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1.
Kulak Burun Bogaz Ihtis Derg ; 20(1): 44-7, 2010.
Article in English | MEDLINE | ID: mdl-20163337

ABSTRACT

A 56-year-old Caucasian man was referred to our clinic with the complaint of right sided unilateral nasal blockage which had been present for one year. Anterior rhinoscopy and computed tomograpy scan revealed a mass, filling the right nostril completely and lying in the right inferior meatus inseparable from the inferior turbinate. Following the biopsy, histopathological examination and immunohistochemical analyses, the diagnosis of Mantle cell lymphoma was established. To our knowledge, there have been no previous reports of a Mantle cell lymphoma presenting as an intranasal mass in the literature. In this article the clinical, radiological and pathological features as well as recent advances in treatment are discussed in the light of current literature.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Lymphoma, Mantle-Cell/pathology , Nose Neoplasms/pathology , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Biopsy , Combined Modality Therapy , Cyclophosphamide/administration & dosage , Doxorubicin/administration & dosage , Humans , Immunohistochemistry , Immunotherapy/methods , Lymphoma, Mantle-Cell/diagnostic imaging , Lymphoma, Mantle-Cell/drug therapy , Lymphoma, Mantle-Cell/immunology , Male , Middle Aged , Nasal Cavity/diagnostic imaging , Nasal Cavity/pathology , Nose Neoplasms/diagnostic imaging , Nose Neoplasms/drug therapy , Nose Neoplasms/immunology , Prednisone/administration & dosage , Tomography, X-Ray Computed , Treatment Outcome , Vincristine/administration & dosage
2.
Toxicol Appl Pharmacol ; 212(1): 45-58, 2006 Apr 01.
Article in English | MEDLINE | ID: mdl-16112157

ABSTRACT

Previous in vivo studies from several laboratories had shown remarkable curative effect of methylglyoxal on cancer-bearing animals. In contrast, most of the recent in vitro studies have assigned a toxic role for methylglyoxal. The present study was initiated with the objective to resolve whether methylglyoxal is truly toxic in vivo and to reassess its therapeutic potential. Four species of animals, both rodent and non-rodent, were treated with different doses of methylglyoxal through oral, subcutaneous and intravenous routes. Acute (treatment for only 1 day) toxicity tests had been done with mouse and rat. These animals received 2, 1 and 0.3 g of methylglyoxal/kg of body weight in a day through oral, subcutaneous and intravenous routes respectively. Chronic (treatment for around a month) toxicity test had been done with mouse, rat, rabbit and dog. Mouse, rat and dog received 1, 0.3 and 0.1 g of methylglyoxal/kg of body weight in a day through oral, subcutaneous and intravenous routes respectively. Rabbit received 0.55, 0.3 and 0.1 g of methylglyoxal/kg of body weight in a day through oral, subcutaneous and intravenous routes respectively. It had been observed that methylglyoxal had no deleterious effect on the physical and behavioral pattern of the treated animals. Fertility and teratogenecity studies were done with rats that were subjected to chronic toxicity tests. It had been observed that these animals produced healthy litters indicating no damage of the reproductive systems as well as no deleterious effect on the offspring. Studies on several biochemical and hematological parameters of methylglyoxal-treated rats and dogs and histological studies of several organs of methylglyoxal-treated mouse were performed. These studies indicated that methylglyoxal had no apparent deleterious effect on some vital organs of these animals. A detailed pharmacokinetic study was done with mouse after oral administration of methylglyoxal. The effect of methylglyoxal alone and in combination with creatine and ascorbic acid on cancer-bearing animals had been investigated by measuring the increase in life span and tumor cell growth inhibition. The results indicated that anticancer effect of methylglyoxal was significantly augmented by ascorbic acid and further augmented by ascorbic acid and creatine. Nearly 80% of the animals treated with methylglyoxal plus ascorbic acid plus creatine were completely cured and devoid of any malignant cells within the peritoneal cavity.


