ABSTRACT
In this paper we present the preliminary results of a novel biological analysis platform for early colon cancer detection using magnetic separation of magnetized markers. The platform consists of a microfluidic structure integrated with biosensors. Super-Paramagnetic Iron Oxide nanoparticles (SPIO-NPs) were functionalized with purified DNA Aptamer and their synthesis is described. In this paper, we also present the physicochemical results of the synthesized SPIO/Au-NPs characterized by TEM and XRD. Toxicity of our synthesized biomarkers on HCT116 cell line is discussed. Based on our findings, a concentration of 1mg/ml of our biomarkers added to 5 × 105 cells per well has no effect the viability of the human cells even after 24 hours.
Subject(s)
Biomarkers/analysis , Ferrosoferric Oxide/chemistry , Magnetite Nanoparticles/chemistry , Microfluidic Analytical Techniques/methods , Aptamers, Nucleotide/chemistry , Colonic Neoplasms/metabolism , Colonic Neoplasms/pathology , HCT116 Cells , Humans , Microfluidic Analytical Techniques/instrumentation , Microscopy, Electron, Transmission , Particle SizeABSTRACT
We describe in this paper cells sensing and manipulation methods, as well as platforms based on Lab-on-chip devices. Among other contributions, new circuit and microfluidic techniques, and packaging methods are proposed for efficient cells manipulation and detection. The proposed devices include high-sensitivity sensing circuits (200 mV/fF), low-pressure liquid injection interfaces (< 0.65 psi), low-voltage manipulation signals, direct-write microfluidic fabrication technique on top of CMOS based capacitive sensors. In addition, several types of electrode arrays (square and L-shaped) are used for the manipulation of various types of cells and particles.
Subject(s)
Biosensing Techniques/methods , Cells/metabolism , Diagnostic Techniques and Procedures , Metals/chemistry , Microfluidic Analytical Techniques/methods , Oxides/chemistry , Semiconductors , Dimethylpolysiloxanes/chemistry , Electric Capacitance , Electrodes , Electronics, Medical , Electrophoresis , PressureABSTRACT
We describe the emerging applications of direct-write CMOS-based lab-on-chip which consists of capacitive sensors integrated with microfluidic structures. The microfluidic components are implemented through direct-write microfabrication process (DWFP) on a variety of substrates including integrated circuits. We put forward the recent advances of DWFP for different applications while our focus is placed on biological testing through a novel on-chip capacitive measurement method. We thereafter reveal the viability of this approach for biosensing purposes by demonstrating and discussing the experimental results on micro-organisms. These results are in full agreement with the bio-interface model and other features presented throughout the paper.
ABSTRACT
A hybrid microfluidic/IC capacitive sensor is presented in this paper for highly integrated lab-on-chips (LoCs). We put forward the design and implementation of a charge based capacitive sensor array in 0.18-mum CMOS process. This sensor chip is incorporated with a microfluidic channel using direct-write microfluidic fabrication process (DWFP). The design, construction and experimental results as well are demonstrated using four different chemical solutions with known dielectric constants. The proposed highly sensitive CMOS capacitive sensor (ap530 mV/fF) along with low complexity DWFP emerges as clear favorite for LoC applications.
