Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 37
Filter
Add more filters










Publication year range
1.
Nanotechnology ; 33(5)2021 Nov 12.
Article in English | MEDLINE | ID: mdl-34678792

ABSTRACT

Nitrogen-doping of cadmium sulfide nanostructured compounds was carried out under a nitrogen plasma source to produce CdS-N compounds. Once prepared, it was supported on graphene oxide sheets for producing CdS-N/GO photocatalysts, which were tested in the degradation of lignin and methylene blue (MB) molecules. Photocatalytic reactions were carried out under UV and visible (vis) energy irradiation. To provide insight on the catalytic behavior the CdS, CdS-N, GO, and CdS-N/GO compounds were characterized using different techniques including x-ray diffraction, scanning electron microscopy, Raman, and UV-vis diffuse reflectance spectroscopy. X-ray photoelectron spectroscopy allowed determining the chemical composition in samples. It was observed an outstanding performance in photocatalytic activity tests, attributed to the extended response towards the visible light regime, and the synergistic effect between CdS-N and GO particles. The catalytic activity tests, reveal that the CdS-N/GO compound achieved over 90% lignin degradation and 100% of MB degradation. In addition, a remarkable performance is observed in the CdS-N/GO compound which exhibited stability after performing several reaction cycles.

2.
Nanotechnology ; 32(40)2021 Jul 14.
Article in English | MEDLINE | ID: mdl-34186516

ABSTRACT

The present study was aimed to develop nitrogen-doped nanostructured ZnO thin films. These films were produced in a sequential procedure involving the atomic layer deposition technique, and a hydrothermal process supported by microwave heating. Employing the atomic layer deposition technique, through self-limited reactions of diethylzinc (DEZn) and H2O, carried out at 3.29 × 10-4atm and 190 °C, a high-quality ZnO seed was grown on a Si (100) substrate, producing a textured film. In a second stage, columnar ZnO nanostructures were grown perpendicularly oriented to the silicon substrate on those films, using a solvothermal process in a microwave heating facility, employing Zn(NO3)2as zinc precursor, while hexamethylenetetramine (HMTA) was used to produce the bridging of Zn2+ions. The consequence of N-doping concentration on the physicochemical properties of ZnO thin films was studied. The manufactured films were structurally analyzed by scanning electron microscopy and x-ray diffraction. Also, x-ray photoelectron spectroscopy, Raman, and UV-vis spectroscopies were used to provide further insight on the effect of nitrogen doping. The N-doped films displayed textured wurtzite-like structures that changes their preferential growth from the (002) to the (100) crystallographic plane, apparently promoted by the increase of nitrogen precursor. It is also shown that nitrogen-doped films undergo a reduction in their bandgap, compared to ZnO. The methodology presented here provides a viable way to perform high-quality N-ZnO nanostructured thin films.

3.
Nanotechnology ; 32(14): 145601, 2021 Apr 02.
Article in English | MEDLINE | ID: mdl-33352537

ABSTRACT

Nanostructured ZnO nanoarrays deposited on silicon oriented substrates is a very promising area in the study of the control of physicochemical properties, in which photoluminescence plays a crucial role. This optical property inherent to ZnO, can be favorably modified through the inclusion of doping elements, with the purpose of appropriately modifying their optical absorption and luminescence. Following this objective, in the present work we present the development of Zn(1-x-y)Ce(x)Eu(y)O nanostructured thin films. The samples were produced in two steps process by atomic layer deposition technique followed by a solvothermal synthesis. The purpose of Cerium and Europium incorporation into the ZnO compound is to enhance the photoluminescence in ZnO thin films. In a first stage textured thin films were obtained from diethylzinc at a temperature of 190 °C and a pressure of 3.29 × 10-4 atm, on silicon substrates (111). Subsequently, the perpendicular growth of nanostructures was induced under a solvothermal process, where Zn(NO3)2 was used as Zn precursor and hexamethylene-tetramine operating as a dual-ligand to promote the linking of Zn2+ ions. The growth of cerium-europium ZnO nanostructures was promoted with Ce(C2H3O2)3·H2O and Eu(NO3)3·5H2O. The obtained Zn(1-x-y)Ce(x)Eu(y)O nanostructured thin films, were examined through SEM-microscopy, x-ray diffraction, x-ray photoelectron spectroscopy and photoluminescence studies. The attained results show that it is feasible to produce Ce-Eu-doped ZnO nanostructures with tailored photoluminescence and crystal size. Interestingly the Ce-Eu doping induces a strong shift in comparison to the typical UV emission of ZnO; an effect that can be related with the increase of lattice defects in ZnO.

