Self Emulsifying Delivery System of Cissus quadrangularis: Evidence of Enhanced Efficacy and Promising Pharmacokinetic Profile in the Management of Osteoporosis.

Treatment therapies used to manage osteoporosis are associated with severe side effects. So worldwide herbs are widely studied to develop alternative safe & effective treatments. Cissus quadrangularis (CQ) has a significant role in bone health and fracture healing. It is documented that its extracts increase osteoblastic differentiation & mineralization. Currently, Cissus quadrangularis is available in the form of tablets in the market for oral delivery. But these conventional forms are associated with poor bioavailability. There is a need for a novel drug delivery system with improving oral bioavailability. Therefore, a Cissus quadrangularis-loaded self-emulsifying drug delivery system (CQ-SEDDS) was developed which disperses rapidly in the gastrointestinal fluids, yielding nano-emulsions containing a solubilized drug. This solubilized form of the drug can be easily absorbed through lymphatic pathways and bypass the hepatic first-pass effect. The emulsification efficiency, zeta potential, globule size, in-vitro dissolution, ex-vivo, in-vivo and bone marker studies were performed to assess the absorption and permeation potential of CQ incorporated in SEDDS. CQ-SEDDS with excipients Tween 80, Cremophor RH40, Transcutol HP & α-Tocopherol acetate had shown about 76% enhancement in the bioavailability of active constituents of CQ. This study provided the pre-clinical data of CQ-SEDDS using osteoporotic rat model studies.

Pearl millet flour and green gram milk based probiotic beverage.

The probiotic beverage was developed using germinated and ungerminated pearl millet flour and green gram milk. The germinated and ungerminated pearl millet flour was added to green gram milk at different concentrations (0.5-2.5 %) along with sugar and cardamom. The mixtures were then inoculated with probiotic bacteria Lactobacillus acidophilus incubated at 37 °C for 6 h. Characterization of probiotic beverages was carried out during storage at (4 ± 1)°C for 21 days. The germinated flour beverage had high acidity as compared to the ungerminated flour beverage. The probiotic count in germinated and ungerminated flour beverages ranged from 8.19 to 8.77 × 107 and 8.04 to 8.52 × 107 log CFU/mL, respectively. Antioxidant activity, polyphenol content increased with an increase in the concentration of flour in the beverage. The LC-MS analysis found the existence of vitexin and isovitexin as the main polyphenolic compounds in the probiotic beverage. Non-dairy probiotic beverage prepared with 0.5 % germinated millet flour gave the best taste, color, texture, and rheological properties.

Comprehensive review on pulsed electric field in food preservation: gaps in current studies for potential future research.

In pulsed electric field (PEF) method sources of high voltage pulses are placed amid two electrodes in to fluid or paste type foods. Electricity is passed between two electrodes to sterilize the food. Almost all PEF technology entails the use of this technology in milk and milk product processing, eggs, poultry, juices and other liquid foods to prevent microorganisms. PEF technology, one of the promising methods of non-thermal preservation of food, can address the biological hazards efficiently. Recently available research papers explored PEF technology not only to facilitate the inactivation of microorganisms but also to alleviate the pressing competence for juice extraction purpose from plants for food application and also to intensify the drying and dehydration process of food. Most of the literatures are available on killing of microorganisms using PEF technology but the reports on influence of PEF technology on quality parameters of food after treatment and about their acceptability are limited. Now the technology is becoming popular and many recent papers reported about better yield and excellent quality of nutrient extracted by using PEF technology.

Utilization and characterization of flaxseed oil in ultrasonically emulsified mango beverage.

