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1.
Radiol Case Rep ; 15(4): 431-434, 2020 Apr.
Article in English | MEDLINE | ID: mdl-32089761

ABSTRACT

Myxoid liposarcoma is the second most common type of liposarcoma, normally located in deep tissues of the lower extremities and rarely in the mesenchyma of abdomen and pelvis We present a patient who, incidentally, showed a large pelvis mass. CT and MR revealed a loculated lesión with hypodense areas and very high signal in T2 respectively as well as heterogeneous contrast enhancement. The imaging findings of pelvic myxoid liposarcoma are nonspecific, but nevertheless a painless mesenchymal mass should be considered when we see lesions of myxoid aspect in the pelvic area without a clear relationship with defined anatomic structures.

2.
Sci Rep ; 7: 46755, 2017 05 02.
Article in English | MEDLINE | ID: mdl-28462930

ABSTRACT

Viral nervous necrosis (VNN) certainly represents the biggest challenge for the sustainability and the development of aquaculture. A large number of economically relevant fish species have proven to be susceptible to the disease. Conversely, gilthead sea bream has generally been considered resistant to VNN, although it has been possible to isolate the virus from apparently healthy sea bream and sporadically from affected larvae and postlarvae. Unexpectedly, in 2014-2016 an increasing number of hatcheries in Europe have experienced mass mortalities in sea bream larvae. Two clinical outbreaks were monitored over this time span and findings are reported in this paper. Despite showing no specific clinical signs, the affected fish displayed high mortality and histological lesions typical of VNN. Fish tested positive for betanodavirus by different laboratory techniques. The isolates were all genetically characterized as being reassortant strains RGNNV/SJNNV. A genetic characterization of all sea bream betanodaviruses which had been isolated in the past had revealed that the majority of the strains infecting sea bream are actually RGNNV/SJNNV. Taken together, this information strongly suggests that RGNNV/SJNNV betanodavirus possesses a particular tropism to sea bream, which can pose a new and unexpected threat to the Mediterranean aquaculture.


Subject(s)
Fish Diseases/virology , Nodaviridae/physiology , RNA Virus Infections/virology , Reassortant Viruses/physiology , Sea Bream/virology , Animals , Aquaculture , Female , Genotype , Host-Pathogen Interactions , Larva/virology , Male , Mediterranean Region , Nodaviridae/classification , Nodaviridae/genetics , Phylogeny , RNA, Viral/genetics , Reassortant Viruses/classification , Reassortant Viruses/genetics
3.
Int J Syst Evol Microbiol ; 62(Pt 2): 425-429, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21460137

ABSTRACT

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09(T), was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum 'Bacteroidetes'). Sequence similarities between the isolate and type strains of other members of the genus were 93.1-97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C(15 : 0) (24.8 %), iso-C(15 : 0) 3-OH (18.0 %), anteiso-C(15 : 0) (8.1 %), C(15 : 1)ω6c (6.9 %) and iso-C(15 : 1) (6.2 %). Genotypic and phenotypic data indicate that strain 35/09(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09(T) ( = CECT 7612(T) = NCIMB 14598(T)).


Subject(s)
Bass/microbiology , Fish Diseases/microbiology , Flavobacteriaceae Infections/veterinary , Tenacibaculum/classification , Tenacibaculum/isolation & purification , Animals , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Fatty Acids/analysis , Flavobacteriaceae Infections/microbiology , Genes, rRNA , Genotype , Molecular Sequence Data , Phenotype , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Spain , Tenacibaculum/chemistry , Tenacibaculum/genetics
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