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1.
Phys Rev Lett ; 117(13): 136401, 2016 Sep 23.
Article in English | MEDLINE | ID: mdl-27715109

ABSTRACT

We report on optical reflectivity experiments performed on Cd_{3}As_{2} over a broad range of photon energies and magnetic fields. The observed response clearly indicates the presence of 3D massless charge carriers. The specific cyclotron resonance absorption in the quantum limit implies that we are probing massless Kane electrons rather than symmetry-protected 3D Dirac particles. The latter may appear at a smaller energy scale and are not directly observed in our infrared experiments.

3.
Phys Rev Lett ; 114(25): 256401, 2015 Jun 26.
Article in English | MEDLINE | ID: mdl-26197136

ABSTRACT

A three-dimensional strong-topological insulator or semimetal hosts topological surface states which are often said to be gapless so long as time-reversal symmetry is preserved. This narrative can be mistaken when surface state degeneracies occur away from time-reversal-invariant momenta. The mirror invariance of the system then becomes essential in protecting the existence of a surface Fermi surface. Here we show that such a case exists in the strong-topological-semimetal Bi(4)Se(3). Angle-resolved photoemission spectroscopy and ab initio calculations reveal partial gapping of surface bands on the Bi(2)Se(3) termination of Bi(4)Se(3)(111), where an 85 meV gap along Γ̅K̅ closes to zero toward the mirror-invariant Γ̅M̅ azimuth. The gap opening is attributed to an interband spin-orbit interaction that mixes states of opposite spin helicity.

4.
Sci Rep ; 4: 5168, 2014 Jun 04.
Article in English | MEDLINE | ID: mdl-24893841

ABSTRACT

We present an ARPES study of the surface states of Ru2Sn3, a new type of a strong 3D topological insulator (TI). In contrast to currently known 3D TIs, which display two-dimensional Dirac cones with linear isotropic dispersions crossing through one point in the surface Brillouin Zone (SBZ), the surface states on Ru2Sn3 are highly anisotropic, displaying an almost flat dispersion along certain high-symmetry directions. This results in quasi-one dimensional (1D) Dirac electronic states throughout the SBZ that we argue are inherited from features in the bulk electronic structure of Ru2Sn3 where the bulk conduction bands are highly anisotropic. Unlike previous experimentally characterized TIs, the topological surface states of Ru2Sn3 are the result of a d-p band inversion rather than an s-p band inversion. The observed surface states are the topological equivalent to a single 2D Dirac cone at the surface Brillouin zone.

5.
J Phys Condens Matter ; 26(21): 212201, 2014 May 28.
Article in English | MEDLINE | ID: mdl-24804822

ABSTRACT

Resistivity, dc magnetization, and heat capacity measurements are reported for superconducting NaBi. T(c), the electronic contribution to the specific heat γ, the ΔC(p)/γT(c) ratio, and the Debye temperature are found to be 2.15 K, 3.4 mJ mol(-1) K(-2), 0.78, and 140 K respectively. The calculated electron-phonon coupling constant (λ(ep) = 0.62) implies that NaBi is a moderately coupled superconductor. The upper critical field and coherence length are found to be 250 Oe and 115 nm, respectively. Electronic structure calculations show NaBi to be a good metal, in agreement with the experiments; the p(x) and p(y) orbitals of Bi dominate the electronic states at the Fermi Energy.


Subject(s)
Bismuth/chemistry , Electric Conductivity , Electrons , Magnetics , Sodium/chemistry , Hot Temperature , Models, Chemical , Phonons , Temperature
6.
J Phys Condens Matter ; 26(20): 206002, 2014 May 21.
Article in English | MEDLINE | ID: mdl-24786568

ABSTRACT

We report that Sb2Te, a natural superlattice phase consisting of two elemental Sb2 layers interleaved with single Sb2Te3 layers, becomes ferromagnetic at low temperatures on doping with small percentages of Mn. Ferromagnetism appears for Mn concentrations as low as Sb1.98Mn0.02Te, where a ferromagnetic Tc of ~8.6 K is observed. Tc decreases with increasing Mn content in the stoichiometric materials but increases with increasing Te excess in materials of the type Sb1.93-yMn0.07Te1+y, starting at ~3 K at y = 0 and reaching a Tc of ~8.9 K at y = 0.06.