The present study described the formation of stable emulsion of flaxseed oil (0%, 0.25%, 0.50%, 0.75% v/v) in ready to serve Mango beverages using 20 kHz ultrasound of power input 21 W, 32 W, 43 W for varying times 3, 5, 7 min to create emulsion droplets size 100-800 nm. Oil was extracted by ultrasound-assisted extraction and solvent extraction method by varying solvents, time, ultrasonic intensity etc. and physio-chemical characterization was conducted. Fatty acid profiling revealed that linolenic acid and linoleic acid are major fatty acids present in extracted oil. Effect of flaxseed oil in fruit-based beverage was evaluated in terms of turbidity, pH, acidity, color, antioxidant activity and carotenoids content. Pectin stabilizes emulsion droplets by generating electrostatic repulsion (ζ potential - 25 mV) and the emulsions were stable upto 18 days at (4 ± 2) °C. The rheological behaviour in terms of viscosity of the emulsion stayed unaffected with sonication time upon storage. The carotenoids and antioxidant activity significantly increased from 151.37 to 292.24 µg/mL and 26.99% to 61.43% respectively at 0 to 0.75% added oil in the beverage and enhanced stability by preventing lipid oxidation. Organoleptic score of 0.25% and 0.50% of the flaxseed oil in the beverage was found to be acceptable.

Sunflower protein isolates-composition, extraction and functional properties.

The demand of proteins from both plant and animal origin is growing throughout the world. But the proteins obtained from animals have an environmental effect and are expensive in market. Sunflower seeds are particularly intriguing among plant proteins since they are widely available have high protein content, nutritive value and functionalities. The quantity of anti-nutritional components in sunflower has been shown very low as compared to other plant sources. The governing factor which decides the applicability of sunflower proteins is its functional properties despite the absence of anti-nutritional compounds. The emulsification properties are comparable to soy protein isolates which increases the use of sunflower protein. However, foaming and gelling properties are not promising. But these properties can be improved by modification of proteins and eventually can be used for specific applications.

Role of Cissus quadrangularis in the Management of Osteoporosis: An Overview.

This article aims to provide a comprehensive review of Cissus quadrangularis (CQ), which is a traditional medicinal herb and has a potential osteoprotective effect. CQ is a perennial climber of family Vitaceae that is commonly found in the hotter parts of India. It is most widely used in India for improving bone health and is well known as "hadjod." It shows an anti-osteoporotic effect through different pathways mechanisms. It is natural matrices of excellence with proven bioactivity. Several cell line and animal studies demonstrated its protective nature against many diseases such as osteoporosis, arthritis, gastric ulcers etc. This review also highlights the phytochemicals identified to the date and related pharmacological applications. The discussion has also expanded to its oral formulations, which has been proven for its efficacy practically. However, the scientific information of CQ is not in the proper documentation for reference, and so availability of scientific knowledge of this climber is limited. Therefore, this review might be provided a platform to those who will be interested in studying further this herb, either for analyzing phytochemical profiling or its anti-osteoporotic usage. This is a crucial platform as several productive results have been reported on this herb, which likely to be beneficial for new drug discovery in future. Here we also discuss the bone remodeling and related factors influenced by the intake of CQ.

Characterization and cytotoxic activity of pigment extracted from Rhodotorula mucilaginosa to assess its potential as bio-functional additive in confectionary products.

The objective of the present investigation was to identify and characterize the pigment produced by yeast strain Rhodotorula mucilaginosa (MTCC-1403) using food industry residues. Onion peel powder and Mung bean husks were explored as substrate for submerged fermentation at previously optimized conditions in 3-L bioreactor. The pigment extraction was followed by quantification and characterization in terms of UV-visible spectroscopy, Fourier transform infrared spectroscopy, high performance liquid chromatography and fluorescence spectroscopy. Anti-carcinogenic activity of extracted pigment was measured against MCF-7 breast cancer cells. Furthermore, the pigment was used for the development of confectionary products (hard boiled candy and jelly) at different concentrations to evaluate its influence on bioactive properties and functionality. UV-visible spectroscopic reports revealed that torularhodin, ß-carotene, and torulene were major carotenoids present. In case of anti-carcinogenic activity, cell inhibition of 21.21% was observed with 40 µg of the extracted pigment after 72 h of incubation against MCF-7 cells. Significant influence of extracted pigment on confectionary products was observed for antioxidant activity, carotenoid content, color profile and sensory evaluation.

