Cardiomyopathy in the mouse model of Duchenne muscular dystrophy caused by disordered secretion of vascular endothelial growth factor.

BACKGROUND: Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder that affects skeletal muscles and cardiac muscle tissue. In some cases, myocardial injury secondary to hypoxia can lead to dilative cardiomyopathy (DCM). A genetic defect in the dystrophin gene may increase the susceptibility of myocardium to hypoxia. Available data suggest that this may be caused by impaired secretion of NO, which is bound with secretion of VEGF-A. MATERIAL/METHODS: Male mice C57BI/10ScSn mdx (animal model of DMD) and healthy mice C57BI/10ScSn were exposed to hypobaric hypoxia in low-pressure chambers. Their hearts were harvested immediately after and 1, 3, 7, and 21 days after exposure to hypoxia. Normobaric mice were used as controls. The expression of VEGF-A in myocardium and cardiac vessel walls was evaluated using immunohistochemistry, Western blotting, and in situ hybridization. RESULTS: VEGF-A expression in myocardium and vessel walls of healthy mice peaked 24 hours after exposure to hypoxia. The expression of VEGF-A in vessel walls was similar in dystrophic and healthy mice; however, VEGF-A expression in the myocardium of dystrophic mice was impaired, peaking around day 7. In the heart, the total level of VEGF depends on VEGF expression in myocardium, not in vessel endothelium, and our research demonstrates that the expression of VEGF is dystrophin-dependent. CONCLUSIONS: Disordered secretion of VEGF-A in hypoxic myocardium caused the total level of this factor to be impaired in the heart. This factor, which in normal situations protect against hypoxia, promotes the gradual progression of cardiomyopathy.

Analysis of the upper massif of the craniofacial with the radial method - practical use.

INTRODUCTION: The analysis of the upper massif of the craniofacial (UMC) is widely used in many fields of science. The aim of the study was to create a high resolution computer system based on a digital information record and on vector graphics, that could enable dimension measuring and evaluation of craniofacial shape using the radial method. MATERIAL AND METHODS: The study was carried out on 184 skulls, in a good state of preservation, from the early middle ages. The examined skulls were fixed into Molisson's craniostat in the author's own modification. They were directed in space towards the Frankfurt plane and photographed in frontal norm with a digital camera. The parameters describing the plane and dimensional structure of the UMC and orbits were obtained thanks to the computer analysis of the function recordings picturing the craniofacial structures and using software combining raster graphics with vector graphics. RESULTS: It was compared mean values of both orbits separately for male and female groups. In female skulls the comparison of the left and right side did not show statistically significant differences. In male group, higher values were observed for the right side. Only the circularity index presented higher values for the left side. CONCLUSIONS: Computer graphics with the software used for analysing digital pictures of UMC and orbits increase the precision of measurements as well as the calculation possibilities. Recognition of the face in the post mortem examination is crucial for those working on identification in anthropology and criminology laboratories.

The description of vascular variations in three-dimensional space: a novel method of spatial cerebral arteries evaluation.

BACKGROUND: Anatomical and clinical descriptions of variations in arteries are based solely on anatomical terminology. New diagnostic techniques depend on terms such as the anterior, posterior, superior, inferior, medial, and lateral location of arteries and their branches or bifurcations, trifurcations, and fenestrations. However, these methods may be obsolete and such a simplistic approach is inadequate, especially for procedures that require precise morphological and morphometrical descriptions in 3D space. This is critical for neruonavigational surgery and interventional vascular radiology. MATERIAL/METHODS: This paper presents a new system for the accurate location and description of structural variations in vessels which is based on the alpha, beta, and gamma direction cosines. The algorithm can be summarized in the following steps: acquisition of the point coordinates in 3D space, interpolation, recovery of the 3D point coordinates, and calculation of the interpolated trajectory. RESULTS: New parameters (cos alpha, beta, and gamma, the tortuous index TI, and the deviation index DI) were calculated for the spatial description of vessels. CONCLUSIONS: By using this new method, new vascular descriptive methods have been proposed. It is possible that new categories as well as vessel variations may be found. The curvature and tortuosity of the vascular segments can also be considered more effective when incorporated into the description of vessels, especially in cases where pathologies such as tumors have altered the vessel in some way.

[Transplantation of islets of langerhans--alternative or solution in type 1 diabetes treatment?]. / Przeszczep wysp trzustkowych langerhansa--alternatywa czy rozwiazanie w leczeniu cukrzycy typu 1?