4.
Mater Sci Eng C Mater Biol Appl ; 117: 111290, 2020 Dec.
Article in English | MEDLINE | ID: mdl-32919651

ABSTRACT

Nanocrystalline titania (TiO2) is one of the most investigated crystalline nanostructured systems in the field of materials science. The technological applications of this material are related to its optoelectronic and photocatalytic properties, which in turn are strongly dependent on the crystal phase (i.e., anatase, brookite, and rutile), particle size, and surface structure. However, systematic comparative studies of all its crystal phases are scarce in literature due to difficulties in providing a controlled synthesis, which is primarily important in obtaining the brookite phase. In this report, the synthesis of TiO2 nanoparticles in the anatase, brookite, and rutile structures was explored, using amorphous TiO2 as a common precursor under microwave-assisted hydrothermal conditions. The influence of parameters such as temperature, acidity, and precursor concentration on phase crystallization were investigated. The TiO2 materials (amorphous and crystalline phases as well as commercial Degussa P25) were systematically characterized using Raman spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, UV-visible reflectance spectroscopy, and dynamic and electrophoretic light scattering. The bactericidal activity and photocatalytic antibacterial effectiveness of each material were evaluated through the determination of the minimum inhibitory and bactericidal concentrations, and via the mortality kinetic method under ultraviolet (UV) illumination under similar conditions with two bacterial groups of unique cellular structures: Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). The results are discussed with particular emphasis on the relationship between the synthesis parameters (acidity, precursor concentration, temperature and reaction time) and the bactericidal properties.


Subject(s)
Microwaves , Staphylococcus aureus , Catalysis , Escherichia coli , Titanium
5.
Appl Opt ; 59(13): D201-D209, 2020 May 01.
Article in English | MEDLINE | ID: mdl-32400643

ABSTRACT

Mixed-halide organic-inorganic hybrid perovskites are considered promising light-absorbing materials in the development of solar cells related to the obtained high-power conversion efficiency. Current efforts are focused on the study of the energy-conversion mechanisms, where the nonradiative recombination pathway is the least explored. In this work, a combination of optical and photoacoustic spectroscopies is used to determine the visible spectral light-into-heat conversion efficiency of lead-based mixed-halide organic-inorganic hybrid perovskites in a semicomplete n-i-p mesoscopic perovskite solar cell (PSC). A remarkable average conversion efficiency of about 87% has been found for the nonradiative combination in the perovskite, with the estimated composition ${{\rm FA}_{0.71}}{{\rm MA}_{0.29}}{{\rm PbI}_{2.9}}{{\rm Br}_{0.1}}$FA0.71MA0.29PbI2.9Br0.1 in the wavelength range of 400 to 800 nm. As a result, 13% of the incident light is transformed in radiative recombination processes and/or photodegradation of the material. Furthermore, the extinction coefficient and refractive index of the material are reported, and it was found that the optical constants and the optical absorption in the short-wavelength range are significantly smaller than previously reported for${{\rm MAPbI}_3}$MAPbI3.

6.
Sci Rep ; 9(1): 14687, 2019 Oct 11.
Article in English | MEDLINE | ID: mdl-31604979

ABSTRACT

Hysteresis loops exhibited by the thermal properties of undoped and 0.8 at.% W-doped nanocrystalline powders of VO2 synthesized by means of the solution combustion method and compacted in pellets, are experimentally measured by photothermal radiometry. It is shown that: (i) the W doping reduces both the hysteresis loops of VO2 and its transition temperature up to 15 °C. (ii) The thermal diffusivity decreases (increases) until (after) the metallic domains become dominant in the VO2 insulating matrix, such that its variation across the metal-insulation transition is enhanced by 23.5% with W-0.8 at.% doping. By contrast, thermal conductivity (thermal effusivity) increases up to 45% (40%) as the metallic phase emerges in the VO2 structure due to the insulator-to-metal transition, and it enhances up to 11% (25%) in the insulator state when the local rutile phase is induced by the tungsten doping. (iii) The characteristic peak of the VO2 specific heat capacity is observed in both heating and cooling processes, such that the phase transition of the 0.8 at.% W-doped sample requires about 24% less thermal energy than the undoped one. (iv) The impact of the W doping on the four above-mentioned thermal properties of VO2 mainly shows up in its insulator phase, as a result of the distortion of the local lattice induced by the electrons of tungsten. W doping at 0.8 at.% thus enhances the VO2 capability to transport heat but diminishes its thermal switching efficiency.