Biodegradable composite films/coatings of modified corn starch/gelatin for shelf life improvement of cucumber.

Composite films comprising modified corn starch and gelatin were developed using a solvent casting technique. The effect of varied content of citric acid and gelatin on the functional properties of the corn starch and composite films, respectively, was investigated. Modified corn starch films enriched with 5% citric acid presented excellent film properties like solubility, swelling index, water vapor permeability, and mechanical property as compared to other films. Similarly, composite films enriched with a ratio (4:1) also exhibited excellent film properties like mechanical and opacity than other composite films. FTIR results confirmed the cross linking among the modified corn starch chains, which is responsible for the enhancement in the film properties. From SEM results, it was noticed that the modified corn starch films exhibited slightly rougher, less shiny than the composite films. Efficacy of the composite solution as a coating formulation in terms of the quality and shelf life of the cucumber investigated. From the results, it was noted that the composite film forming solution as a coating formulation has the potential to extend the shelf-life of the cucumbers additionally up to 16 days.

Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.

Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process. The size of the CNF was in the range of 6.33-58.77 nm. Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%. Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%). Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied. Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days. Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments. Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp. when the cells of these organisms were spiked onto grapes and the samples were stored either at (25 ± 2) °C or (4 ± 1) °C for 28 days. The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.

Optimization of carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in bioreactor: A statistical approach.

Bio-colorants are preferred over synthetic colors as bio-colorants not only impart characteristic color to the food also contain harmless bio-active antioxidant nutrients. The present study was undertaken to investigate the potential of agro-industrial waste (Onion peels, potato skin, mung bean husk and pea pods) for carotenoid production from Rhodotorula mucilaginosa. After screening of appropriate carbon, nitrogen sources from agro-industrial waste, the fermentation conditions (pH, temperature, agitation) were optimized using Response Surface Methodology and optimum conditions were pH 6.1, temperature 25.8 ᴼC and agitation 119.6 rpm. Further, to evaluate the effect of aeration on carotenoids synthesis, fermentation was carried out in 3 L bio-reactor under optimum conditions with an air input of 1.0 vvm. Aeration causes elevation of more than 100 µg carotenoids per g of dry biomass. LC-MS of extracted pigment confirmed the presence of some other carotenoids along with ß-carotene. The major carotenoid compounds were found from the investigation were torularhodin, ß-carotene, and torulene.

Moth bean starch (Vigna aconitifolia): isolation, characterization, and development of edible/biodegradable films.

In the current work, moth bean starch was separated from the moth bean seeds which were cultivated in the semi arid regions of Haryana and Rajasthan, India using alkali treatment technique and characterized. Isolated and purified moth bean starch comprised (12.89-20.37%) amylose, 0.8% protein, 0.4% ash, swelling index and solubility were 10.8-14.7% and 6.4-9.8% respectively. For wrapping application, starch was modified using citric acid as cross linking agent (1-7% w/w of total starch) and film was made using casting method, and investigated the influence of citric acid on the functional properties of the films such as moisture content, solubility, swelling index, water vapor permeability and mechanical property. Significant difference in the functional properties among native and modified moth bean starch films was noticed. Interactions among starch chains due to cross linking in the modified starch films were reported using FTIR spectra. Surface micrographs of both purfied starch granules and films were studied using scanning electron microscopy. From the outcomes, it was exhibited that obtained starch granules has range large to small size with distorted cylinder and oval shapes. While cross linked starch films showed compact, slightly rough and homogeneous surface. Tested modified moth bean starch films containing citric acid (CA) and sorbitol were utilized as wrapping films to improve the shelf life of fresh lemon. Outcomes showed that the film contains 5% CA could be most promising wrapping materials for lemon that enhanced the shelf life of lemon additionally up to 12 days.

Beta-carotene-Encapsulated Solid Lipid Nanoparticles (BC-SLNs) as Promising Vehicle for Cancer: an Investigative Assessment.

