ABSTRACT
Additively manufactured lattices have been adopted in applications ranging from medical implants to aerospace components. For solid AM components, the effect of build parameters has been well studied but comparably little attention has been paid to the influence of build parameters on lattice performance. For this project, the main aim was to evaluate static compressive mechanical performance of regular and stochastic lattices as a function of build parameters. The second aim was to compare strut dimensions of the metal lattice structures as build parameters were changed. Both regular and stochastic lattices were fabricated with a designed strut diameter of either 200 µm or 300 µm on a laser powder bed fusion machine. A range of laser power (140-180 W), scan speed (1700-2100 mm/s), and laser offset (0-45 µm) were used in fabricating each lattice. Compression tests were performed following the ISO 13314 (2011) standard to measure modulus, yield strength, and ultimate compressive strength values. Laser power adjustments produced the most significant effect on lattice performance. A change of 50 W resulted in roughly a 2X increase in maximum load and modulus for both regular and stochastic lattice structures. Regular lattice structures had a higher mechanical response during the mechanical evaluation. Internal strut diameters varied between build parameters as well, with laser offset adjustments producing the most noticeable change in strut geometry between lattice samples. These findings suggest that build parameter optimization, in lieu of using OEM parameters developed for solid structures, is necessary to ensure the optimum mechanical performance of AM lattice structures.
Subject(s)
Prostheses and Implants , Titanium , Titanium/chemistry , Materials Testing , Porosity , Stress, MechanicalABSTRACT
BACKGROUND: Additive manufacturing (AM), commonly called 3D Printing (3DP), for medical devices is growing in popularity due to the technology's ability to create complex geometries and patient-matched products. However, due to the process variabilities which can exist between 3DP systems, manufacturer workflows, and digital conversions, there may be variabilities among 3DP parts or between design files and final manufactured products. The overall goal of this project is to determine the dimensional variability of commercially obtained 3DP titanium lattice-containing test coupons and compare it to the original design files. METHODS: This manuscript outlines the procedure used to measure dimensional variability of 3D Printed lattice coupons and analyze the differences in external dimensions and pore area when using laser and electron beam fabricated samples. The key dimensions measured were the bulk length, width, and depth using calipers. Strut thickness and pore area were assessed for the lattice components using optical imaging and µCT. RESULTS: Results show a difference in dimensional measurement between printed parts and the computer-designed files for all groups analyzed including the internal lattice dimensions. Measurements of laser manufactured coupons varied from the nominal by less than 0.2 mm and results show averages greater than the nominal value for length, width, and depth dimensions. Measurements of Electron Beam Melting coupons varied between 0.4 mm-0.7 mm from the nominal value and showed average lengths below the nominal dimension while the width and depths were greater than the nominal values. The length dimensions of Laser Powder Bed Fusion samples appeared to be impacted by hot isostatic press more than the width and depth dimension. When lattice relative density was varied, there appeared to be little impact on the external dimensional variability for the as-printed state. CONCLUSIONS: Based on these results, we can conclude that there are relevant variations between designed files and printed parts. However, we cannot currently state if these results are clinically relevant and further testing needs to be conducted to apply these results to real-world situations.
ABSTRACT
Heme oxygenase (HO), the enzyme responsible for heme catabolism, has been associated with the function of both skeletal and smooth muscle cells and with protection of the heart against ischemia/reperfusion injury. Exposure of skeletal muscle cultures to heme, the physiological substrate for HO, has been shown to improve differentiation and aerobic metabolism. Little is known, however, about the roles that heme and heme metabolism play in cardiac muscle, and the present study was conducted to examine the effects of exogenous heme on cultured heart cells in the presence or absence of modulators of HO activity. Treatment of neonatal rat ventricular cells with heme resulted in increases in four key indicators: (1) the activity of metabolic enzymes, (2) the rate of spontaneous contraction, (3) the level of myosin heavy chain (MyHC) expressed, and (4) the amount of actin organized as filaments. Treatment with heme while metabolically inhibiting increased HO activity altered these effects such that: (1) increases in enzyme activities were attenuated, (2) spontaneous beating ceased, (3) the level of MyHC was reduced, and (4) the amount of filamentous actin was severely decreased to the point where myofibrils were no longer evident. These results suggest that heme and its catabolites act to modulate aspects of cardiac cell function and organization.
