ABSTRACT
The interest in algae-derived bioactive compounds has grown due to their potential therapeutic efficacy against a range of diseases. These compounds, derived from proteins, exhibit diverse functions and profound pharmacological effects. Recent research has highlighted the extensive health benefits of algae-derived bioactive compounds, positioning them as potential natural antioxidants in the food, pharmaceutical, and cosmetic industries. This study focuses on extracting proteins from Porphyra yezoensis using innovative physical pre-treatment methods such as stirring, ball milling, and homogenization, under various acidic and alkaline conditions. Enzymatic hydrolysis, employing commercial enzymes at optimal temperature, pH, and enzyme-substrate ratios, produced distinct fractions according to molecular weight. Pepsin demonstrated the highest hydrolysis rate, with the fraction above 10 kDa identified as the most bioactive hydrolysate. Antioxidant activity was evaluated through DPPH, ABTS, ferrous ion chelation, and reducing power assays, demonstrating high antioxidant potential and the ability to mitigate oxidative stress. The 10 kDa fraction of pepsin hydrolysate exhibited 82.6% DPPH activity, 77.5% ABTS activity, 88.4% ferrous ion chelation activity, and higher reducing power potential (0.84 absorbance at 700 nm). Further exploration of mechanisms, amino acid profiles, and potential in vivo benefits is essential to fully exploit the medicinal potential of these algae-derived hydrolysates.
ABSTRACT
The escalating demand for long-chain polyunsaturated fatty acids (PUFAs) due to their vital health effects has deepened the exploration of sustainable sources. Thraustochytrium sp. stands out as a promising platform for omega-3 and 6 PUFA production. This research strategically optimizes key parameters: temperature, salinity, pH, and G:Y:P ratio and the optimized conditions for maximum biomass, total lipid, and DHA enhancement were 28 °C, 50 %, 6, and 10:1:2 respectively. Process optimization enhanced 32.30 and 31.92 % biomass (9.88 g/L) and lipid (6.57 g/L) yield. Notably, DHA concentration experienced a substantial rise of 69.91 % (1.63 g/L), accompanied by notable increases in EPA and DPA by 82.69 % and 31.47 %, respectively. MANOVA analysis underscored the statistical significance of the optimization process (p < 0.01), with all environmental factors significantly influencing biomass and lipid data (p < 0.05), particularly impacting DHA production. Thraustochytrium sp. can be a potential source of commercial DHA production with the fine-tuning of these key process parameters.