Subject(s)
Antineoplastic Agents/pharmacokinetics , Antineoplastic Agents/toxicity , Ascorbic Acid/therapeutic use , Carcinoma, Ehrlich Tumor/drug therapy , Creatine/therapeutic use , Pyruvaldehyde/pharmacokinetics , Pyruvaldehyde/toxicity , Vitamins/therapeutic use , Animals , Antineoplastic Agents/therapeutic use , Body Weight/drug effects , Carcinoma, Ehrlich Tumor/pathology , Dogs , Drug Synergism , Enzymes/blood , Female , Fertility/drug effects , Longevity/drug effects , Male , Mice , Neoplasm Transplantation , Pyruvaldehyde/therapeutic use , Rabbits , Rats , Reproduction/drug effects , Species Specificity , Survival Analysis , Teratogens/toxicity
3.
Indian J Biochem Biophys ; 41(1): 7-13, 2004 Feb.
Article in English | MEDLINE | ID: mdl-22896902

ABSTRACT

Ehrlich ascites carcinoma (EAC) cell glyceraldehyde-3-phosphate dehydrogenase (GA3PD) (EC. 1.2.1.12) was completely inactivated by diethyl pyrocarbonate (DEPC), a fairly specific reagent for histidine residues in the pH range of 6.0-7.5. The rate of inactivation was dependent on pH and followed pseudo-first order reaction kinetics. The difference spectrum of the inactivated and native enzymes showed an increase in the absorption maximum at 242 nm, indicating the modification of histidine residues. Statistical analysis of the residual enzyme activity and the extent of modification indicated modification of one essential histidine residue to be responsible for loss of the catalytic activity of EAC cell GA3PD. DEPC inactivation was protected by substrates, D-glyceraldehyde-3-phosphate and NAD, indicating the presence of essential histidine residue at the substrate-binding region of the active site. Double inhibition studies also provide evidence for the presence of histidine residue at the active site.


Subject(s)
Carcinoma, Ehrlich Tumor/metabolism , Gene Expression Regulation, Enzymologic , Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/biosynthesis , Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/chemistry , Histidine/chemistry , Animals , Catalytic Domain , Diethyl Pyrocarbonate/chemistry , Dithionitrobenzoic Acid/chemistry , Hydrogen-Ion Concentration , Hydroxylamine/chemistry , Kinetics , Mice
4.
Indian J Biochem Biophys ; 39(5): 332-41, 2002 Oct.
Article in English | MEDLINE | ID: mdl-22905379

ABSTRACT

3-Phosphoglycerate kinase (3-PGK) has been purified to apparent homogeneity from Ehrlich ascites carcinoma (EAC) cells by (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography. The enzyme has been partially characterized and compared with the characteristics of this enzyme of other normal and malignant cells. The EAC cell 3-PGK is composed of a single subunit of 47 kDa. It has a broad pH optimum (pH 6.0-7.5) for its enzymatic activity. The apparent Km values of 3-phosphoglycerate (3-PGA) and ATP for 3-PGK have been found out to be 0.25 mM and 0.1 mM respectively. Similar to 3-PGK of other cells, the EAC enzyme requires either Mg2+ or Mn2+ for full activity; the optimum concentrations of Mg2+ and Mn2+ are 0.8 mM and 0.5 mM respectively. When ATP and 3-PGA act as substrates, ADP, the reaction product of 3-PGK-catalyzed reaction has been found to inhibit this enzyme. Kinetic studies were made on the inhibition of ADP in presence of the substrates ATP and 3-PGA. Attempts to hybridize 3-PGK and glyceraldehyde-3-phosphate dehydrogenase of EAC cells by NAD or glutaraldehyde were unsuccessful.


Subject(s)
Carcinoma, Ehrlich Tumor/metabolism , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Neoplastic , Phosphoglycerate Kinase/chemistry , Phosphoglycerate Kinase/isolation & purification , Adenosine Diphosphate/chemistry , Adenosine Triphosphate/metabolism , Animals , Chromatography, Gel , Chromatography, Ion Exchange/methods , Edetic Acid/chemistry , Hydrogen-Ion Concentration , Kinetics , Magnesium/chemistry , Manganese/chemistry , Mercaptoethanol/pharmacology , Mice , Neoplasm Transplantation , Nucleic Acid Hybridization
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