ABSTRACT
Promising initial results from the use of membrane-fractionated extracts of tomato leaf as crop protection agents have recently been reported. This paper provides additional evidence from larger scale experiments that identify an efficient pipeline for the separation of tomato leaf extracts to generate a fraction with significant defence elicitor activity. A UF tubular membrane 150 kDa, with an internal diameter of 5 mm, proved appropriate for initial extract clarification, whereas afterwards a UF 10 kDa and three NF membranes (200-800 Da) in sequence were evaluated for the subsequent fractionation of this tomato extract. The compositions of sugars, proteins and total biophenols were changed in these fractions with respect to the initial extract. The initial extract ratio of sugars: proteins: biophenols was 1:0.047:0.052, whereas for the retentate of the 800 Da NF membrane, which has the higher crop protection activity, this ratio was 1:0.06:0.1. In this regard, it appears that the main crop protection effect in this fraction was due to the sugars isolated. It was found that with the appropriate membrane cascade selection (UF 150 kDa, UF 10 kDa and NF 800 Da) it was possible to produce (easily and without the need of additional chemicals) a fraction that has significant activity as an elicitor of disease resistance in tomato, whereas the remaining fractions could be used for other purposes in a biorefinery. This is very promising for the wider application of the proposed approach for the relatively easy formulation of bio-based aqueous streams with bio-pesticide activities.
ABSTRACT
Patras Gulf pockmark field (Western Greece) is a tectonically controlled field that has been activated at least twice by strong earthquakes (M5.4, 14 July 1993 and M6.4, 8 June 2008), and episodic gas seepages have been recorded in the past using geophysical means. A distributed temperature sensor (DTS) system was deployed inside a shallow pockmark and along an active fault at the northern end of the field. This ongoing experiment represents the first long-term monitoring ever conducted on gas-bearing pockmarks and active faults by the DTS system. For now, we have acquired and analyzed data regarding about 1.56 years. One of the primary objectives of this study is to establish methodological queues for data processing and analysis, including spectral analysis and incomplete data treatment techniques, to be standardized for use in further stages of the experiment. Spectral analysis was proven capable of separating the temperature footprint of background environmental components, such as sea-atmosphere heat flux, tides, and winds/waves, from high-frequency temperature residuals. Those residuals represent unusual events that might be correlated to seismicity. Monitoring the causal relationship between seismic activity and seabed water temperature changes in the field was thus attempted. No significant local earthquakes occurred during the monitoring period. Although the relation between seismicity and irregular seabed water temperature events was not systematic, we postulate that four thermal events have a causative link with the local seismicity. The DTS system constitutes a low-cost monitoring system, and the promising preliminary results of this experiment suggest that it is worth testing for a longer period.
ABSTRACT
Tomato leaves have been shown to contain significant amounts of important metabolites involved in protection against abiotic and biotic stress and/or possessing important therapeutic properties. In this work, a systematic study was carried out to evaluate the potential of a sustainable process for the fractionation of major biomolecules from tomato leaves, by combining aqueous extraction and membrane processes. The extraction parameters (temperature, pH, and liquid/solid ratio (L/S)) were optimized to obtain high amounts of biomolecules (proteins, carbohydrates, biophenols). Subsequently, the aqueous extract was processed by membrane processes, using 30-50 kDa and 1-5 kDa membranes for the first and second stage, respectively. The permeate from the first stage, which was used to remove proteins from the aqueous extract, was further fractionated in the second stage, where the appropriate membrane material was also selected. Of all the membranes tested in the first stage, regenerated cellulose membranes (RC) showed the best performance in terms of higher rejection of proteins (85%) and lower fouling index (less than 15% compared to 80% of the other membranes tested), indicating that they are suitable for fractionation of proteins from biophenols and carbohydrates. In the second stage, the best results were obtained by using polyethersulfone (PES) membranes with an NMWCO of 5 kDa, since the greatest difference between the rejection coefficients of carbohydrates and phenolic compounds was obtained. In vivo bioactivity tests confirmed that fractions obtained with PES 5 kDa membranes were able to induce plant defense against P. syringae.