Subject(s)
Antimony/chemistry , Magnetic Fields , Magnets , Manganese/chemistry , Models, Chemical , Tellurium/chemistry , Computer Simulation , Electric Impedance
7.
Nat Commun ; 3: 1192, 2012.
Article in English | MEDLINE | ID: mdl-23149737

ABSTRACT

A topological insulator protected by time-reversal symmetry is realized via spin-orbit interaction-driven band inversion. The topological phase in the Bi(1-x)Sb(x) system is due to an odd number of band inversions. A related spin-orbit system, the Pb(1-x)Sn(x)Te, has long been known to contain an even number of inversions based on band theory. Here we experimentally investigate the possibility of a mirror symmetry-protected topological crystalline insulator phase in the Pb(1-x)Sn(x)Te class of materials that has been theoretically predicted to exist in its end compound SnTe. Our experimental results show that at a finite Pb composition above the topological inversion phase transition, the surface exhibits even number of spin-polarized Dirac cone states revealing mirror-protected topological order distinct from that observed in Bi(1-x)Sb(x). Our observation of the spin-polarized Dirac surface states in the inverted Pb(1-x)Sn(x)Te and their absence in the non-inverted compounds related via a topological phase transition provide the experimental groundwork for opening the research on novel topological order in quantum devices.

8.
Clin Pharmacol Ther ; 90(4): 568-74, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21881565

ABSTRACT

A common functional variant in paraoxonase 1 (PON1), Q192R, was recently reported to be a major determinant of clopidogrel response. This variant was genotyped in 566 participants of the Amish Pharmacogenomics of Anti-Platelet Intervention (PAPI) study and in 227 percutaneous coronary intervention (PCI) patients. Serum paraoxonase activity was measured in a subset of 79 PAPI participants. PON1 Q192R was not associated with pre- or post-clopidogrel platelet aggregation in the PAPI study (P = 0.16 and P = 0.21, respectively) or the PCI cohort (P = 0.47 and P = 0.91, respectively). The Q192 allele was not associated with cardiovascular events (hazard ratio (HR) 0.46, 95% confidence interval (CI) 0.20-1.06; P = 0.07). No correlation was observed between paraoxonase activity and post-clopidogrel platelet aggregation (r(2) < 0.01, P = 0.78). None of 49 additional PON1 variants evaluated was associated with post-clopidogrel platelet aggregation. These findings do not support a role for PON1 as a determinant of clopidogrel response.


Subject(s)
Aryldialkylphosphatase/genetics , Cardiovascular Diseases/genetics , Cardiovascular Diseases/mortality , Genetic Association Studies/methods , Genetic Variation/genetics , Ticlopidine/analogs & derivatives , Adult , Aged , Cardiovascular Diseases/drug therapy , Clopidogrel , Cohort Studies , Female , Follow-Up Studies , Humans , Male , Middle Aged , Survival Rate/trends , Ticlopidine/therapeutic use , Treatment Outcome
9.
Biochemistry ; 40(49): 14795-805, 2001 Dec 11.
Article in English | MEDLINE | ID: mdl-11732898

ABSTRACT

Cooperative ligand binding in the dimeric hemoglobin from the blood clam Scapharca inaequivalvis results primarily from tertiary, rather than quaternary, structural changes. Ligand binding is coupled with conformational changes of key residues, including Phe 97, which is extruded from the proximal heme pocket, and the heme group, which moves deeper into the heme pocket. We have tested the role of the heme movement in cooperative function by mutating Ile 114, at the base of the heme pocket. Replacement of this residue with a Met did not disturb the hemoglobin structure or significantly alter equilibrium ligand binding properties. In contrast, substitution with a Phe at position 114 inhibits the ligand-linked movement of the heme group, and substantially reduces oxygen affinity and cooperativity. As the extent of heme movement to the normal position of the ligated state is diminished, Phe 97 is inhibited from its movement into the interface upon ligand binding. These results indicate a tight coupling between these two key cooperative transitions and suggest that the heme movement may be an obligatory trigger for expulsion of Phe 97 from the heme pocket.


