[Evidence of intraepithelial lymphocytes through immunocytochemistry stains in children with celiac disease]. / Evidentierea limfocitelor intraepiteliale prin coloratii imunohistochimice la copiii cu boala celiaca.

UNLABELLED: The purpose of of this study is to identify IELs through immunocytochemistry stains for counting infiltration duodenal mucosa with T lymphocytes in children with celiac disease. MATERIAL AND METHOD: It was selected up simptomatology (abdominal pain, anorexia, failure thrive, chronic diarheea), features immunology(positifs tissue transglutaminase antibodies) and considering the histopathologic results (IELs > 30/100 enterocytes, crypt hyperplasia and villous atrophy) we made two groups: first group (13 children with celiac disease of 53 children with celiac disease) and second group (13 children with chronic duodenitis nonspecific). For evidence of intraepithelial lymphocytes T using immunocytochemistry tehnicque (immunohistochemical staining) Envision with antibody anti CD 3 from Dako firme. Corresponding Marsh clasification considering patological IELs > 30/100 enterocytes. Statitics method was used The Student' t test. RESULTS: Analyse IELs in two groups our show difference statistics to demonstrate with the Student' t test (t = -12.237; p = 0.003). CONCLUSION: An increase IELs is helpful in recognising early and bordeline, but the finding is not specific. The histopathologic exam remains the gold standard for celiac disease.

[Helicobacter pylori infection in children with celiac disease]. / Infectia cu Helicobacter pylori la copiii cu boala celiaca.

UNLABELLED: The purpose of this study is to evaluate symptomatology, endoscopic and histopathologic changes of Helicobacter pylori infection and gastritis lesions without Helicobacter pylori infection on children diagnosed with celiac disease. MATERIAL AND METHOD: 15 children under gluten-free diet were selected and, because of the recurrence of the dyspeptic syndrome, an upper digestive endoscopy associated with histopathologic exam was performed. Considering the histopathologic result we made two groups: first group (8 children with celiac disease and Helicobacter pylori infection) and second group (7 children with celiac disease without Helicobacter pylori infection, but associated with gastritis lesions). RESULTS: The main symptom was diffuse abdominal pain in both groups. The endoscopic antrum aspects were congestive with striped aspect (first group--12.5%, second group--42.9%) and congestive with nodulation (first group--25%, second group--14.3%). The histopathologic diagnosis were: moderate active chronic pangastritis (first group--25%, second group--14.3%) moderate active chronic gastritis (first group--25%,second group--14.3%), lymphocytic gastritis (first group--12.5%, second group--14.3%). CONCLUSION: The histopathologic exam remains the gold standard for celiac disease, gastritis lesions and Helicobacter pylori infection.