ABSTRACT
Type 2 transglutaminase (Tg), which catalyzes the covalent cross-linking of cytoplasmic proteins during apoptosis, also functions as the alpha subunit of a heterodimeric G-protein (Gh) which can activate phospholipase C-delta1 during the signal transduction pathway linked to alpha1-adrenoreceptors. Continued stimulation of rat forebrain ventricular zone (VZ) germinal cells with the alpha1-agonist phenylephrine during development in vitro suppresses apoptosis and promotes DNA synthesis [Pabbathi et al., Brain Res., 760, 1997, 22-33]. Immunocytochemistry with a monoclonal antibody to Galphah/Tg reveals that alpha1-agonist deprivation during culture of VZ cells in the presence of a protein synthesis inhibitor results after 20 h in a loss of peripheral distribution of the protein and an increase in the reaction product of Tg in the cytoplasm of cells undergoing apoptosis. Using photoaffinity labelling, we observed reduced GTP binding to Galphah/Tg in phenylephrine-deprived cultures. Formation of inositol triphosphate (IP3) and intracellular Ca2+ transients occurred in the presence of phenylephrine. In cultures grown in phenylephrine-deprived conditions in the presence of protein synthesis inhibitor, both the IP3 response and the amplitude and duration of Ca2+ transients were reduced. These results show that loss of signal transduction coincides with the onset of transglutaminase activity in VZ cells during a period when cell survival is reduced following withdrawal of alpha1-agonist, and support the hypothesis that Tg/Galphah could be implicated in both signal transduction and programmed cell death.
Subject(s)
Catecholamines/deficiency , Cerebral Ventricles/physiology , Cytosol/enzymology , GTP Phosphohydrolases/analysis , Receptors, Adrenergic, alpha-1/physiology , Signal Transduction/physiology , Transglutaminases/analysis , Animals , Apoptosis/physiology , Cells, Cultured , Cerebral Ventricles/cytology , Cerebral Ventricles/enzymology , DNA/biosynthesis , GTP-Binding Proteins/metabolism , Immunohistochemistry , Protein Glutamine gamma Glutamyltransferase 2 , RatsABSTRACT
One hundred informants including full time/part time herbalists, old ladies, family heads and village heads were interviewed for the type of plants used in their homes for the treatment of some common diseases. Forty eight plant species were found to be used by Ondo people in folk medicine. Fourteen species were found to contain alkaloids and 34 plant species were found to have local actions. The plant parts used, their uses and biodynamic notes are given.
Subject(s)
Medicine, Traditional , Plants, Medicinal , Humans , NigeriaSubject(s)
Job Satisfaction , Physicians/supply & distribution , Rural Health , Adult , Aged , Health Services , Humans , Life Style , Middle Aged , Missouri , Osteopathic Medicine , Physician-Patient Relations , Urban Population , WorkforceABSTRACT
The study examines changes in location of osteopathic and medical doctors in a 20-county area of rural Missouri over a 14-year period. Losses of osteopathic physicians were greater than medical doctors. However, there was a convergence over the 14-year period in background characteristics of the two types of physicians. The finding of greater spatial mobility of DOs is placed in the context of professional mobility of osteopathy. It is also argued that as practice opportunities for DOs increase, background factors associated with early socialization become more influential in choice of practice sites. The relationship of practice opportunities to choice of practice sites can be extended to foreign medical school graduates and "new health practitioners."
