ABSTRACT
Pyrrolo[1,2-a][1,4]benzodiazepin-4-one (1), -6-one (2), and -4,6-dione (3), which are starting materials for the synthesis of pharmacologically interesting compounds that are active as neurotropic agents, have been characterized in the gas phase. The application of different mass spectrometric techniques, such as electron ionization, high-resolution, and tandem mass spectrometry, has allowed the structural characterization and differentiation of their molecular ions and most abundant fragment ions formed in the source. In particular, the two positional isomers 1 and 2 produce quite different mass spectra, and their molecular and the most intense fragment ions yield different metastable mass-analyzed ion kinetic energy spectra. Furthermore, high-resolution mass spectrometry and accurate mass measurements have revealed different elemental compositions and abundances for isobaric fragment ions produced by isomers 1 and 2. From these data and from the comparison with those relevant to compound 3, it has been possible to evaluate the influence of the position of the carbonyl group on the fragmentation pathways.Semiempirical molecular orbital calculations carried out by both the modified neglect of differential overlap and Austin 1 methods have provided useful information on the characterization of the neutrals as well as the molecular ions of compounds 1-3.
