Human parvovirus B19 in patients with beta thalassemia major from Tehran, Iran

BACKGROUND: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as beta-thalassemia major leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. We investigated the prevalence of parvovirus B19 among patients with beta thalassemia major attending the Zafar Adult Thalassemia Clinic in Tehran, Iran. METHODS: This cross-sectional study was performed to determine the presence of parvovirus B19 DNA in blood samples and parvovirus B19 genotypes in plasma samples of patients with thalassemia major. The population consisted of 150 patients with beta-thalassemia major who attended the Zafar clinic in Tehran. Specimens were studied using a real-time polymerase chain reaction assay. RESULTS: The prevalence of parvovirus B19 in our study population was 4%. Of 150 patients with thalassemia, six (4%) were positive for B19 DNA. There was no significant correlation between blood transfusion frequency and B19 DNA positivity. Finally, phylogenetic analysis of human parvovirus B19 revealed genotype I in these six patients. CONCLUSION: In this study, acute B19 infections were detected in patients with beta thalassemia major. Screening of such high-risk groups can considerably reduce the incidence and prevalence of B19 infection; thus, screening is required for epidemiologic surveillance and disease-prevention measures.

The effect of 24 h resting of old PCs at 22-24°Ð¡ in permeable bags on in vitro platelet parameters.

BACKGROUND: During transporting periods, platelet (PLT) concentrates (PCs) usually undergo a long period without agitation. Whether this interruption, improves quality and viability, or has deleterious effect at 72 h stored PCs, is here investigated. The aim of our study is to investigate effect of metabolic resting (24 h interruption of agitation) versus continue agitation of 72 h-stored PCs (old), prior to transfusion in the blood bank of Tehran. STUDY DESIGN AND METHODS: PCs were prepared using the platelet-rich plasma (PRP) method and stored in the permeable bags in the shaker/incubator, for 72 h at 20-24°C. Then by simply stopping the agitator, PCs remained at stationary condition without agitation for 24 h (WA24h) before sampling. In vitro measurements of PLT quality were carried out just after the termination of interruption period, and the results were compared with continuously agitated platelet within the same day (designated as control group, CA). In vitro variables which are measured were pH, Platelet count, swirling, Ristocetin-induced aggregation (GPIb-related function), LDH, PF4 release and P-selectin expression (activation marker). RESULTS: Compared with control group, the mean platelets PF4 release and P-selectin expression showed no significant differences (p=0.101, p=0.739 respectively). The mean level of pH was not significant (p=0.156); WA24h (7.12±0.14) and CA (7.17±0.11). Also Ristocetin-induced aggregation study showed significant differences (p=0.0281) between CA (76.6±3.2) and WA24h (62.69±21.43). Other in vitro variables between CA and WA groups including swirling, Platelet count and LDH showed no significant differences after 72 h of storage. CONCLUSIONS: We observed that 24 h resting of old PCs (WA24h) at 22-24°Ð¡ in permeable bags preserved pH, swirling, LDH, Ristocetin-induced aggregation and platelet's activation as good as control group after 72 h of storage.

Frequency & specificity of RBC alloantibodies in patients due for surgery in Iran.

BACKGROUND & OBJECTIVES: Red blood cell alloimmunization is common in patients receiving multiple blood transfusions. Since the probability of repeat transfusion increases with longer life expectancy, it is important to study to which extent alloimmunized patients with a history of transfusion are prone to form alloantibodies after transfusion events. The aim of this study was to retrospectively analyze the alloimmunization against RBCs among transfused patients who were to undergo elective surgery in Tehran, Iran. METHODS: A total of 3092 occasionally transfused patients, who were to undergo elective surgery, in four hospitals in Tehran were included in the study. For patients with alloantibodies, the data about sex, date of birth, history of transfusion, surgery, abortion and alloantibody specificity were collected. RESULTS: Clinically significant alloantibodies were found in 30 patients. The presence of positive antibodies in the patients for whom cross-match had been done was one per cent. Most of them had surgery history or transfusion record during the preceding year. The three most frequent alloantibodies were anti-K (23.53%), anti- E (20.59%) and anti-c (17.56%). INTERPRETATION & CONCLUSIONS: The most common clinically significant alloantibodies identified in men and women were anti-K and anti-E, respectively. The most common causes of alloimmunization for men were surgery history and transfusion record and for women pregnancy.