Beta-carotene (BC), a red-colored pigment found in plants and animals, is one of the most extensively investigated carotenoids due to its provitamin-A, antioxidant, and anticancer properties. The anticancer activity of BC through oral administration is severely affected due to its low bioavailability and oxidative degradation. The present study aimed to formulate and characterize solid lipid nanoparticles (SLNs) of BC for enhanced bioavailability and therapeutic efficacy. Beta-carotene-loaded solid lipid nanoparticles (BC-SLNs) were prepared employing different combinations of glyceryl monostearate and gelucire. The characterization studies were performed for particle size, morphology, release behavior, and stability. BC-SLNs were also studied for in vitro cytotoxicity in human breast cancer cell lines (MCF-7) and pharmacokinetic studies in Wistar rats. The cytotoxicity studies confirmed that encapsulation of BC within the lipid bilayers of nanoparticles did not affect its anticancer efficacy. An improved anticancer activity was observed in BC-SLNs as compared to the free BC. BC-SLNs enhanced the bioavailability of BC on oral administration by sustaining its release from the lipid core and prolongation of circulation time in the body. Similarly, area under the curve (AUCtotal) enhanced 1.92-times more when BC was incorporated into SLNs as compared to free BC. In conclusion, solid lipid nanoparticles could be an effective and promising strategy to improve the biopharmaceutical properties of carotenoids for anticancer effects.

Effect of chitosan on physicochemical and rheological attributes of bread.

The aim of this study was to develop fiber-enriched functional bread containing selected concentration of chitosan (0.1%, 0.5%, 1%, 1.5%, and 2% w/w). Dynamic rheology of bread dough established that viscous moduli were lower than elastic moduli. Until 1% chitosan concentration, dough extensibility increased. Avrami nonlinear regression equation was selected as a convenient mathematical tool to model bread crumb staling kinetics. Scanning electron microscopy, Fourier-transform infrared, differential scanning calorimetry, and extensibility tests were used to examine the changes of the structure of control dough and chitosan containing dough. Specific volume of bread was inversely proportional to firmness of bread. Firmness is directly proportional function of storage time and chitosan quantity. Sensory evaluation revealed that 1% chitosan can be used as potential functional ingredient to prevent chronic diseases like obesity, type 2 diabetes, constipation, and colon cancer.

Lycopene loaded whey protein isolate nanoparticles: An innovative endeavor for enhanced bioavailability of lycopene and anti-cancer activity.

The work entails a novel strategy of formulating the lycopene loaded whey protein isolate nanoparticles (LYC-WPI-NPs) solely using the rational blend of biomacromolecule without using equipment-intensive techniques. The LYC-WPI-NPs were fabricated as a substantial drug delivery platform, with maximum entrapment, spatial and controlled release manners, exceptional plasma concentration, and perspective for discrepancy delivery of therapeutics. Prepared nano-formulations were measured in ultra-fine size (100-350 nm) with sphere-shaped. The percent lycopene entrapment of prepared LYC-WPI-NPs was estimated in the range to 50 and 65%. In vitro percent cumulative release study demonstrated deaden and extended release i.e. approximately 75% following 16th h. The in vitro percent cell survival (cytotoxicity study) of prepared nanoparticles was evaluated against MCF-7 breast cancer cells by MTT based colorimetric assay. Sub-cellular localization of lycopene when delivered by LYC-WPI-NPs was assessed by HPLC (high performance liquid chromatography). The WPI-NPs enhance the oral bioavailability of lycopene by controlling its release from nano-formulation and facilitating its absorption through lymphatic pathways. Prophylactic anticancer efficacy of LYC-WPI-NPs was evaluated thereafter on experimentally induced breast cancer animal model. Conclusively, it may quite reasonable that lycopene loaded protein nanoparticles are competent to improve the biopharmaceutical attributes of lycopene and demonstrated prophylactic anticancer activity, decrease tumor proliferation and increase the survival rate of treated animals, thus signifying their feasible usefulness in cancer therapeutic and intervention.

Beta carotene-loaded zein nanoparticles to improve the biopharmaceutical attributes and to abolish the toxicity of methotrexate: a preclinical study for breast cancer.