Subject(s)
Bivalvia/chemistry , Heme/chemistry , Hemoglobins/chemistry , Hemoglobins/metabolism , Protein Structure, Tertiary , Amino Acid Sequence , Animals , Bivalvia/metabolism , Hemoglobins/genetics , Ligands , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Protein Binding , Sequence Alignment
10.
EMBO Rep ; 2(8): 674-9, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11493595

ABSTRACT

Small globular proteins contain internal cavities and packing defects that reduce thermodynamic stability but seem to play a role in controlling function by defining pathways for the diffusion of the ligand/substrate to the active site. In the case of myoglobin (Mb), a prototype for structure-function relationship studies, the photosensitivity of the adduct of the reduced protein with CO, O2 and NO allows events related to the migration of the ligand through the matrix to be followed. The crystal structures of intermediate states of wild-type (wt) and mutant Mbs show the photolysed CO to be located either in the distal heme pocket (primary docking site) or in one of two alternative cavities (secondary docking sites) corresponding to packing defects accessible to an atom of xenon. These results convey the general picture that pre-existing internal cavities are involved in controlling the dynamics and reactivity of the reactions of Mb with O2 and other ligands, including NO.


Subject(s)
Myoglobin/chemistry , Protein Structure, Tertiary , Animals , Binding Sites , Carbon Monoxide/metabolism , Crystallography, X-Ray , Ligands , Models, Molecular , Myoglobin/metabolism , Oxygen/metabolism , Protein Binding , Xenon/metabolism
11.
J Biol Chem ; 276(7): 5177-88, 2001 Feb 16.
Article in English | MEDLINE | ID: mdl-11018046

ABSTRACT

The effects of mutagenesis on geminate and bimolecular O2 rebinding to 90 mutants at 27 different positions were used to map pathways for ligand movement into and out of sperm whale myoglobin. By analogy to a baseball glove, the protein "catches" and then "holds" incoming ligand molecules long enough to allow bond formation with the iron atom. Opening of the glove occurs by outward movements of the distal histidine (His(64)), and the ligands are trapped in the interior "webbing" of the distal pocket, in the space surrounded by Ile(28), Leu(29), Leu(32), Val(68), and Ile(107). The size of this pocket is a major determinant of the rate of ligand entry into the protein. Immediately after photo- or thermal dissociation, O2 moves away from the iron into this interior pocket. The majority of the dissociated ligands return to the active site and either rebind to the iron atom or escape through the His(64) gate. A fraction of the ligands migrate further away from the heme group into cavities that have been defined as Xe binding sites 4 and 1; however, most of these ligands also return to the distal pocket, and net escape through the interior of wild-type myoglobin is <20-25%.


Subject(s)
Myoglobin/chemistry , Myoglobin/metabolism , Oxygen/metabolism , Animals , Binding Sites , Heme/chemistry , Histidine/chemistry , Kinetics , Models, Chemical , Models, Molecular , Mutagenesis, Site-Directed , Myoglobin/genetics , Protein Binding , Water/chemistry , Whales
13.
Biochemistry ; 38(16): 5191-9, 1999 Apr 20.
Article in English | MEDLINE | ID: mdl-10213626

ABSTRACT

The two-state model [Monod, J., Wyman, J., and Changeux, J. P. (1965) J. Mol. Biol. 12, 88-118] postulates a single conformational change which, in the case of hemoglobin, has been related to the structural differences between deoxy and ligated hemoglobins [Perutz, M. F. (1979) Nature (London) 228, 726-739]. In its simplest form, the model does not represent satisfactorily either the equilibrium or the kinetics of the hemoglobin-oxygen reaction. The kinetic difficulty is with the rate of dissociation from the T-state, and may be met by assuming a wide difference in behavior between alpha- and beta-subunits. Experiments with Ni-Fe hybrids, however, show almost identical rates of combination with, and dissociation from, the two types of subunit, both of which develop R-like reactions as the pH is raised, the alpha-Fe-subunits at lower pH than the beta-Fe-subunits [Shibayama, N., Yonetani, T., Regan, R. M., and Gibson, Q. H. (1995) Biochemistry 34, 14658-14667]. The reactions of oxygen with hemoglobin A and the effect of pH upon them may be represented by assuming behavior of its subunits similar to that of the Ni-Fe hybrids. In such a scheme, alpha-alpha and beta-beta interactions become important elements in cooperativity, and more than two allosteric states are required, for reconsideration of the structural basis of cooperativity.


