Isolation and characterization of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) from milk of dairy goats under low-input farm management in Greece.

The presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk is a challenge for veterinarians and public health professionals. In this study, we investigated the presence and clonality of S. aureus and MRSA in milk of individual dairy goats with subclinical mastitis reared under the low-input farming system in Greece and determined the isolates' enterotoxin gene carriage and their ability to form biofilms. S. aureus was isolated from 162 out of the 559 milk samples examined (29 %) and one isolate per S. aureus-positive sample was further characterized. S. aureus isolates were very closely related even among farms of distant geographical regions. Nine S. aureus isolates carried a functional mecA gene and were classified as MRSA. The S. aureus protein A (spa) typing in the MRSA isolates showed that four belonged to spa type t127 (44.4 %), three to t2049 (33.3 %) and two to t7947 (22.2 %). The spa type t7947 is reported for the first time in Greece. The MRSA isolates originated from two very distantly located farms, one located in the island of Skopelos and the other in Central Macedonia. Four of the MRSA isolates carried the staphylococcal enterotoxin genes sea or sec. Most of the isolates (92 % of S. aureus and 77.8 % of the MRSA) possessed moderate or weak biofilm-formation ability. Raw milk from low-input goat herds may serve as a potential vector of antimicrobial-resistant S. aureus to raw-milk consumers.

Bacterial subclinical mastitis and its effect on milk quality traits in low-input dairy goat herds.

The objective of the study was to investigate and quantify the effects of subclinical mastitis (SCM) on the gross chemical composition of milk in low-input dairy goat herds. Dairy goats (n=590) of two native Greek breeds from four representative low-input farms were randomly selected and used in the study. Α prospective study was conducted, including monthly monitoring and milk sampling of the same individual goats during the course of two consecutive milking periods. Mixed linear regression models were built to assess how the chemical composition of milk was affected by (1) SCM and (2) the different pathogens isolated from SCM cases. Goats with SCM had lower milk-fat content (MFC), daily milk-fat yield (DMFY), milk-lactose content (MLC) and daily milk-lactose yield (DMLY), and slightly higher milk-protein content (MPC) and daily milk-protein yield (DMPY), compared with goats without SCM. Milk produced by goats with SCM due to coagulase-positive staphylococci and Mycoplasma agalactiae had significantly lower MFC, DMFY, MLC and DMLY, and higher MPC and DMPY, compared with the milk produced by healthy goats. Finally, goats with SCM due to coagulase-negative staphylococci had lower DMFY, MLC and DMLY and higher DMPY compared with the healthy ones.