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1.
J Gerontol A Biol Sci Med Sci ; 74(11): 1709-1715, 2019 10 04.
Article in English | MEDLINE | ID: mdl-30590424

ABSTRACT

Visceral adipose tissue (VAT) inflammation plays a central role in longevity and multiple age-related disorders. Cellular senescence (SEN) is a fundamental aging mechanism that contributes to age-related chronic inflammation and organ dysfunction, including VAT. Recent studies using heterochronic parabiosis models strongly suggested that circulating factors in young plasma alter the aging phenotypes of old animals. Our study investigated if young plasma rescued SEN phenotypes in the VAT of aging mice. With heterochronic parabiosis model using young (3 months) and old (18 months) mice, we found significant reduction in the levels of pro-inflammatory cytokines and altered adipokine profile that are protective of SEN in the VAT of old mice. These data are indicative of protection from SEN of aging VAT by young blood circulation. Old parabionts also exhibited diminished expression of cyclin-dependent kinase inhibitors (CDKi) genes p16 (Cdkn2a) and p21 (Cdkn1a/Cip1) in the VAT. In addition, when exposed to young serum condition in an ex vivo culture system, aging adipose tissue-derived stromovascular fraction cells produced significantly lower amounts of pro-inflammatory cytokines (MCP-1 and IL-6) compared to old condition. Expressions of p16 and p21 genes were also diminished in the old stromovascular fraction cells under young serum condition. Finally, in 3T3-preadipocytes culture system, we found reduced pro-inflammatory cytokines (Mcp-1 and Il-6) and diminished expression of cyclin-dependent kinase inhibitor genes in the presence of young serum compared to old serum. In summary, this study demonstrates that young milieu is capable of protecting aging adipose tissue from SEN and thereby inflammation.


Subject(s)
Aging/genetics , Chemokine CCL2/genetics , Cyclin-Dependent Kinase Inhibitor Proteins/genetics , Cytokines/metabolism , Parabiosis/methods , Wounds and Injuries/metabolism , Age Factors , Aging/physiology , Analysis of Variance , Animals , Cells, Cultured , Cellular Senescence/genetics , Cellular Senescence/physiology , Disease Models, Animal , Inflammation/genetics , Inflammation/metabolism , Interleukin-6/metabolism , Intra-Abdominal Fat/metabolism , Male , Mice, Inbred C57BL , Random Allocation , Real-Time Polymerase Chain Reaction/methods , Sensitivity and Specificity , Wound Healing/genetics
2.
Aging (Albany NY) ; 10(4): 764-774, 2018 04 25.
Article in English | MEDLINE | ID: mdl-29695642

ABSTRACT

Adipose tissue dysfunction is associated with inflammation, metabolic syndrome and other diseases in aging. Recent work has demonstrated that compromised autophagy activity in aging adipose tissue promotes ER stress responses, contributing to adipose tissue and systemic inflammation in aging. Phosphatidylinositol 3-kinase catalytic subunit type 3 (Pik3c3) is an 887 amino acid lipid kinase that regulates intracellular membrane trafficking and autophagy activity. To address the mechanistic link between autophagy and ER stress response in aging adipose tissue, we generated a line of adipose tissue-specific Pik3c3 knock out (~mutant mice) with the Fabp4 (Fatty acid binding protein 4) promoter driven Cre recombinase system. We found elevated ER stress response signaling with reduced autophagy activity without any significant change on adiposity or glucose tolerance in early life of Pik3c3 mutant mice. Interestingly, middle- and old-aged mutant mice exhibited improved glucose tolerance (GTT) and reduced adiposity compared to age and sex-matched littermates. In addition, adipose tissue-specific Pik3c3 mutants display reduced expression of adiposity-associated genes with the signature of adipose tissue browning phenotypes in old age. Overall, the results suggest that altered adipose tissue characteristics due to autophagy inhibition early in life has beneficial effects that promote adipose tissue browning and improves glucose tolerance in late-life.


Subject(s)
Adipose Tissue, White/pathology , Aging/pathology , Autophagy/physiology , Class III Phosphatidylinositol 3-Kinases/genetics , Adipose Tissue, Brown/metabolism , Adipose Tissue, Brown/pathology , Adipose Tissue, White/metabolism , Adiposity/physiology , Animals , Endoplasmic Reticulum Stress/genetics , Inflammation/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout
3.
Aging (Albany NY) ; 8(10): 2525-2537, 2016 10 24.
Article in English | MEDLINE | ID: mdl-27777379

ABSTRACT

Adipose tissue dysfunction in aging is associated with inflammation, metabolic syndrome and other diseases. We propose that impaired protein homeostasis due to compromised lysosomal degradation (micro-autophagy) might promote aberrant ER stress response and inflammation in aging adipose tissue. Using C57BL/6 mouse model, we demonstrate that adipose tissue-derived stromal vascular fraction (SVF) cells from old (18-20 months) mice have reduced expression of autophagy markers as compared to the younger (4-6 months) cohort. Elevated expressions of ER-stress marker CHOP and autophagy substrate SQSTM1/p62 are observed in old SVFs compared to young, when treated with either vehicle or with thapsigargin (Tg), an ER stress inducer. Treatment with bafilomycin A1 (Baf), a vacuolar-type H (+)-ATPase, or Tg elevated expressions of CHOP, and SQSTM1/p62 and LC-3-II, in 3T3-L1-preadipocytes. We also demonstrate impaired autophagy activity in old SVFs by analyzing increased accumulation of autophagy substrates LC3-II and p62. Compromised autophagy activity in old SVFs is correlated with enhanced release of pro-inflammatory cytokines IL-6 and MCP-1. Finally, SVFs from calorie restricted old mice (CR-O) have shown enhanced autophagy activity compared to ad libitum fed old mice (AL-O). Our results support the notion that diminished autophagy activity with aging contributes to increased adipose tissue ER stress and inflammation.