Subject(s)
Hemoglobin A/chemistry , Hemoglobin A/metabolism , Oxygen/chemistry , Oxygen/metabolism , Binding Sites , Buffers , Humans , Hydrogen-Ion Concentration , Iron/chemistry , Iron/metabolism , Kinetics , Ligands , Models, Chemical , Nickel/chemistry , Nickel/metabolism , Phosphates , Photolysis
14.
Biochemistry ; 38(7): 2117-26, 1999 Feb 16.
Article in English | MEDLINE | ID: mdl-10026295

ABSTRACT

The glbN gene of the cyanobacterium Nostoc commune UTEX 584 encodes a hemoprotein, named cyanoglobin, that has high oxygen affinity. The basis for the high oxygen affinity of cyanoglobin was investigated through kinetic studies that utilized stopped-flow spectrophotometry and flash photolysis. Association and dissociation rate constants were measured at 20 degrees C for oxygen, carbon monoxide, nitric oxide, and methyl and ethyl isocyanides. The association rate constants for the binding of these five ligands to cyanoglobin are the highest reported for any naturally occurring hemoglobin, suggesting an unhindered and apolar ligand binding pocket. Cyanoglobin also shows high rates of autoxidation and hemin loss, indicating that the prosthetic group is readily accessible to solvent. The ligand binding behavior of cyanoglobin was more similar to that of leghemoglobin a than to that of sperm whale myoglobin. Collectively, the data support the model of cyanoglobin function described by Hill et al. [(1996) J. Bacteriol. 178, 6587-6598], in which cyanoglobin sequesters oxygen, and presents it to, or is a part of, a terminal cytochrome oxidase complex in Nostoc commune UTEX 584 under microaerobic conditions, when nitrogen fixation, and thus ATP demand, is maximal.


Subject(s)
Bacterial Proteins , Cyanobacteria/chemistry , Hemoglobins/chemistry , Hemoglobins/metabolism , Carbon Monoxide/metabolism , Heme/chemistry , Hemin/metabolism , Hemoglobins/isolation & purification , Kinetics , Ligands , Methemoglobin/chemistry , Nitric Oxide/metabolism , Oxidation-Reduction , Oxygen/metabolism , Oxyhemoglobins/chemistry , Protein Binding , Truncated Hemoglobins
15.
Biophys J ; 76(3): 1259-69, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10049310

ABSTRACT

A triple mutant of sperm whale myoglobin (Mb) [Leu(B10) --> Tyr, His(E7) --> Gln, and Thr(E10) --> Arg, called Mb-YQR], investigated by stopped-flow, laser photolysis, crystallography, and molecular dynamics (MD) simulations, proved to be quite unusual. Rebinding of photodissociated NO, O2, and CO from within the protein (in a "geminate" mode) allows us to reach general conclusions about dynamics and cavities in proteins. The 3D structure of oxy Mb-YQR shows that bound O2 makes two H-bonds with Tyr(B10)29 and Gln(E7)64; on deoxygenation, these two residues move toward the space occupied by O2. The bimolecular rate constant for NO binding is the same as for wild-type, but those for CO and O2 binding are reduced 10-fold. While there is no geminate recombination with O2 and CO, geminate rebinding of NO displays an unusually large and very slow component, which is pretty much abolished in the presence of xenon. These results and MD simulations suggest that the ligand migrates in the protein matrix to a major "secondary site," located beneath Tyr(B10)29 and accessible via the motion of Ile(G8)107; this site is different from the "primary site" identified by others who investigated the photolyzed state of wild-type Mb by crystallography. Our hypothesis may rationalize the O2 binding properties of Mb-YQR, and more generally to propose a mechanism of control of ligand binding and dissociation in hemeproteins based on the dynamics of side chains that may (or may not) allow access to and direct temporary sequestration of the dissociated ligand in a docking site within the protein. This interpretation suggests that very fast (picosecond) fluctuations of amino acid side chains may play a crucial role in controlling O2 delivery to tissue at a rate compatible with physiology.


Subject(s)
Mutation , Myoglobin/chemistry , Myoglobin/genetics , Animals , Binding Sites , Biophysical Phenomena , Biophysics , Carbon Monoxide/metabolism , Crystallography, X-Ray , Diffusion , In Vitro Techniques , Kinetics , Ligands , Models, Molecular , Myoglobin/metabolism , Nitric Oxide/metabolism , Oxygen/metabolism , Photolysis , Protein Conformation , Thermodynamics , Whales
16.
Biochemistry ; 37(45): 15896-907, 1998 Nov 10.
Article in English | MEDLINE | ID: mdl-9843395

