High frequency of lamivudine resistance mutations in Brazilian patients co-infected with HIV and hepatitis B.

This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood samples for HBsAg detection. The load of HBV was determined using an "in-house" real-time polymerase chain reaction. HBV genotypes/subgenotypes, antiviral resistance, basal core promoter (BCP), and precore mutations were detected by DNA sequencing. Twenty-eight patients with co-infection were identified. The distribution of HBV genotypes among these patients was A (n = 9; 50%), D (n = 4; 22.2%), G (n = 3; 16.7%), and F (n = 2; 11.1%). Eighteen patients were treated with LAM and six patients were treated with LAM plus TDF. The length of exposure to LAM and TDF varied from 4 to 216 months. LAM resistance substitutions (rtL180M + rtM204V) were detected in 10 (50%) of the 20 patients with viremia. This pattern and an accompanying rtV173L mutation was found in four patients. Three patients with the triple polymerase substitution pattern (rtV173L + rtL180M + rtM204V) had associated changes in the envelope gene (sE164D + sI195M). Mutations in the BCP region (A1762T, G1764A) and in the precore region (G1896A, G1899A) were also found. No putative TDF resistance substitution was detected. The data suggest that prolonged LAM use is associated with the emergence of particular changes in the HBV genome, including substitutions that may elicit a vaccine escape phenotype. No putative TDF resistance change was detected after prolonged use of TDF.

Genotypic distribution of hepatitis C among hepatitis C and HIV co-infected patients in Brazil.

Information on hepatitis C virus (HCV) genotypic distribution among HIV-HCV co-infected patients is lacking in Brazil as well as other Latin American countries. The objective of this study was to evaluate the level of exposure to different risk factors associated with HCV transmission among a group of co-infected patients and to characterize the genotypic distribution of HCV in this cluster. A series of 100 HIV-HCV co-infected patients was analysed. The data to be analysed were collected from specific laboratory tests. Information was collected through a questionnaire. HCV genotyping was carried out by sequencing the 5' non-coding region of HCV. Chi-square and Fischer association tests or Kruskal-Wallis test were used to study the association between HCV transmission-related variables and the established genotypes. In conclusion, exposure to multiple risk factors associated with HCV transmission was common among HIV co-infected patients and an association between HCV genotype 3 and intravenous drug user was observed.

[Physical aggression and social class]. / Agressão física e classe social.

OBJECTIVE: Considering the increase of violence and the scarcity of informations about the relation between social class and victimization by physical aggression, a study was conducted to investigate this association. METHODS: A hospital-based case-control study. Cases and controls were recruited at a hospital, first-aid clinic, from 1/10/93 to 19/1/95. The study included 191 cases and 222 controls selected from among patients with non-violent clinical-surgical complaints, frequency-matched to cases by sex and age. Using a standardized questionnaire applied by trained interviewers, information obtained included social class, skin color, marital status, smoking habits, alcohol consumption and illicit drug use. RESULTS: Adjusting for sex and age, the risk of victimization by physical aggression was significantly higher for the subproletariat, Odds Ratio (OR) 4.20, 95% Confidence Interval (95% CI) 1.99-8.84; single (OR = 2.10) or informal union (OR = 2.62) as marital status (reference group = married); smokers of more than 10 cigarettes/day (OR = 2.75); alcohol consumption (OR = 2.08 for < or = 240 grams/week and OR = 24.05 for > 240 grams/week); and illicit drug users (OR = 3.07). After adjusting for all factors studied a significant risk remained for the subproletariat (OR = 3.28, 95% CI 1.42-7.59); single as marital status (OR = 2.05, 95% CI 1.09-3.88); and alcohol consumption (OR = 2.01, 95% IC 1.07-3.77 for < or = 240 and OR = 15.93, 95% CI 5.09-49.8 for > 240 grams/week) CONCLUSION: Social class is an important factor in the phenomenon of victimization by physical aggression, with the subproletariat deserving special attention in the strategies of intervention regarding this problem.