Diabetes mellitus is severe disease caused by partial or total lack of insulin leading to microangiopathy. Methods of treatment used today do not protect patients from the complications of disease. The proposed alternative of the treatment is transplantation of the pancreas islets. Till June 2003, 705 transplantation were performed worldwide. Allotransplant trials were described so far, while xenotransplants may appear as an alternative using alien species donors. Immune incompatibility of a human and animals is a major problem in the method, which can be solved by the use of genetically modified animals. In vitro genetic modifications of the pancreatic islets were already undertaken. New perspective arose with the development of the "stem cells" technology--differentiation of the primary cells into the Langerhans islets cells. Contemporary data show positive value of the method and give new perspective in the treatment of diabetes mellitus with respect to its rising morbidity.

Is manual foot lenght measurement of comparable value to ultrasound femur and humerus measurement in anatomical studies for the assessment of foetal age?

Contemporary anatomical studies require reliable methods for determining foetal age. Menstrual age is often found to be inadequate. A combination of several anatomical features showing age-dependency may result both in exact age approximation and pathology detection. The authors compared the manual foot length measurements with the ultrasound femur and humerus length measurements of aborted foetuses in the calculation of foetal age. The correlation between femur length and foot length as well as humerus length and foot length were statistically significant. The expected value formulae for foot length are presented. The authors conclude that foetal age assessment based on foot length metering is reliable before the 7th calendar month of pregnancy and correlates with ultrasound measurements of the humerus and femur.

Digital analysis of the dynamics of the arterial supply to the human foetal kidneys.

Variations in the renal arteries in human individuals and foetuses have already been well studied. Contemporary trends in visualisation techniques focus on the evaluation of the dynamic parameters of blood flow in the vessels (speed, pulsatility, resistance). Most of these data have been obtained by the means of Doppler ultrasound (Fig. 1, 2). The authors have not found any anatomical database containing information about variability in the volume of the foetal renal arteries. The aim of the study is to design a database for variation in foetal renal artery volume in relation to foetal age and sex. The material consisted of digital images of the renal arteries filled with LBS-latex taken from 30 foetuses aged 12-19 Hbd. Digital analysis of the arteries was made with a unique form of software. The program is a 2D vector graphic editor using spliced functions of Bezier. Foetal age is estimated according to the last menstrual period and measurement of manual foot length and femur length (FL) as determined by ultrasound.

Digital analysis of the volume of the human foetal suprarenal arteries.

Vascularisation of an organ is an index of its metabolic activity. The suprarenal glands are of crucial importance in the development of pregnancy. No data were found by the authors to describe the volume of the human foetal suprarenal arteries throughout pregnancy. The study was designed to form a database of human foetal suprarenal arterial volume in relation to foetal age and sex. Digital images were obtained at 4-week intervals of the suprarenal arteries of 30 foetuses aged between 12-40 Hbd. The arteries were primarily filled with LBS latex. A unique form of software was designed to assist in incorporating vector graphics, spliced functions of Bezier, into the analysis. The arteries contoured by the geometric curves were calculated for their initial, average and terminal diameter, length and volume. The measurements were compared in relation to foetal age and sex at 4-week intervals. Foetal age was assessed by means of calculation from the last menstrual period, manual measurement of foot length and ultrasonagraphic measurement of femoral length. The suprarenal arteries in human foetuses are of strongly individual variation both in their origin and quantity. The volume of the arteries appears constant in the group analysed.

Growth dynamics of the renal and suprarenal arteries in human foetuses.

The kidneys and suprarenal cortex are of common embryonic origin. The suprarenal gland and kidney have a common pathway in angiogenesis. Each of the organs is of key importance for intrauterine and individual development, yet they vary greatly in growth dynamics throughout pregnancy. The authors compared the arterial supply of these organs quantitatively in respect to foetal age and sex.

Digital-image analysis of the femoral shaft/neck angle in human foetuses.

Measurements were made of the femoral shaft/neck angle (CCD angle) in 106 human foetuses, aged from 16 to 38 hbd, using a "FEM-GEO_03" computer program. The values of the CCD angle in the group under examination were: mean = 140.48 degrees, SD = 6.95 degrees, max = 157.90 degrees, min = 113.93 degrees. No significant differences were found in CCD angle size between male and female foetuses or between left and right bones. Dispersion analysis showed a decrease in the CCD angle during foetal development, which suggests that adaptation to a vertical position and bipedal gait starts during pregnancy and is manifest as an inborn feature.