ABSTRACT

The isopropyl side chain of valine68 in myoglobin has been replaced by the acetamide side chain of asparagine in an attempt to engineer higher oxygen affinity. The asparagine replacement introduces a second hydrogen bond donor group into the distal heme pocket which could further stabilize bound oxygen. The Val68 to Asn substitution leads to approximately 3-fold increases in oxygen affinity and 4-6-fold decreases in CO affinity. As a result, the M-value (KCO/KO2) is lowered 15-20-fold to a value close to unity. An even larger enhancement of O2 affinity is seen when asparagine68 is inserted into H64L sperm whale myoglobin which lacks a distal histidine. The overall rate constants for oxygen and carbon monoxide binding to the single V68N myoglobin mutants are uniformly lower than those for the wild-type protein. In contrast, the overall rate constant for NO association is unchanged. Analyses of time courses monitoring the geminate recombination of ligands following nanosecond and picosecond flash photolysis of MbNO and MbO2 indicate that the barrier to ligand binding from within the heme pocket has been raised with little effect on the barrier to diffusion of the ligand into the pocket from the solvent. The crystal structures of the aquomet, deoxy, oxy, and carbon monoxy forms of the V68N mutant have been determined to resolutions ranging from 1.75 to 2.2 A at 150 K. The overall structures are very similar to those of the wild-type protein with the principal alterations taking place within and around the distal heme pocket. In all four structures the asparagine68 side chain lies almost parallel to the plane of the heme with its amide group directed toward the back of the distal heme pocket. The coordinated water molecule in the aquomet form and the bound oxygen in the oxy form can form hydrogen-bonding interactions with both the Asn68 amide group and the imidazole side chain of His64. Surprisingly, in the carbon monoxy form of the V68N mutant, the histidine64 side chain has swung completely out the distal pocket, its place being taken by two ordered water molecules. Overall, these functional and structural results show that the asparagine68 side chain (i) forms a strong hydrogen bond with bound oxygen through its -NH2 group but (ii) sterically hinders the approach of ligands to the iron from within the distal heme pocket.


Subject(s)
Amino Acid Substitution/genetics , Asparagine/genetics , Myoglobin/genetics , Myoglobin/metabolism , Oxygen/metabolism , Valine/genetics , Animals , Asparagine/metabolism , Binding Sites/genetics , Carbon Monoxide/chemistry , Carbon Monoxide/metabolism , Crystallization , Crystallography, X-Ray , Mutagenesis, Insertional , Mutagenesis, Site-Directed , Myoglobin/analogs & derivatives , Myoglobin/chemistry , Nitric Oxide/metabolism , Swine , Valine/metabolism , Whales
17.
Biophys J ; 75(2): 990-8, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9675199

ABSTRACT

Ligand binding studies were made with hemoglobin (Hb) isolated from trematode species Gastrothylax crumenifer (Gc), Paramphistomum epiclitum (Pe), Explanatum explanatum (Ee), parasitic worms of water buffalo Bubalus bubalis, and Isoparorchis hypselobagri (Ih) parasitic in the catfish Wallago attu. The kinetics of oxygen and carbon monoxide binding show very fast association rates. Whereas oxygen can be displaced on a millisecond time scale from human Hb at 25 degrees C, the dissociation of oxygen from trematode Hb may require a few seconds to over 20 s (for Hb Pe). Carbon monoxide dissociation is faster, however, than for other monomeric hemoglobins or myoglobins. Trematode hemoglobins also show a reduced rate of autoxidation; the oxy form is not readily oxidized by potassium ferricyanide, indicating that only the deoxy form reacts rapidly with this oxidizing agent. Unlike most vertebrate Hbs, the trematodes have a tyrosine residue at position E7 instead of the usual distal histidine. As for Hb Ascaris, which also displays a high oxygen affinity, the trematodes have a tyrosine in position B10; two H-bonds to the oxygen molecule are thought to be responsible for the very high oxygen affinity. The trematode hemoglobins display a combination of high association rates and very low dissociation rates, resulting in some of the highest oxygen affinities ever observed.


