ABSTRACT
In this study, the fate of Alicyclobacillus acidoterrestris spores in different types of juice concentrates stored under different conditions was investigated. In addition, the impact of dilution procedures during the enrichment step for the detection of Alicyclobacillus in lemon juice concentrates was studied. Pear, red grape, mango, tangerine, carrot and lemon juice concentrates (50-69.4°Brix, pH1.7-4.3) were inoculated with A. acidoterrestris spores (10(3) spore/mL) and stored at 4 °C and 20 °C, after which the spores were counted at 0, 2, 5, 9, 17, 21, 28, 36, 43, and 50 days. No significant differences in the number of Alicyclobacillus spores were observed at storage temperatures of 4 °C and 20 °C (p>0.05). The results also indicated that the number of spores of A. acidoterrestris remained stable in all types of juice concentrates during the storage period, except in lemon juice concentrate. In lemon juice concentrate, a decline in A. acidoterrestris spore populations of 0.3-0.8 log CFU/mL was observed within 5-10 days of storage. The decline in A. acidoterrestris spore populations was more pronounced in cloudy lemon juice concentrate, which contained higher concentrations of flavonoids (mainly eriocitrin and hesperidin) than clarified lemon juice concentrate. It was also found that dilution of lemon juice concentrate samples in the proportion of 1:19 allowed the germination of A. acidoterrestris spores and the growth of populations of up to 10(7) CFU/mL. In contrast, the proportion (1:9) recommended in internationally recognized methods led to a reduction in the population of this microorganism that would yield false negative results. Data presented in this study demonstrated that Alicyclobacillus spores remain stable in most juice concentrates during storage, but that natural antimicrobial compounds present in some of them may decrease spore counts and inhibit their recovery by detection procedures.
Subject(s)
Alicyclobacillus/physiology , Food Microbiology , Fruit and Vegetable Juices/microbiology , Alicyclobacillus/isolation & purification , Colony Count, Microbial , Food Handling/standards , Spores, Bacterial/isolation & purification , Temperature , Time FactorsABSTRACT
The aim of this study is to analyze the origin and quality of water used for consumption in a sample of households in Matanza-Riachuelo river basin area in Greater Buenos Aires, Argentina. The results of drinking water by source indicated that 9% of water samples from the public water system, 45% of bottled water samples and 80% of well water samples were not safe for drinking due to excess content of coliforms, Escherichia coli or nitrates. Individuals living in households where well water is the main source of drinking water have a 55% higher chance of suffering a water-borne disease; in the cases of diarrheas, the probability is 87% higher and in the case of dermatitis, 160% higher. The water for human consumption in this region should be provided by centralized sources that assure control over the quality of the water.
Subject(s)
Drinking Water/standards , Urban Health/statistics & numerical data , Water Quality , Water Supply/standards , Adolescent , Adult , Aged , Aged, 80 and over , Argentina , Child , Child, Preschool , Drinking Water/analysis , Drinking Water/chemistry , Drinking Water/microbiology , Escherichia coli/isolation & purification , Female , Health Surveys , Humans , Infant , Infant, Newborn , Male , Middle Aged , Nitrates/analysis , Rivers , Surveys and Questionnaires , Water Supply/analysis , Water Supply/statistics & numerical data , Young AdultABSTRACT
El objetivo del estudio es analizar el origen y la calidad del agua domiciliaria utilizada para el consumo, en una muestra de hogares del área de la cuenca Matanza-Riachuelo del Gran Buenos Aires, Argentina. Según su origen, el 9% de las muestras de agua provenientes de la red pública, el 45% de las de agua envasada y el 80% de las provenientes de perforaciones o pozos individuales resultaron no potables por exceso de coliformes, Escherichia coli o nitratos. Los individuos de la muestra de hogares en los que la fuente principal de agua para el consumo eran pozos individuales presentaron una probabilidad 55% superior de padecer alguna enfermedad de origen hídrico, probabilidad que llegaría al 87% en el caso de las diarreas y al 160% en el de las dermatitis. El agua para consumo humano en este territorio debería provenir de fuentes centralizadas que aseguren el control de la calidad del agua.
The aim of this study is to analyze the origin and quality of water used for consumption in a sample of households in Matanza-Riachuelo river basin area in Greater Buenos Aires, Argentina. The results of drinking water by source indicated that 9% of water samples from the public water system, 45% of bottled water samples and 80% of well water samples were not safe for drinking due to excess content of coliforms, Escherichia coli or nitrates. Individuals living in households where well water is the main source of drinking water have a 55% higher chance of suffering a water-borne disease; in the cases of diarrheas, the probability is 87% higher and in the case of dermatitis, 160% higher. The water for human consumption in this region should be provided by centralized sources that assure control over the quality of the water.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Drinking Water/standards , Urban Health/statistics & numerical data , Water Quality , Water Supply/standards , Argentina , Drinking Water/analysis , Drinking Water/chemistry , Drinking Water/microbiology , Escherichia coli/isolation & purification , Health Surveys , Nitrates/analysis , Surveys and Questionnaires , Rivers , Water Supply/analysis , Water Supply/statistics & numerical dataABSTRACT
El objetivo del estudio es analizar el origen y la calidad del agua domiciliaria utilizada para el consumo, en una muestra de hogares del área de la cuenca Matanza-Riachuelo del Gran Buenos Aires, Argentina. Según su origen, el 9% de las muestras de agua provenientes de la red pública, el 45% de las de agua envasada y el 80% de las provenientes de perforaciones o pozos individuales resultaron no potables por exceso de coliformes, Escherichia coli o nitratos. Los individuos de la muestra de hogares en los que la fuente principal de agua para el consumo eran pozos individuales presentaron una probabilidad 55% superior de padecer alguna enfermedad de origen hídrico, probabilidad que llegaría al 87% en el caso de las diarreas y al 160% en el de las dermatitis. El agua para consumo humano en este territorio debería provenir de fuentes centralizadas que aseguren el control de la calidad del agua.(AU)
The aim of this study is to analyze the origin and quality of water used for consumption in a sample of households in Matanza-Riachuelo river basin area in Greater Buenos Aires, Argentina. The results of drinking water by source indicated that 9% of water samples from the public water system, 45% of bottled water samples and 80% of well water samples were not safe for drinking due to excess content of coliforms, Escherichia coli or nitrates. Individuals living in households where well water is the main source of drinking water have a 55% higher chance of suffering a water-borne disease; in the cases of diarrheas, the probability is 87% higher and in the case of dermatitis, 160% higher. The water for human consumption in this region should be provided by centralized sources that assure control over the quality of the water.(AU)
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Drinking Water/standards , Urban Health/statistics & numerical data , Water Quality , Water Supply/standards , Argentina , Drinking Water/analysis , Drinking Water/chemistry , Drinking Water/microbiology , Escherichia coli/isolation & purification , Health Surveys , Nitrates/analysis , Surveys and Questionnaires , Rivers , Water Supply/analysis , Water Supply/statistics & numerical dataABSTRACT
The aim of this study is to analyze the origin and quality of water used for consumption in a sample of households in Matanza-Riachuelo river basin area in Greater Buenos Aires, Argentina. The results of drinking water by source indicated that 9
of water samples from the public water system, 45
of bottled water samples and 80
of well water samples were not safe for drinking due to excess content of coliforms, Escherichia coli or nitrates. Individuals living in households where well water is the main source of drinking water have a 55
higher chance of suffering a water-borne disease; in the cases of diarrheas, the probability is 87
higher and in the case of dermatitis, 160
higher. The water for human consumption in this region should be provided by centralized sources that assure control over the quality of the water.