7.
Chemosphere ; 236: 124368, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31323553

ABSTRACT

In the present work, the photocatalytic efficiency of a novel system based on ZnO doped with nitrogen (ZT) and supported on graphene oxide (GO) is investigated. ZnO synthesis and their N doping were carried out in a microwave reactor using thiourea as nitrogen source, while the GO was prepared through a variation of the Hummers' method. Structural, morphological and photochemical characterization of the developed material was performed by X-ray diffraction (XRD), UV-Vis spectroscopy, energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), analysis by X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy. The compounds were used to photodegrade the methylene blue molecule, which confirms the efficiency of nitrogen doped supported system compared to pristine ZnO. The degradation percentage of MB under UV energy using nitrogen-doped ZnO/GO, in a time of 35 min, reached 98% degradation; while using visible light 93% of degradation was reached.


Subject(s)
Graphite/chemistry , Nitrogen/chemistry , Zinc Oxide/chemistry , Catalysis , Photolysis
8.
Sci Rep ; 8(1): 8479, 2018 May 31.
Article in English | MEDLINE | ID: mdl-29855507

ABSTRACT

Hysteresis loops in the emissivity of VO2 thin films grown on sapphire and silicon substrates by a pulsed laser deposition process are experimentally measured through the thermal-wave resonant cavity technique. Remarkable variations of about 43% are observed in the emissivity of both VO2 films, within their insulator-to-metal and metal-to-insulator transitions. It is shown that: i) The principal hysteresis width (maximum slope) in the VO2 emissivity of the VO2 + silicon sample is around 3 times higher (lower) than the corresponding one of the VO2 + sapphire sample. VO2 synthesized on silicon thus exhibits a wider principal hysteresis loop with slower MIT than VO2 on sapphire, as a result of the significant differences on the VO2 film microstructures induced by the silicon or sapphire substrates. ii) The hysteresis width along with the rate of change of the VO2 emissivity in a VO2 + substrate sample can be tuned with its secondary hysteresis loop. iii) VO2 samples can be used to build a radiative thermal diode able to operate with a rectification factor as high as 87%, when the temperature difference of its two terminals is around 17 °C. This record-breaking rectification constitutes the highest one reported in literature, for a relatively small temperature change of diode terminals.

9.
J Pharm Biomed Anal ; 139: 238-246, 2017 May 30.
Article in English | MEDLINE | ID: mdl-28314215

ABSTRACT

Tobacco smoke exposure is the principal cause of lung tissue destruction, which in turn results in emphysema that leads into shortness of breath. Liver growth factor (LGF, a cell and tissue regenerating factor with therapeutic activity in several organs) has antifibrotic and antioxidant properties that could be useful to promote lung tissue regenerating capacity in damaged lungs. The current study has examined differences in metabolite profiles (fingerprints) of plasma from mice (strain C57BL/6J, susceptible to develop emphysema) exposed to tobacco smoke during six months. One group of mice received a treatment with Liver Growth Factor (LGF) after emphysema was established, whereas the other group did not receive the treatment. Age and sex-matched mice not exposed to smoke were also maintained with or without treatment as controls. Metabolic fingerprints (untargeted analysis) of plasma after protein precipitation were obtained by LC-QTOF-MS. The signals were processed and a large number of possible metabolites were found (23944). Multivariate data analysis provided models that highlighted the differences between control and smoke exposed mice in both conditions. Accurate masses of features (possible compounds) representing significant differences were searched using online public databases. Lipid mediators, related to intracellular signaling in inflammation, were found among the metabolites putatively identified as markers of the different conditions and among them, sphingosine, sphingosine 1-phosphate and lysophospholipids point at the relevance of such metabolites in the regulation of the processes related to tissue regeneration mediated by LGF. These results also suggest that metabolomic fingerprinting could potentially guide the characterization of relevant metabolites leading the regeneration of lungs in emphysema disease.