Beta carotene (ßC) loaded nanoparticles of zein (ßC-NPs) were developed using modified phase separation technique. ßC-NPs were prepared using different zein concentration and optimized formulation was selected on the basis of micromeritics properties and entrapment efficiency. Further, ßC-NPs were evaluated for in vitro release, in vitro cell-survival, cellular localization and apoptosis induced in MCF-7 cells. The combined effect of the ßC and its nanoparticulate counterpart with MTX was evaluated thereafter for cytotoxicity and apoptotic activity in MCF-7 cells. In comparison to free ßC, the ßC-NPs demonstrated noteworthy improvement in various biopharmaceutical attributes viz Cmax (∼2.3-folds), AUCtotal (2.7-folds), t1/2 (∼1.5 folds) and MRT (∼1.5 folds), further indicating the remarkable increment in oral bioavailability of ßC after incorporation in zein nanoparticles. The anti-tumour potential of prepared ßC-NPs and effects of free ßC and ßC-NPs were investigated upon anticancer efficacy of methotrexate (MTX) in experimentally induced breast cancer rat model. Protective role of ßC on MTX-associated hepatic toxicity in wistar rats was also determined using haematological and histopathological approaches. In a nutshell, zein nanoparticles improved the cellular uptake, cytotoxicity and exhibited enhanced oral biopharmaceutical performance of ßC. This combination regimen could also be promising platform to facilitate the therapeutic benefits of anticancer agents.

Recent advances in galactose-engineered nanocarriers for the site-specific delivery of siRNA and anticancer drugs.

Galactosylated nanocarriers have recently emerged as viable and versatile tools to deliver drugs at an optimal rate specifically to their target tissues or cells, thus maximizing their therapeutic benefits while circumventing off-target effects. The abundance of lectin receptors on cell surfaces makes the galactosylated carriers suitable for the targeted delivery of bioactives. Additionally, tethering of galactose (GAL) to various carriers, including micelles, liposomes, and nanoparticles (NPs), might also be appropriate for drug delivery. Here, we review recent advances in the development of galactosylated nanocarriers for active tumor targeting. We also provide a brief overview of the targeting mechanisms and cell receptor theory involved in the ligand-receptor-mediated delivery of drug carriers.

Methotrexate and beta-carotene loaded-lipid polymer hybrid nanoparticles: a preclinical study for breast cancer.

AIM: This work was intended to investigate the targeting potential of fructose-tethered lipid-polymeric hybrid nanoparticles (F-BC-MTX-LPHNPs) co-loaded with beta carotene (BC) and methotrexate (MTX) in breast cancer therapeutics and find out the possible protective role of BC on MTX-induced toxicity. MATERIALS & METHODS: F-BC-MTX-LPHNPs were fabricated using self-assembled nano-precipitation technique. Fructose was conjugated on the surface of the particles. The in vitro cytotoxicity, sub-cellular localization and apoptotic activity of F-BC-MTX-LPHNPs were evaluated against MCF-7 breast cancer cells. The antitumor potential of F-BC-MTX-LPHNPs was further studied. RESULTS & CONCLUSION: Outcomes suggested that F-BC-MTX-LPHNPs induced the highest apoptosis index (0.89) against MCF-7 cells. Following 30 days of treatment, the residual tumor progression was assessed to be approximately 32%, in animals treated with F-BC-MTX-LPHNPs. F-BC-MTX-LPHNPs are competent to selectively convey the chemotherapeutic agent to the breast cancers. Beta carotene ameliorated MTX-induced hepatic and renal toxicity.

Microencapsulation of ß-Carotene Based on Casein/Guar Gum Blend Using Zeta Potential-Yield Stress Phenomenon: an Approach to Enhance Photo-stability and Retention of Functionality.

