[Selection and biochemical activity of a microbial biocenosis in the process of concentrated sewage treatment]. / Selektsiia i biokhimicheskaia aktivnost' mikrobnogo soobshchestva v protsesse ochistki kontsentrirovannykh stochnykh vod.

A bacterial cenosis responsible for the destruction of organic wastes having a complex composition was selected under the conditions of continuous cultivation on highly concentrated industrial sewage. Most of the bacterial species and all of the yeasts and fungi were eliminated from the original microflora when the concentration of the purified sewage was increased from 2 to 12 g O2 per litre (in terms of the overall chemical oxygen uptake, COU) and the dilution rate (D) varied from 0.08 to 0.25 h-1. The number of species was reduced to 10 in the cenosis. The degree of oxidation of organic compounds in the sewage was 94 to 97% at the maximal parameters of the process. The specific rate of oxidation as well as the respiration and dehydrogenase activities increased with a rise in both COU and D. The highest biochemical activity was observed at D=0.25 h-1:

[Amino acid makeup characteristics of bacteria utilizing nonnatural compounds]. / Osobennosti aminokislotnogo sostava u bakterii, utiliziruiushchikh neprirodnye soedineniia.

A peculiarity of the amino acid pool has been discovered for the first time in bacteria utilizing such synthetic compounds as gamma butyrolactam, epsilon-caprolactam and zeta-aminoenanthic acid. The main components of the amino acid pool are omega-aminoacids (including synthetic ones) as well as glutamic acid. The total amino acid content increases upon utilization of the compounds being tested three times (Pseudomonas fluorescens), five times (Pseudomonas dacunchae) and seven times (Pseudomonas perolens) as compared to the control variants. These data suggest that hydrolysis and transamination of synthetic omega-amino acids play an important role in the constructive and energy metabolism of the bacteria under study.