ABSTRACT
The gyrogen with tubes has been the object of repeated trials carried out over a period of some years. The aim of these trials was essentially to multiply BHK cells in monolayer and to reproduce the FMD virus in one operation. At the laboratory stage, the gyrogen consisted of 3 rolling flasks; at the pilot stage, it equalled 100 rolling flasks; and at the industrial stage which will be tested in the coming months, it should equal 500 flasks. The culture of cells on a glass support was not in itself new; the difficulty appeared when replicating the virus in order to obtain the high parameters necessary for the production of vaccine. Everything was done so that finally cell culture and viral multiplication could be carried out in situ in the same apparatus.
Subject(s)
Culture Techniques/instrumentation , Animals , Aphthovirus/growth & development , Cell Line , Cricetinae , Culture Techniques/methods , Kidney , Virus CultivationABSTRACT
The study of the plaques produced by viruses Asia and O1 reveals different properties which may depend on the virus or on the cell or its method of culture. Antigenic differences and subsequent immunological differences correspond to differences in plaques. On the other hand, the cell line in suspension evolves both in its morphology and its receptivity during the passages. All these variations imply the observations of quantitative and qualitative criteria of evaluation in order to prepare vaccines from viruses similar to viruses specific to a given epizooty.