ß-Carotene, abundant majorly in carrot, pink guava yams, spinach, kale, sweet potato, and palm oil, is an important nutrient for human health due to its scavenging action upon reactive free radicals wherever produced in the body. Inclusion of liposoluble ß-carotene in foods and food ingredients is a challenging aspect due to its labile nature and low absorption from natural sources. This fact has led to the application of encapsulation of ß-carotene to improve stability and bioavailability. The present work was aimed to fabricate microcapsules (MCs) of ß-carotene oily dispersion using the complex coacervation technique with casein (CA) and guar gum (GG) blend. The ratio of CA:GG was found to be 1:0.5 (w/v) when optimized on the basis of zeta potential-yield stress phenomenon. These possessed a higher percentage yield (71.34 ± 0.55%), lower particle size (176.47 ± 4.65 µm), higher encapsulation efficiency (65.95 ± 5.33%), and in general, a uniform surface morphology was observed with particles showing optimized release behavior. Prepared MCs manifested effective and controlled release (up to 98%) following zero-order kinetics which was adequately explained by the Korseymer-Peppas model. The stability of the freeze-dried MCs was established in simulated gastrointestinal fluids (SGF, SIF) for 8 h. Antioxidant activity of the MCs was studied and revealed the retention of the functional architecture of ß-carotene in freeze-dried MCs. Minimal photolytic degradation upon encapsulation of ß-carotene addressed the challenge regarding photo-stability of ß-carotene as confirmed via mass spectroscopy.

Fabrication and functional attributes of lipidic nanoconstructs of lycopene: An innovative endeavour for enhanced cytotoxicity in MCF-7 breast cancer cells.

The present study investigates the anticancer efficacy of lycopene loaded lipidic nanostructured particles. With a homogenization-evaporation technique, lycopene loaded solid lipid nanoparticles (LYC-SLNs) were fabricated with different ratios of biocompatible viz. compritol ATO 888 and gelucire, and evaluated for their micromeretics properties, in vitro release, in vitro cytotoxicity, cellular uptake and apoptosis induced in MCF-7 cells. Effects of anticancer potential of LYC-SLNs on the efficacy of methotrexate (MTX), a well-established anticancer agent, was evaluated thereafter. Cell culture experiments revealed a considerably higher cellular uptake of LYC-SLNs in MCF-7 cells, as compared to the free LYC. The concentration and time dependent cell survival of MCF-7 cells was significantly reduced by LYC-SLNs, as compared to their free LYC counterparts. Additionally, the combined cytotoxicity of the LYC and its nanostructured formulation with MTX was evaluated. The results of cytotoxicity and apoptotic study revealed synergism at all-time points up to 48h. In a nutshell, the combination regimen could be promising approach to improve the therapeutic benefits of anticancer agents.

Formation and functional attributes of electrostatic complexes involving casein and anionic polysaccharides: An approach to enhance oral absorption of lycopene in rats in vivo.

The current work entails a novel strategy of formulating the microparticles of lycopene solely using rational blends of biopolymers without using equipment-intensive techniques. The study is intended to enhance oral bioavailability of lycopene by controlling its release from micro-formulation and facilitating its absorption though lymphatic pathways. Considering the minimum particle size, maximum entrapment efficiency and loading capacity, the amounts of casein (i.e., protein) and gum tragacanth (i.e., polysaccharide) were selected as the critical factors for formulation of microparticles. Complex formation and electrostatic interaction was confirmed by Fourier transform infra red (FTIR) spectra. Size and surface properties of microparticles were studied using scanning electron microscopy (SEM). The optimized formulation (mean particle size: ∼130µm; % entrapment efficiency: ∼67% and loading capacity: ∼71%) designated noticeable improvement in lycopene release profile (over 80% in 24h). Increment in the values of Cmax (2.22-fold) and AUC (1.97-fold) further indicated noteworthy augmentation in the rate and extent of bioavailability by the microparticles formulation compared to plain lycopene. The resulting formulation was found to be quite stable all through two months of study episode. The resultant microparticles formulation was evaluated for antioxidant activity and tested for their effectiveness in self life enhancement of vegetable oil by calculating peroxide value under temperature and storage condition. Encapsulation strongly increased the stability of micronutrients. The current investigations, therefore, report the successful development of biopolymeric microparticles with improved bioavailability potential of lycopene.