Subject(s)
Bilirubin/therapeutic use , Lysophospholipids/metabolism , Metabolomics/methods , Pulmonary Emphysema/metabolism , Serum Albumin/therapeutic use , Smoking/adverse effects , Sphingosine/analogs & derivatives , Animals , Bilirubin/pharmacology , Inhalation Exposure/adverse effects , Male , Mice , Mice, Inbred C57BL , Pulmonary Emphysema/drug therapy , Serum Albumin/pharmacology , Serum Albumin, Human , Smoking/drug therapy , Sphingosine/metabolism
10.
Allergy ; 71(8): 1095-102, 2016 08.
Article in English | MEDLINE | ID: mdl-27100940

ABSTRACT

The prick test is one of the most common medical methods for diagnosing allergies, and it has been carried out in a similar and laborious manner over many decades. In an attempt to standardize the reading of the test, many researchers have tried to automate the process of measuring the allergic reactions found by developing systems and algorithms based on multiple technologies. This work reviews the techniques for automatic wheal measurement with the aim of pointing out their advantages and disadvantages and the progress in the field. Furthermore, it provides a classification scheme for the different technologies applied. The works discussed herein provide evidence that significant challenges still exist for the development of an automatic wheal measurement system that not only helps allergists in their medical practice but also allows for the standardization of the reading and data exchange. As such, the aim of the work was to serve as guideline for the development of a proper and feasible system.


Subject(s)
Hypersensitivity/diagnosis , Skin Tests/methods , Skin Tests/standards , Allergens/classification , Allergens/immunology , Automation, Laboratory , Humans , Hypersensitivity/immunology , Skin Tests/trends
11.
Rev Sci Instrum ; 86(12): 123101, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26724000

ABSTRACT

We present a modification of the laser-induced transient grating setup enabling continuous tuning of the transient grating period. The fine control of the period is accomplished by varying the angle of the diffraction grating used to split excitation and probe beams. The setup has been tested by measuring dispersion of bulk and surface acoustic waves in both transmission and reflection geometries. The presented modification is fully compatible with optical heterodyne detection and can be easily implemented in any transient grating setup.

12.
Am J Physiol Endocrinol Metab ; 308(2): E111-21, 2015 Jan 15.
Article in English | MEDLINE | ID: mdl-25389365

ABSTRACT

The aim of the present work was to determine the effects of liver growth factor (LGF) on the regeneration process of rat testes after chemical castration induced by ethane dimethanesulfonate (EDS) by analyzing some of the most relevant proteins involved in cholesterol metabolism, such as hormone sensitive lipase (HSL), 3ß-hydroxysteroid dehydrogenase (3ß-HSD), scavenger receptor SR-BI, and other components of the SR family that could contribute to the recovery of steroidogenesis and spermatogenesis in the testis. Sixty male rats were randomized to nontreated (controls) and LGF-treated, EDS-treated, and EDS + LGF-treated groups. Testes were obtained on days 10 (T1), 21 (T2), and 35 (T3) after EDS treatment, embedded in paraffin, and analyzed by immunohistochemistry and Western blot. LGF improved the recovery of the seminiferous epithelia, the appearance of the mature pattern of Leydig cell interstitial distribution, and the expression of mature SR-BI. Moreover, LGF treatment resulted in partial recovery of HSL expression in Leydig cells and spermatogonia. No changes in serum testosterone were observed in control or LGF-treated rats, but in EDS-castrated animals LGF treatment induced a progressive increase in serum testosterone levels and 3ß-HSD expression. Based on the pivotal role of SR-BI in the uptake of cholesteryl esters from HDL, it is suggested that the observed effects of LGF would facilitate the provision of cholesterol for sperm cell growth and Leydig cell recovery.


Subject(s)
Bilirubin/pharmacology , CD36 Antigens/metabolism , Leydig Cells/metabolism , Serum Albumin/pharmacology , Spermatogenesis/physiology , Sterol Esterase/metabolism , Testis/metabolism , Animals , Blotting, Western , Immunohistochemistry , Male , Mesylates/administration & dosage , Random Allocation , Rats , Rats, Wistar , Serum Albumin, Human , Sperm Motility , Testis/cytology , Testosterone/blood
13.
Carbohydr Polym ; 99: 84-90, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24274482

ABSTRACT

Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields. In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields. Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration. The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM). The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity. Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.


