[Characteristics of the genome of poliovirus type 3 isolated from patients with poliomyelitis in Moldavia]. / Kharakteristika genoma shtammov poliovirusa tipa 3, vydelennykh v Moldavii ot bol'nykh poliomielitom.

Twelve poliovirus isolates of serotype 3 from patients with paralytic poliomyelitis have been analyzed by oligonucleotide mapping of the viral genomes. All the studied strains were isolated from patients in different regions of the Moldavian SSR in 1982. The maps of all isolates are similar but they do not practically possess any large oligonucleotides characteristic of the vaccine strain of type 3 poliovirus. It is concluded that a wild neurovirulent strain of type 3 poliovirus, that circulated in 1982 in the Moldavian SSR was the cause of paralytic poliomyelitis cases. All the studied isolates are suggested to have been derived relatively recently from the common ancestor.

[Characteristics of poliomyelitis viruses isolated from sewage]. / Izuchenie kharaketritiki virusov poliomielita, vydelennykh iz stochnykh vod.

Studies of the genetic markers of 39 (57.4%) strains of poliomyelitis virus type I isolated from sewage showed 7 of them to have genetic markers ret+40, Gu+, A+, Ag+ (Mahoney, Koñkov), 3 to be ret+40, Gu+, A+, Ag+ (Koñkov, Sabin), and 10 to be ret+40, Gu-, A-, Ag- (LSc 2ab). Among 20 (29.4%) strains of poliovirus type II two were ret+40, Gu+, A+, N+, Ag+ (MEF-1), and 2 were ret-40, Gu-, A-, N-, Ag- (P712 Ch2ab), and among 9 (15.2%) strains of type III only one (Saukett) had the genetic markers ret+40, Gu+, A+, Ag+. In the other 43 poliovirus strains under study the genetic markers did not correlate with each other. The possibility of using counter immunoelectrophoresis alongside with gel precipitation test for the study of antigenic intratype differences of polioviruses was demonstrated.