ABSTRACT
The photodynamic treatment is a non-aggressive and clinically accepted procedure for removing selected cancer cells with the activation of a photosensitizer agent at a specific light. In this study, the zinc porphyrin (Zn[TPP]) was prepared and encapsulated into the MIL-101 (Zn[TPP]@MIL-101). It was used in photodynamic therapy (PDT) against MCF-7 breast cancer cells under a red light-emitting diode. The structure, morphology, surface area, and compositional changes were investigated using conventional characterization methods including FTIR, FESEM, EDX, and BET analyses. The MTT assay was performed under light and dark conditions to explore the ability of Zn[TPP]@MIL-101 in PDT. The results have demonstrated the IC50 of 14.3 and 81.6 mg/mL for light and dark groups, respectively. As the IC50 revealed, the Zn[TPP]@MIL-101 could efficiently eradicate cancer cells using PDT.
Subject(s)
Breast Neoplasms , Photochemotherapy , Porphyrins , Humans , Female , Porphyrins/therapeutic use , Photochemotherapy/methods , Breast Neoplasms/drug therapy , Photosensitizing Agents/therapeutic use , Zinc/pharmacology , Zinc/therapeutic use , Cell Line, TumorABSTRACT
In this study for the very first time, zinc tetraphenylporphyrin (ZnTPP) was loaded into MIL-101 (Zn[TPP]@MIL-101) to perform an adsorptive and photocatalytic dye removal. The physicochemical attributes of the catalyst were thoroughly determined by the usage of XRD, FTIR, FESEM, BET, UV-vis, and inductively coupled plasma (ICP). The obtained XRD pattern exhibited the phase purity of MIL-101 and its structural stability. The solid-phase diameter of the catalyst was observed to be ~ 270.76 ± 119.95 nm, while its gas adsorption data was indicative of a decrease in the specific surface area after the loading of ZnTPP. The ICP analysis displayed the amount of encapsulated Zn[TPP] (~ 17%) in MIL-101. The UV-vis confirmed the presence of Zn[TPP] in MIL-101 with the lack of any interferences or overlaps with the λmax of methylene blue (MB) with the support. The dye removal of MB was investigated under dark conditions (adsorption) and UV light (photodegradation). The observed adsorption under dark conditions using Zn[TPP]@MIL-101 (99.27% yield) demonstrated a superior dye removal in comparison to the cases of photodegradation of MB by MIL-101 and Zn[TPP]@MIL-101 or adsorption by MIL-101. In conformity to the gathered results, [ZnTPP] was able to increase the adsorption capacity at pH = 7 at room temperature.