Subject(s)
Agar/chemistry , Biocompatible Materials/chemical synthesis , Iron Compounds/chemistry , Rhodophyta/chemistry , Hot Temperature , Magnetic Fields , Microscopy, Electron, Scanning
14.
Rev Sci Instrum ; 84(2): 024903, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23464238

ABSTRACT

Thermal properties of solids are obtained by fitting the exact complex photothermal model to the normalized photoacoustic (PA) signal in the front configuration. Simple closed-form expressions for the amplitude and phase are presented in all frequency ranges. In photoacoustic it has been common practice to assume that all the absorptions of radiation take place in the sample. However, in order to obtain the accurate thermal properties it is necessary to consider the PA signal contributions produced at the cell walls. Such contributions were considered in our study. To demonstrate the usefulness of the proposed methodology, commercial stainless steel layers AISI 302 were analyzed. It is shown that using our approach the obtained thermal diffusivity and effusivity were in good agreement with those reported in the literature. Also, a detailed procedure for the calculation of the standard error in the thermal properties is discussed.

15.
J Struct Biol ; 177(2): 349-57, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22197647

ABSTRACT

Black corals (antipatharians) are colonial cnidarians whose branched tree-like skeleton is mostly constituted of chitin fibrils inside a lipoproteic matrix. The skeleton exhibits growth rings formed by chitin layers (micro-lamellae). In order to know the effect of the arrangement microlamellae of chitin of black corals and to improve the understanding of the role of chitin structure in the antipatharian skeleton, the mechanical properties of the skeleton of two black corals, Antipathes caribbeana and Antipathes pennacea, were examined using nanoindentation tests. Measurements of reduced elastic modulus, nanohardness and the viscoelastic behavior were measured with a spheroconical indenter. The results indicate variations in the values of the mechanical properties clearly associated with different structures present in the skeletons, the core being the one that invariably shows the maximum values. The solid multilamellar arrangement of black coral chitin, its viscoelastic behavior, and the anisotropic mechanical response, are relevant factors contributing to the successful adaptation of black coral colonies to shallow as well as to very deep waters.


Subject(s)
Anthozoa/anatomy & histology , Chitin/metabolism , Algorithms , Animals , Anthozoa/metabolism , Biomechanical Phenomena , Elastic Modulus , Microscopy, Atomic Force , Surface Properties
16.
Opt Express ; 17(15): 12794-806, 2009 Jul 20.
Article in English | MEDLINE | ID: mdl-19654685

ABSTRACT

The measurement of the Mueller matrix when the probing beam is placed on the boundary between two (or more) regions of the sample with different optical properties may lead to a depolarization in the Mueller matrix. The depolarization is due to the incoherent superposition of the optical responses of different sample regions in the probe beam. Despite of the depolarization, the measured Mueller matrix has information enough to subtract a Mueller matrix corresponding to one of the regions of sample provided that this subtracted matrix is non-depolarizing. For clarity, we will call these non-depolarizing Mueller matrices of one individual region of the sample simply as the non-depolarizing components. In the framework of the theory of Mueller matrix algebra, we have implemented a procedure allowing the retrieval of a non-depolarizing component from a depolarizing Mueller matrix constituted by the sum of several non-depolarizing components. In order to apply the procedure, the Mueller matrices of the rest of the non-depolarizing components have to be known. Here we present a numerical and algebraic approaches to implement the subtraction method. To illustrate our method as well as the performance of the two approaches, we present two practical examples. In both cases we have measured depolarizing Mueller matrices by positioning an illumination beam on the boundary between two and three different regions of a sample, respectively. The goal was to retrieve the non-depolarizing Mueller matrix of one of those regions from the measured depolarizing Mueller matrix. In order to evaluate the performance of the method we compared the subtracted matrix with the Mueller matrix of the selected region measured separately.


Subject(s)
Optics and Photonics , Algorithms , Image Processing, Computer-Assisted , Light , Microscopy, Polarization/methods , Models, Statistical , Physics/methods
18.
Sci Total Environ ; 334-335: 359-76, 2004 Dec 01.
Article in English | MEDLINE | ID: mdl-15504522

ABSTRACT

This paper summarises the results of a series of studies on the interpretation of time series of levels of total suspended particles (TSP) and particulate matter (PM, <10 microm) in six regions of Spain in the period 1996-2000. In addition to the local pollution events, high PM10 episodes are recorded during African dust outbreaks, regional atmospheric recirculation events (mainly in spring to autumn), and to a lesser extent, under the influence of European and Mediterranean long range transported air masses. The lowest PM10 levels are usually recorded under Atlantic air mass advective conditions. All these regional and large-scale processes account for the relatively high PM10 levels recorded in regional background stations in Spain. Thus, the PM10 levels recorded at EMEP (Cooperative Program for Monitoring and Evaluation of the Long Range Transmission of Air Pollutants in Europe) regional background stations between March 2001 and March 2002 are very close to the annual limit value proposed for 2010 by the EU Air Quality Directive 1999/30/CE. Chemical data obtained for the different monitoring stations during 2001 show a high mineral load in PM10 for most of the study sites in Spain. Furthermore, a high marine aerosol load is evidenced in the Canary Islands. These mineral and marine loads are lower when considering PM2.5, but a relatively high proportion (8-21%) of mineral dust is still present.

