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Eur Arch Paediatr Dent ; 25(3): 443-450, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38724869

ABSTRACT

PURPOSE: This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide-eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs). METHODS: The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay. RESULTS: The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001). CONCLUSION: Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE.


Subject(s)
Lactobacillus acidophilus , Metal Nanoparticles , Silver , Streptococcus mutans , Silver/pharmacology , Humans , Metal Nanoparticles/toxicity , Streptococcus mutans/drug effects , Lactobacillus acidophilus/drug effects , Zinc Oxide-Eugenol Cement/pharmacology , Zinc Oxide-Eugenol Cement/toxicity , In Vitro Techniques , Anti-Infective Agents/pharmacology , Dental Pulp/drug effects , Dental Pulp/cytology , Microbial Sensitivity Tests , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/toxicity , Cell Survival/drug effects , Stem Cells/drug effects , Materials Testing
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