The Contribution of Mediastinal Transbronchial Nodal Cryobiopsy to Morpho-Histological and Molecular Diagnosis.

(1) Background: endobronchial ultrasound-guided mediastinal transbronchial cryo-node biopsy, previously assisted by fine-needle aspiration, is a novel technique of particular interest in the field of lung cancer diagnosis and is of great utility for extrathoracic tumor metastases, lymphomas, and granulomatous diseases. An integrated histological and molecular diagnosis of small samples implies additional difficulty for the pathologist. Additionally, emerging tumor biomarkers create the need to search for new approaches to better manage the tissue sample; (2) Methods: An analytical observational study of 32 mediastinal node cryobiopsies is carried out in 27 patients (n = 27). Statistical analysis using the t-student and Wilcoxon signed-rank tests for paired data is performed with SPSS 26 and R Statistical software. The significance level is established at p < 0.05; (3) Results: cryobiopsies were valid for diagnosis in 25 of 27 patients, with a maximum average size of 3.5 ± 0.7 mm. A total of 18 samples (66.67%) were positive for malignancy and 9 (33.33%) were benign. The tumor percentage measured in all neoplastic samples was greater than 30%. The average DNA and RNA extracted in nine non-small cell lung cancer cases was 97.2 ± 22.4 ng/µL and 26.6 ± 4.9 ng/µL, respectively; (4) Conclusions: the sample size obtained from an endobronchial ultrasound-guided mediastinal transbronchial cryo-node biopsy facilitates the morphological and histo-architectural assessment of inflammatory and neoplastic pathology. It optimizes molecular tests in the latter due to more tumor cells, DNA, and RNA.

Submucosal infiltrate of anisakis larvae. Rare cause of intestinal obstruction.

Patient aged 71 with a history of type 2 diabetes mellitus. He came to the emergency department for abdominal pain and vomiting. Laboratory tests showed an increase in acute phase reactants. Abdominal CT scan showed dilated jejunal loops, compatible with intestinal occlusion. Urgent intervention was performed, resecting the affected segment. The pathology report showed a prominent transmural inflammatory infiltrate and interstitial oedema, with moderate villous atrophy, identifying parasitic structures compatible with anisakis larvae (family Anisakidae). Given the mechanism of tissue invasion, the larvae are surrounded by a predominantly eosinophilic inflammatory infiltrate, organised as granulomas or abscesses.

A case report of hepatic angiomyolipoma, inflammatory subtype. Clinico-pathological characterization: a diagnostic challenge.

BACKGROUND: Angiomyolipoma is a benign mesenchymal neoplasm of a wide histological heterogeneity belonging to the PEComa "family." The liver, after the kidney, is their second most frequent location. However, inflammatory hepatic AMLs constitute a rare entity, with only fourteen documented cases until 2020. These neoplasms can overlap morphological features of IgG4-related diseases, being of great diagnostic relevance to demonstrating myomelanocytic-lineage differentiation of the neoplastic cells. CASE PRESENTATION: we present a new case of an inflammatory hepatic AML resembling an IgG4-related disease in a 35-year-old woman with a subcapsular 5 cm mass confined to segment VII of the right hepatic lobe. Although having reduced its size along the tumor's natural evolution, complete tumor resection was decided due to its hypermetabolic behavior (max. SUV = 12,6) assessed by PET-CT scan. Finally, the patient underwent a right hepatectomy due to spontaneous rupture and bleeding of the lesion during the intervention. All the diagnostic and therapeutic procedures occurred in the last months of the COVID-19 pandemic. CONCLUSIONS: This review aims to describe inflammatory hepatic AML histological and immunohistochemical features. We further sought to establish a clinicopathological contextualization of this tumoral subtype.

La implementación de Twitter e Instagram como herramientas complementarias durante las prácticas presenciales de histología / The implementation of Twitter and Instagram as complementary tools during face-to-face practical lessons of Histology

Introducción: La pandemia de la COVID-19 ha multiplicado el uso de redes sociales en la enseñanza médica, tanto de pregrado como de posgrado, debido a las restricciones sanitarias impuestas. Nuestro objetivo fue implementar el uso de Twitter e Instagram en las prácticas de histología para superar dichas limitaciones.Material y métodos.Se describe la utilización de Twitter e Instagram para el estudio práctico de histología tanto dentro como fuera del aula. Tras la finalización de las prácticas, los alumnos cumplimentaron una encuesta de satisfacción vía Google Form, cuyos resultados se sometieron posteriormente a análisis estadístico.Resultados.La encuesta fue completada por 261 alumnos. Un 86,6% pensó que el uso de estas redes sociales mejoró la dinámica global de la práctica. Asimismo, los alumnos percibieron que, gracias a estas herramientas, su nivel de implicación (70,9%) y atención (59,5%) aumentó durante las practicas. Finalmente, un 63,1% consideró muy útiles para su estudio las publicaciones subidas por el profesorado a Instagram.Conclusión.Twitter e Instagram constituyen recursos muy útiles para la enseñanza práctica en histología, ya que los alumnos se muestran bastante satisfechos y motivados con el uso de estas redes sociales para su aprendizaje. (AU)

Introduction: COVID-19 pandemic has doubled the use of Social Networks in medical education, both at undergraduate and postgraduate level, due to the sanitary restrictions affecting social distance. For that reason, we decided to implement the use of Twitter and Instagram during histological practical lessons in order to overcome these limitations.Material and methods.Use of Twitter and Instagram for practical learning in Histology both inside and outside the classroom. After the practical lessons, students filled a satisfaction survey via Google Forms, whose results were then analyzed statistically.Results.The survey was filled by 261 students. 86,6% of them thought that the use of social networks improved the global dynamics of the practical lessons. In the same way, most of the students perceived that thanks to these tools their implication (70,9%) and attention (59,5%) increased during the practices. Finally, 63,1% considered very useful for their study the Instagram posts uploaded by professors.Conclusion.Twitter and Instagram arise as useful resources for practical education in Histology since students appear to be satisfied and motivated with the use of these social networks for their learning. (AU)

Ultrasonographic and Histological Correlation after Experimental Reconstruction of a Volumetric Muscle Loss Injury with Adipose Tissue.

Different types of scaffolds are used to reconstruct muscle volume loss injuries. In this experimental study, we correlated ultrasound observations with histological findings in a muscle volume loss injury reconstructed with autologous adipose tissue. The outcome is compared with decellularized and porous matrix implants. Autologous adipose tissue, decellularized matrix, and a porous collagen matrix were implanted in volumetric muscle loss (VML) injuries generated on the anterior tibial muscles of Wistar rats. Sixty days after implantation, ultrasound findings were compared with histological and histomorphometric analysis. The muscles with an autologous adipose tissue implant exhibited an ultrasound pattern that was quite similar to that of the regenerative control muscles. From a histological point of view, the defects had been occupied by newly formed muscle tissue with certain structural abnormalities that would explain the differences between the ultrasound patterns of the normal control muscles and the regenerated ones. While the decellularized muscle matrix implant resulted in fibrosis and an inflammatory response, the porous collagen matrix implant was replaced by regenerative muscle fibers with neurogenic atrophy and fibrosis. In both cases, the ultrasound images reflected echogenic, echotextural, and vascular changes compatible with the histological findings of failed muscle regeneration. The ultrasound analysis confirmed the histological findings observed in the VML injuries reconstructed by autologous adipose tissue implantation. Ultrasound can be a useful tool for evaluating the structure of muscles reconstructed through tissue engineering.