[The dental status of patients with gastroesophageal reflux disease depending on the intake of proton pump inhibitors]. / Stomatologicheskii status patsientov s gastroezofageal'noi reflyuksnoi bolezn'yu v zavisimosti ot priema ingibitorov protonnoi pompy.

The number of men and women with gastroesophageal reflux disease (GERB) is increasing each year; besides, the disease seeks rejuvenation. Because the oral cavity is the initial digestive system, gastrointestinal tract diseases (GT) and associated changes in the oral cavity are closely related. THE AIM OF THE STUDY: Was to assess the dental status of patients with gastroesophageal reflux disease depending on the use of proton pump inhibitors based on the comprehensive clinical and laboratory study. The study involved 100 people aged between 35-65 years, which were divided into 3 groups (30 people - I control group, 35 - II patients with GERD who did not take proton pump inhibitors and 35 - III patients with GERD who take proton pump inhibitors). To detect patients with a predisposition to GERB, all participants in the study answered the «GRDQ¼ questionnaire. The patient was referred to a gastroenterologist for consultation, if the final score was more than 8 points. To identify the dental status of the patients, the DMFT (Decayed/Missing/Filled Teeth) index and the periodontal PI index were determined, the pH of the saliva was measured, and also the PCR (polymerase chain reaction) method was used. According to the results of the study, the final score of the GRDQ questionnaire in group II was significantly higher than that of group I and group III. The study revealed a high level of caries intensity, deterioration of periodontal and hygienic status, high prevalence of periodontal pathogens: Porphyromonas gingivalis, Prevotella intermedia and Actinobaccilus actinomecetemcomitans in patients of group II, compared with patients of group III and the control group. As a result of the study of the pH of mixed saliva, an acid shift was observed in group II. Dental status in patients with gastroesophageal reflux disease worsens.

[Сlinicmolecular indicators of inflammatory destructive damage of the oral cavity in periodontitis in persons with various group accessories of blood.]

In order to find a connection between the alteration of oral tissues and genetic predisposition to inflammatory and destructive processes in oral media, the cytokine profile of the oral fluid of clinically healthy individuals was determined for various blood group affiliations according to the AB0 system. The group-specific features of individuals with B(III) blood group were revealed: an increase of 32,5% in the content of interleukin-6 and 63,1% in the content of interleukin-8 compared with similar data for people with 0(I), A(II), AB(IV) blood groups, which can predispose to the greatest activity of the inflammatory process in the oral cavity in individuals with antigen B. Confirmation of this fact is an increase of IgA antibodies to gliadin in the blood among patients with chronic generalized periodontitis with B(III) blood group, up to 5,00 U/ml (p<0,01), which indicates the processes of acute inflammation, and along with an increase in blood IgG antibodies to transglutaminase in comparison with a group of clinically healthy individuals, it serves as an indicator of damage to the body's connective tissue at the molecular level. When examining the dental status, pronounced clinical manifestations of chronic generalized periodontitis were found in patients with A(II) blood group, the molecular foundation of which is the highest content of IgA and IgG antibodies to transglutaminase in the oral fluid (0,35 U/ml and 0,45 U/ml), which contributes to the activation of periodontal-destroying inflammatory and inflammatory processes, obviously, with a tendency to the chronic course of the disease. The studies performed allowed us to analyze in clinically healthy individuals a predisposition to alternative processes in oral environments, using gradation by group blood affiliation.

[Coagulation test features depending on the AB0-blood groups system antigenic composition.]

The maintenance of normal blood flow through the vessels is the result of the coordinated work of the coagulation and anticoagulation systems of our body. The balance of this system depends on many factors, including endothelial, humoral, platelet ones, however, we still lack knowledge about the effect of antigenic determinants on the state of the hemostatic system. This study is devoted to assessing the effect of the presence and absence of antigens on the AB0 system, presented on erythrocyte and platelet membranes, on hemostatic parameters. The study was conducted in the Clinics of Samara State Medical University and consisted of127 clinically healthy individuals who underwent a general analysis and biochemical blood analysis, 52 people with the most stable indicators of cell composition and metabolic profile were selected for a coagulation test, including determination of the activity of coagulation factors and routine tests. A significant decrease in the activity of the VIII and VII coagulation factors was revealed, as well as an increase in the prothrombin time in patients with 0 (I) blood group compared to the "antigenic" blood groups. The presence of biological variation for indicators of external and internal coagulation paths was noted, depending on the group of blood belonging to the AB0 system. The findings suggest that there is an increased susceptibility to bleeding in patients with 0 (I) blood groups due to the absence of antigenic determinants on the cell membrane, while for "antigenic" blood groups, on the contrary, there is a susceptibility to thrombosis due to increased activity of the components of the coagulation system.

[Molecular markers of the oral mucosa damage in patients with leukemia.]

The criteria for early manifestations of mucosal lesions in patients with acute and chronic leukemia are distinguished: in the absence of complaints and changes in the index assessment of the oral cavity, a block of initial dental signs in the form of edema of the tongue and the smoothness of its papillae, pallor of the oral mucosa in patients with leukemia. In theoral fluid of patients with the initial signs of dental pathology in acute leukemia, a specific spectrum of disorders was established in the form of the lowest antibody level of immunoglobulin A class to transglutaminase in combination with a high amount of Ig G antibodies to transglutaminase - 8.73 ± 0.92 U / ml, which maximal level exceeds the reference limits by almost 4 times (38.80 U / ml) - that indicates structural changes in the connective tissues of the oral cavity. Under the influence of chemotherapy in these patients, the inversion of clinical manifestations of dental pathology was noted: stomatitis II (medium) severity is formed, bypassing I degree, accompanied by a 4-fold increase of IgA antibodies to transglutaminase (4.03 ± 0, 77 U / ml, p < 0.05) in the oral fluid of patients with acute leukemia and 2.5 times for chronic leukemias (3.24 ± 0.47 U / ml, p < 0.05) compared to pre-treatment period. In this regard, it is recommended to determine the content in the oral fluid for antibodies to transglutaminase in patients with leukemia for verification of the degree of inflammatory-destructive process and its recovery. The peculiarity of immunological shifts in stomatitis of the 1st degree of severity is the highest level of IgG antibodies to transglutaminase (9.98 ± 1.50 U / ml) in the oral fluid, and at II-III degree the molecular manifestations of damage are smoother, which indicates the depression of immune processes.