AKI after surgery. Serum and urinary miR-21 were measured prior to, and 6, 12 and 24 h after surgery. Baseline serum and urinary levels of miR-21 in AKI group were lower than in non-AKI group. Moreover, the levels of miR-21 were significantly lower 6 h after surgery for serum, and 6 and 12 h after surgery for urine samples than those before surgery in AKI group. Area under the curve (AUC) of the receiver operating characteristic (ROC) values were 0.81 (95% CI: 0.65-0.97) for serum miR-21 (6 h after surgery), 0.90 (95% CI: 0.79-0.99) for urine (6 h after surgery), and 0.86 (95% CI: 0.71-0.98) for urine (12 h after surgery). While both postoperative serum and urinary miR-21 levels can predict AKI development, urinary miR-21 especially 6 h after surgery is a more reliable marker than serum miR-21 for detection of established CSA-AKI (Tab. 1, Fig. 3, Ref. 43).
Subject(s)
Acute Kidney Injury/blood , Cardiac Surgical Procedures , Creatinine/blood , MicroRNAs/blood , Postoperative Complications/blood , Acute Kidney Injury/diagnosis , Acute Kidney Injury/urine , Aged , Area Under Curve , Biomarkers/blood , Case-Control Studies , Early Diagnosis , Female , Humans , Kidney Function Tests , Male , MicroRNAs/urine , Middle Aged , Postoperative Complications/diagnosis , ROC CurveABSTRACT
BACKGROUND: Oxidative stress forms the foundation for the induction of multiple cellular pathways which can lead to the complications of diabetes mellitus that the most debilitating ones are diseases of the nervous system. In this study, we evaluated whether treadmill running could alleviate oxidative stress and apoptosis rate in the hippocampus of streptozotocin- induced diabetic rats. METHODS: Forty male Wistar rats were randomly divided into four groups (n=10): Control group (CR), exercised group (CE), diabetic group (DR) and diabetic-exercised group (DE). Diabetes was induced by injection of streptozotocin in male rats. All rats in the trained group run on a rodent motor-driven treadmill for eight weeks. At the end of eight weeks, hippocampi of animals were immediately removed on ice and kept frozen. The light supernatant was taken and stored at -80°C. They were used for determination of antioxidant enzymes and TBARs level. Index of apoptosis was detected by cell death detection ELISA Kit. RESULTS: Levels of TBARs in DR and DE groups were significantly higher than CR group. SOD and GPx activities significantly increased in CE group and decreased in DR group. CAT activity significantly decreased in DR group versus CR group. The apoptosis rate significantly increased and decreased in DR and CE groups respectively compared to CR. CONCLUSION: Exercise had beneficial effects in the diabetic exercised rats, possibly in part because of alterations in the ability to adapt to exercise-induced oxidative stress.