19.
J Opt Soc Am A Opt Image Sci Vis ; 17(2): 328-34, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10680635

ABSTRACT

A complete and minimum set of necessary and sufficient conditions for a real 4 x 4 matrix to be a physical Mueller matrix is obtained. An additional condition is presented to complete the set of known conditions, namely, the four conditions obtained from the nonnegativity of the eigenvalues of the Hermitian matrix H associated with a Mueller matrix M and the transmittance condition. Using the properties of H, a demonstration is also presented of Tr(M(T)M) = 4m(2)00 as being a necessary and sufficient condition for a physical Mueller matrix to be a pure Mueller matrix.


Subject(s)
Models, Theoretical , Optics and Photonics
20.
J Hepatol ; 30(6): 1065-72, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10406185

ABSTRACT

BACKGROUND/AIMS: Most substances used in experimental models of cirrhosis are chosen either as protectors of lipid peroxidation, as antifibrogenic agents or as vitamins, among others. In this report, we analyze the improvement produced, in established cirrhosis (CCl4 plus phenobarbital) in rats, by intraperitoneal injection of Liver Growth Factor, a hepatic mitogen with activity both in vivo and in vitro. METHODS: Following confirmation of CCl4-induced cirrhosis, Liver Growth Factor (4.5 microg per ratx2 injections/week for 3 weeks) was administered to one group of rats (Cirr+LGF). The remaining rats (Cirr) received saline. The groups were compared in terms of serum enzymes, tissue damage, total liver collagen, collagenase activity, microsomal enzyme activities, splanchnic and systemic hemodynamics and portosystemic shunting. RESULTS: Treatment of rats presenting CCl4-induced cirrhosis with Liver Growth Factor decreased serum aminotransferase levels and increased levels of serum albumin and total protein. The Liver collagen content was lower in rats treated with Liver Growth Factor (2.96 vs. 4.32 mg/g liver, p<0.01). Microscopic studies revealed that the livers of rats receiving Liver Growth Factor showed decreases in fibrosis, necrosis and inflammatory infiltration, as well as a recovery of architectural integrity. Liver function was improved after treatment with Liver Growth Factor, as indicated by the rate constant for elimination of aminopyrine, which increased from 0.0063 to 0.0170 (p<0.05). This increase was accompanied by a higher total amount of cytochrome P-450 as well as of certain P-450 isoenzymes, especially those that are hormone-dependent, such as P-450 3A. The improved liver histology and function observed in Cirr+LGF rats was associated with decreases in portal pressure (14.4 vs. 9.4 mm Hg, p<0.01) and portosystemic shunting (55.8 vs. 11.5%, p<0.01), as well as increases in mean arterial pressure and systemic vascular resistance, and a reduction in ascites. CONCLUSIONS: Administration of the hepatic mitogen, Liver Growth Factor, to CCl4-cirrhotic rats decreased liver collagen and reorganized the hepatic extracellular matrix, resulting in an improvement in liver function, reduced portal pressure and amelioration of ascites.


Subject(s)
Bilirubin/pharmacology , Carbon Tetrachloride Poisoning/drug therapy , Carbon Tetrachloride Poisoning/physiopathology , Growth Substances/pharmacology , Hemodynamics/drug effects , Liver Cirrhosis, Experimental/drug therapy , Liver Cirrhosis, Experimental/physiopathology , Serum Albumin/pharmacology , Aminopyrine/metabolism , Animals , Bilirubin/administration & dosage , Blood Proteins/analysis , Carbon Tetrachloride Poisoning/pathology , Collagen/analysis , Cytochrome P-450 Enzyme System/analysis , Growth Substances/administration & dosage , Inflammation , Injections, Intraperitoneal , Liver/chemistry , Liver/metabolism , Liver/pathology , Male , Necrosis , Portal System/physiology , Portasystemic Shunt, Surgical , Rats , Rats, Wistar , Serum Albumin/administration & dosage , Serum Albumin/analysis , Serum Albumin, Human , Transaminases/blood
SELECTION OF CITATIONS
SEARCH DETAIL
...