Removal of hazardous textile dye from simulated wastewater by municipal organic solid waste charcoal using machine learning approaches: Kinetics, isotherm, and thermodynamics.

This study focuses on the probable use of municipal organic solid waste charcoal (MOSWC) as an adsorbent for Methyl orange (MO) adsorption. The prepared MOSWC is characterized by FE-SEM and FT-IR. Batch adsorption experiments were conducted with the influencing of different operational conditions namely time of contact (1-180 min), adsorbate concentration (60-140 mg/L), adsorbent dose (1-5 g/L), pH (3-11), and temperature (25-60 °C). The high coefficient value (R2 = 0.96) of the process optimization model suggests that this model was significant, where pH and adsorbent dose expressively stimulus adsorption efficiency including 40.11 mg/g at pH (3), MO concentration (100 mg/L), and MOSWC dose (1 g/L). Furthermore, the machine learning approaches (ANN and BB-RSM) revealed a good association between the tested and projected values. The highest monolayer adsorption capacity of MO was 90.909 mg/g. Pseudo-second-order was the well-suited kinetics, where Langmuir isotherm could explain better for equilibrium adsorption data. Thermodynamic study shows MO adsorption is favourable, exothermic, and spontaneous. Finally, this study indicates that MOSWC could be a potential candidate for the adsorption of MO from wastewater.

Microalgae and biogas: a boon to energy sector.

The global energy generation market immensely depends on fossil fuels which balances our survival on this planet. Energy can be called as the "master element" for our daily needs, starting from household power supply, agricultural purpose, automobile and transportation, industrial workload to economic and research domains. Fuel switching initiatives are being adapted by environmentalist and scientists to bring a novel sustainable source of energy. An environment and renewable alternative to fossil fuels are a must. Over the years, the world has shifted toward generating green fuels immensely. One such potential alternative to fossil fuels are biogases. Being versatile and renewable in nature, it has drawn immense attention globally. Despite having such potentials there exist some major drawbacks which mainly deal with the starting material. One such source for biogases can be microalgae. Microalgae based biogas production can produce huge amount of energy and that has been implemented by many foreign countries and their companies. Despite being in use in many countries, there are issues which needs to be addressed which will overall improve the biogas potential from microalgae even more. This review mainly focuses on generation of biogas from microalgae as a feedstock which are very economical and sustainable in its nature, presenting improvement strategies which can be impended to boost the over biogas sector globally.

A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement.

Recent research reports have shown that plant pests and pathogens have depleted the crop yield widely, which has led to an increased dependence on commercial pesticides and fungicides. Increased usage of these pesticides has also shown adverse effects on the environment, therefore many techniques have been implemented for solving the issue, some of which include using nanobioconjugates, RNA(i), which put into use double-stranded RNAs to inhibit gene expression. A more innovative and eco-friendly strategy includes spray induced gene silencing, which is being increasingly implemented. This review delves into the eco-friendly approach of spray induced gene silencing (SIGS) in combination with nanobioconjugates, which have been used concerning various plant hosts and their pathogens to provide improved protection. Furthermore, nanotechnological advancements have been understood by addressing the scientific gaps to provide a rationale for the development of updated techniques in crop protection.

Rat carboxylesterase ES-4 enzyme functions as a major hepatic neutral cholesteryl ester hydrolase.

Although esterification of free cholesterol to cholesteryl ester in the liver is known to be catalyzed by the enzyme acyl-coenzyme A:cholesterol acyltransferase, ACAT, the neutral cholesteryl ester hydrolase (nCEH) that catalyzes the reverse reaction has remained elusive. Because cholesterol undergoes continuous cycling between free and esterified forms, the steady-state concentrations in the liver of the two species and their metabolic availability for pathways, such as lipoprotein assembly and bile acid synthesis, depend upon nCEH activity. On the basis of the general characteristics of the family of rat carboxylesterases, we hypothesized that one member, ES-4, was a promising candidate as a hepatic nCEH. Using under- and overexpression approaches, we provide multiple lines of evidence that establish ES-4 as a bona fide endogenous nCEH that can account for the majority of cholesteryl ester hydrolysis in transformed rat hepatic cells and primary rat hepatocytes.

IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta.

We describe a mouse strain in which B cell development relies either on the expression of membrane-bound immunoglobulin (Ig) gamma1 or mu heavy chains. Progenitor cells expressing gamma1 chains from the beginning generate a peripheral B cell compartment of normal size with all subsets, but a partial block is seen at the pro- to pre-B cell transition. Accordingly, gamma1-driven B cell development is disfavored in competition with developing B cells expressing a wild-type (WT) IgH locus. However, the mutant B cells display a long half-life and accumulate in the mature B cell compartment, and even though partial truncation of the Ig alpha cytoplasmic tail compromises their development, it does not affect their maintenance, as it does in WT cells. IgG1-expressing B cells showed an enhanced Ca(2+) response upon B cell receptor cross-linking, which was not due to a lack of inhibition by CD22. The enhanced Ca(2+) response was also observed in mature B cells that had been switched from IgM to IgG1 expression in vivo. Collectively, these results suggest that the gamma1 chain can exert a unique signaling function that can partially replace that of the Ig alpha/beta heterodimer in B cell maintenance and may contribute to memory B cell physiology.

Regulation of B cell development and B cell signalling by CD22 and its ligands alpha2,6-linked sialic acids.

CD22 is an inhibitory co-receptor of B cell receptor (BCR)-mediated signalling which binds specifically to glycan ligands containing alpha2,6-linked sialic acids. This interaction modulates the CD22 activity by an unknown mechanism. Mice deficient for ST6GalI, the enzyme that generates alpha2,6-linked sialic acids, show an immunodeficient and opposing phenotype to CD22-deficient mice. By generating mice double-deficient for this receptor/ligand pair, we analysed its influence on B cell maturation and signalling. Both ST6GalI-deficient and ST6GalI x CD22-deficient mice showed normal B cell development, but an impaired marginal zone B cell population in the spleen. Both types of mutant mice also showed a reduced population of bone marrow recirculating B cells, a defect previously detected in CD22-/- mice. In adoptive transfer experiments, a migration defect of wild-type B cells to the bone marrow of ST6GalI-deficient mice was found. This suggests a direct involvement of CD22 and its ligands 2,6Sia in a homing process of recirculating B cells to the bone marrow. Interestingly, defective B cell Ca2+ signalling and proliferation of ST6Gal-/- mice was rescued in ST6GalI x CD22-deficient mice. This points to a new mechanism of BCR signal regulation by CD22 and its ligand.

B cell defects in SLP65/BLNK-deficient mice can be partially corrected by the absence of CD22, an inhibitory coreceptor for BCR signaling.

CD22 is an inhibitory coreceptor for B cell receptor (BCR) signaling. The inhibition is most likely mediated by activation of SHP-1. We found that SLP65/BLNK reaches maximal tyrosine-phosphorylation at earlier time points in CD22(-/-) than in wild type B cells upon BCR cross-linking, suggesting that SLP65/BLNK is a substrate of SHP-1. However, in contrast to the defective Ca(2+) mobilization of SLP65/BLNK(-/-) B cells, there was a clear Ca(2+) response in SLP65/BLNKxCD22 double-deficient B cells. This implies that SLP65/BLNK is not the sole target of SHP-1 in the regulation of the Ca(2+) signaling strength. While SLP65(-/-) mice show several blocks of B cell differentiation, in SLP65/BLNK x CD22 double-deficient mice the maturation block of B cells in the spleen was partially rescued. However, the proliferative responses of B cells from both SLP65/BLNK(-/-) and double-deficient mice were defective after IgM- or CD40-stimulation. These results show that SLP65/BLNK is not absolutely essential for Ca(2+) induction in B cells, because the deficiency of this adapter can be by-passed by the additional deletion of an inhibitory receptor. Furthermore, these experiments suggest that B cell maturation in the spleen is directly dependent on the strength of BCR-derived Ca(2+) signals.