Subject(s)
Hemoglobins/metabolism , Oxyhemoglobins/metabolism , Trematoda/physiology , Animals , Aplysia , Ascaris , Buffaloes/parasitology , Carboxyhemoglobin/metabolism , Catfishes/parasitology , Humans , Kinetics , Myoglobin/metabolism , Oxidation-Reduction , Species Specificity
18.
Biochemistry ; 37(16): 5608-15, 1998 Apr 21.
Article in English | MEDLINE | ID: mdl-9548946

ABSTRACT

The effect of the apolar mutation of the distal histidine (His69-->Val) has been studied in the cooperative homodimeric hemoglobin from the mollusc Scapharca inaequivalvis. Absorption, circular dichroism, and resonance Raman spectroscopy point to a more symmetric heme structure of the deoxy derivative, which is indicative of an R-like conformation of the deoxy heme. Resonance Raman spectroscopy also brings out alterations in the geometry and interactions of the bound CO molecule. The iron-carbon stretching frequency is decreased by about 30 cm-1 with respect to the native protein, while the diatomic ligand stretching frequency is increased by about the same degree. Consistent with the structural changes, the ligand binding properties are significantly altered. In the mutant the overall rate and the affinity for CO binding are increased about 100-fold with respect to the native protein, and cooperativity is abolished. In addition, the amplitude and the rate of the geminate rebinding process increase significantly. This finding may be correlated to the longer average residence time of the photolyzed CO molecule within the heme pocket of the H69V mutant, as indicated by molecular dynamics simulations.


Subject(s)
Amino Acid Substitution/genetics , Hemoglobins/chemistry , Histidine/genetics , Mutagenesis, Insertional , Protein Conformation , Valine/genetics , Animals , Bivalvia , Carbon Monoxide/chemistry , Circular Dichroism , Dimerization , Heme/chemistry , Hemoglobins/genetics , Ligands , Protein Binding/genetics , Spectrophotometry , Spectrum Analysis, Raman
19.
Biochemistry ; 36(39): 11909-17, 1997 Sep 30.
Article in English | MEDLINE | ID: mdl-9305984

ABSTRACT

Geminate oxygen rebinding to myoglobin was followed from a few nanoseconds to a few microseconds after photolysis for more than 25 different oxymyoglobin point mutants in the presence and absence of 12 atm of xenon. In all cases, two relaxations were observed: an initial fast phase (half-time 20 ns) and a slower, smaller phase (half-time 0.5-2 micros). Generally, xenon accelerates the fast reaction but slows the slower reaction and diminishes its amplitude. The rates and proportions of the two components and the effects of xenon on them vary widely for different mutants. The locations of specific xenon binding sites [Tilton, R. F., Kuntz, I. D. Jr., and Petsko, G. A. (1984) Biochemistry 23, 2849-2857], the effects of point mutations on the geminate reactions, and molecular dynamics simulations were used to suggest locations in the protein interior occupied by ligands on the nanosecond to microsecond time scale. Photodissociated ligands may occupy xenon site 4 in the distal pocket and xenon site 1 below the plane of the heme. Rebinding from these positions corresponds to the slower geminate phase for O2 rebinding. The rapid geminate component is determined by competition between rebinding from a position closer to the iron atom and escape to solvent or more distant locations in the protein.


Subject(s)
Myoglobin/chemistry , Animals , Binding Sites , Ligands , Models, Chemical , Mutagenesis, Site-Directed , Myoglobin/genetics , Myoglobin/metabolism , Oxygen/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Whales , Xenon
20.
J Biol Chem ; 272(20): 13171-9, 1997 May 16.
Article in English | MEDLINE | ID: mdl-9148933

ABSTRACT

Residue Phe97, which is thought to play a central role in the cooperative functioning of Scapharca dimeric hemoglobin, has been mutated to leucine to test its proposed role in mediating cooperative oxygen binding. This results in an 8-fold increase in oxygen affinity and a marked decrease in cooperativity. Kinetic measurements of ligand binding to the Leu97 mutant suggest an altered unliganded (deoxy) state, which has been confirmed by high resolution crystal structures in the unliganded and carbon monoxide-liganded states. Analysis of the structures at allosteric end points reveals them to be remarkably similar to the corresponding wild-type structures, with differences confined to the disposition of residue 97 side chain, F-helix geometry, and the interface water structure. Increased oxygen affinity results from the absence of the Phe97 side chain, whose tight packing in the heme pocket of the deoxy state normally restricts the heme from assuming a high affinity conformation. The absence of the Phe97 side chain is also associated with diminished cooperativity, since Leu97 packs in the heme pocket in both states. Residual cooperativity appears to be coupled with observed structural transitions and suggests that parallel pathways for communication exist in Scapharca dimeric hemoglobin.


Subject(s)
Hemoglobins/chemistry , Allosteric Regulation/genetics , Animals , Bivalvia , Escherichia coli , Hemoglobins/genetics , Hemoglobins/metabolism , Molecular Sequence Data , Oxygen/metabolism , Phenylalanine/genetics , Point Mutation , Protein Folding
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