Subject(s)
Adipose Tissue/metabolism , Aging/metabolism , Autophagy/physiology , Endoplasmic Reticulum Stress/physiology , Inflammation/metabolism , Adipocytes/drug effects , Adipocytes/metabolism , Adipose Tissue/drug effects , Animals , Autophagy/drug effects , Chemokine CCL2/metabolism , Endoplasmic Reticulum Stress/drug effects , Enzyme Inhibitors/pharmacology , Interleukin-6/metabolism , Lysosomes/metabolism , Mice , Mice, Inbred C57BL , Thapsigargin/pharmacology
4.
J Gerontol A Biol Sci Med Sci ; 70(11): 1320-9, 2015 Nov.
Article in English | MEDLINE | ID: mdl-25324219

ABSTRACT

Adipose tissue inflammation has been linked to age-related metabolic diseases. However, the underlying mechanisms are poorly understood. Adipose tissue inflammation and insulin resistance in diet associated obesity has been correlated with aberrant endoplasmic reticulum (ER) stress. This study was undertaken to test our hypothesis that increased ER stress response contributes to age-associated adipose tissue inflammation. We found elevated ER stress response in adipose tissue of old (18-20 months) compared to young (4-6 months) mice. Elevated ER stress markers BIP (GRP78), CHOP, cleaved-ATF-6, phospho-IRE1α, and XBP-1 were observed in old compared to young adipose tissue stromal cells. Additionally, old adipose tissue stromal cells were more sensitive to an ER stress inducer, thapsigargin. Similar experiments with adipose tissue macrophages showed elevated Chop and Bip expression in old adipose tissue macrophages when induced with thapsigargin. Treatment of chemical chaperone 4-phenyle-butyric acid alleviated ER stress in adipose tissue stromal cells and adipose tissue macrophages and attenuated the production of IL-6 and MCP-1 by adipose tissue stromal cells, and TNF-α by adipose tissue macrophages from both young and old mice. Finally, old mice fed with 4-phenyle-butyric acid have reduced expression of ER stress and inflammatory cytokine genes. Our data suggests that an exaggerated ER stress response in aging adipose tissue contributes to age-associated inflammation that can be mitigated by treatment with chemical chaperones.


Subject(s)
Adipose Tissue/metabolism , Adipose Tissue/pathology , Endoplasmic Reticulum Stress/physiology , Macrophages/physiology , Stromal Cells/physiology , Activating Transcription Factor 6/genetics , Activating Transcription Factor 6/metabolism , Age Factors , Animals , Cell Culture Techniques , Cytokines/genetics , Cytokines/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Endoplasmic Reticulum Chaperone BiP , Endoribonucleases/genetics , Endoribonucleases/metabolism , Enzyme Inhibitors , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Inflammation , Male , Mice , Phenylbutyrates , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , RNA, Messenger/metabolism , Regulatory Factor X Transcription Factors , Thapsigargin , Transcription Factor CHOP/genetics , Transcription Factor CHOP/metabolism , Transcription Factors/genetics , Transcription Factors/metabolism , X-Box Binding Protein 1
5.
Invest Ophthalmol Vis Sci ; 54(7): 4503-11, 2013 Jul 02.
Article in English | MEDLINE | ID: mdl-23745007

ABSTRACT

PURPOSE: Mutations in the RP2 gene are associated with 10% to 15% of X-linked retinitis pigmentosa (XLRP), a debilitating disorder characterized by the degeneration of retinal rod and cone photoreceptors. The molecular mechanism of pathogenesis of photoreceptor degeneration in XLRP-RP2 has not been elucidated, and no treatment is currently available. This study was undertaken to investigate the pathogenesis of RP2-associated retinal degeneration. METHODS: We introduced loxP sites that flank exon 2, a mutational hotspot in XLRP-RP2, in the mouse Rp2 gene. We then produced Rp2-null allele using transgenic mice that expressed Cre-recombinase under control of the ubiquitous CAG promoter. Electroretinography (ERG), histology, light microscopy, transmission electron microscopy, and immunofluorescence microscopy were performed to ascertain the effect of ablation of Rp2 on photoreceptor development, function, and protein trafficking. RESULTS: Although no gross abnormalities were detected in the Rp2(null) mice, photopic (cone) and scotopic (rod) function as measured by ERG showed a gradual decline starting as early as 1 month of age. We also detected slow progressive degeneration of the photoreceptor membrane discs in the mutant retina. These defects were associated with mislocalization of cone opsins to the nuclear and synaptic layers and reduced rhodopsin content in the outer segment of mutant retina prior to the onset of photoreceptor degeneration. CONCLUSIONS: Our studies suggest that RP2 contributes to the maintenance of photoreceptor function and that cone opsin mislocalization represents an early step in XLRP caused by RP2 mutations. The Rp2(null) mice should serve as a useful preclinical model for testing gene- and cell-based therapies.


Subject(s)
Genes, X-Linked/genetics , Genetic Diseases, X-Linked/genetics , Intracellular Signaling Peptides and Proteins/deficiency , Membrane Proteins/deficiency , Opsins/metabolism , Photoreceptor Cells/pathology , Retinitis Pigmentosa/genetics , Animals , Disease Models, Animal , Electroretinography , Eye Proteins/genetics , GTP-Binding Proteins , Genetic Diseases, X-Linked/pathology , Genetic Diseases, X-Linked/physiopathology , Intracellular Signaling Peptides and Proteins/genetics , Membrane Proteins/genetics , Mice , Retinitis Pigmentosa/pathology , Retinitis Pigmentosa/physiopathology
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