ABSTRACT
OBJECTIVE: To determine the frequency of antiphospholipid antibodies (aPL) in patients with unexplained articular manifestations. MATERIAL AND METHODS: Three hundred thirteen patients suffering from arthritis or arthralgia without evident cause and 266 healthy blood donors (HBD) were included in the study. Anticardiolipin antibodies (aCL) and anti-beta 2-glycoprotein I antibodies (aß2GPI) were measured by ELISA. RESULT: Out of the 313 patients, 250 were females and 63 were males. The mean age of patients was 49 ± 14 years (17-87 years). One hundred eleven patients have arthralgia and 202 have arthritis. The frequency of aCL and/or aß2 GPI (24.9%) was significantly higher in patients than in HBD (10.9%). The frequency of aß2GPI was 23.6% in patients and 9.4% in the control group (p < 10-3 ). aß2GPI-IgA was significantly more frequent in patients than in the control group (20.4% vs. 7.5%, p < 10-3 ). aß2GPI was most commonly observed than aCL in patients (23.6% vs. 6.4%, p < 10-6 ). IgA isotype of aß2GPI was the most frequent in 20.4% of patients while IgG and IgM were detected in 5.4% and 2.9% respectively. CONCLUSION: This study showed that aPL were common in patients with articular manifestations and were mainly directed against ß2 GPI. The role of these antibodies remains to be specified.
Subject(s)
Antiphospholipid Syndrome , Arthritis , Male , Female , Humans , Adult , Middle Aged , Antibodies, Antiphospholipid , Antibodies, Anticardiolipin , Autoantibodies , beta 2-Glycoprotein I , Arthritis/epidemiology , Immunoglobulin A , ArthralgiaABSTRACT
BACKGROUND: Antiphospholipid (aPL) antibodies have been reported in several autoimmune diseases. The aim of this study was to evaluate the frequency of aPL (anti-cardiolipin antibodies (aCL) and anti-ß2 glycoprotein I antibodies (aß2GPI)) in patients with autoimmune thyroid diseases (AITD). METHODS: One hundred and ninety-five patients with AITD (139 Hashimoto's thyroiditis (HT) patients and 56 Graves' disease (GD) patients) and 90 healthy blood donors (HBD) were studied. IgG, IgA and IgM aCL and aß2GPI were determined by ELISA. RESULTS: One hundred fifty-four AITD patients were women and 41 were men. Fifty-six healthy subjects were women and 34 were men. The median age of patients and the control group was 45 and 38.5 years, respectively. The frequency of aPL was significantly higher in patients with AITD and in patients with HT than in HBD (33.3% vs 11.1%, p < 10-3 and 38.1% vs 11.1%, p < 10-3 ). The frequency of aPL in GD was significantly lower than in HT (21.4% vs 38.1%, p = 0.025). In patients with HT, aß2GPI (34.5%) was significantly more frequent than aCL (13.6%) (p < 10-3 ). The frequency of aß2GPI was significantly higher in patients with HT than in healthy population (34.5% vs 11.1%, p < 10-3 ). In HT patients, IgA isotype of aß2GPI was significantly more common than in HBD and in GD patients (27.3% vs 7.8%, p < 10-3 and 27.3% vs 12.5%, p = 0.02, respectively). CONCLUSION: aß2GPI and not aCL were frequent in AITD. IgA was the predominant isotype of aß2GPI. aß2GPI-IgA was more frequent in HT than in GD.
Subject(s)
Antibodies, Antiphospholipid , Hashimoto Disease , Male , Humans , Female , beta 2-Glycoprotein I , Antibodies, Anticardiolipin , Hashimoto Disease/epidemiology , Immunoglobulin AABSTRACT
AIM: To determine the frequency of serological markers of RA in patients with anti-ß2 glycoprotein I antibodies (aß2GPI) of IgA isotype. MATERIAL AND METHODS: A retrospective study was conducted on 67 patients with aß2GPI-IgA. Ninety healthy blood donors (HBD) were used as a control group. IgG anti-cyclic citrullinated peptides antibodies (CCP-Ab) and rheumatoid factors (RF) IgG, IgA, and IgM were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: Seventeen patients and eight HBD had CCP-Ab and/or RF (25.4% vs. 8.9%, p = 0.005, CI 95% [14.95; 35.79], odds ratio = 3.5). The frequency of CCP-Ab was significantly higher in patients than in healthy subjects (14.9% vs. 3.3%, p = 0.009). IgA isotype of RF was significantly higher in patients than in controls (7.5% vs. 0%, p = 0.02). In male patients, CCP-Ab and/or RF were more frequent than in healthy male subjects (37.5% vs. 11.8%, p = 0.02). In patients, no correlation was found between the levels of aß2GPI-IgA and CCP-Ab (r = 0.082, p = 0.51). There was no correlation between the level aß2GPI-IgA and the level of the isotypes of RF (IgG, IgA, and IgM) in patients (r = 0.1, p = 0.37; r = 0.17, p = 0.17 and r = 0.07, p = 0.59 respectively). CONCLUSION: Frequencies of CCP-Ab and RF are high in patients with aß2GPI-IgA suggesting that these patients are susceptible to developing RA.
Subject(s)
Arthritis, Rheumatoid , Immunoglobulin A , Anti-Citrullinated Protein Antibodies , Autoantibodies , Biomarkers , Glycoproteins , Humans , Immunoglobulin G , Immunoglobulin M , Male , Peptides, Cyclic , Retrospective Studies , Rheumatoid FactorABSTRACT
OBJECTIVE: This study was conducted to evaluate the frequency of anti-Saccharomyces cerevisiae antibodies (ASCA) in patients with rheumatoid arthritis (RA). METHODS: Eighty-three RA patients with positive anti-cyclic citrullinated antibodies (anti-CCP) and 160 healthy blood donors were included in this study. ASCA IgG and IgA were assessed with enzyme-linked immunosorbent assay. RESULTS: The frequency of ASCA was significantly higher in RA patients than in healthy subjects (22.9% vs 3.7%, Pâ <â 10-3). Both ASCA IgG and ASCA IgA were significantly more frequent in RA patients than in the control group (20.5% vs 3.1%, Pâ <â 10-3and 9.6% vs 0.6%, Pâ =â .002, respectively). ASCA IgG and ASCA IgA levels were significantly higher in RA patients than in healthy subjects (7.8â ±â 8.4 U/mL vs 2.3â ±â 2.8 U/mL, Pâ <â 10-6 and 6.2â ±â 10.9 U/mL vs 3.4â ±â 1.7 U/mL, Pâ =â .002, respectively). CONCLUSION: A high frequency of ASCA IgG and ASCA IgA has been found in RA patients.
