ABSTRACT
A series of 34 cases of soft tissue tumours of possible muscle origin was studied immunohistochemically using different antibodies. Tissue specimens and/or aspirates from fine needle aspiration biopsy (FNAB) were examined. The results indicate that immunohistochemistry is a valuable tool in differential diagnosis of soft tissue tumours, improving diagnostic accuracy in 83.5% of the examined cases. Desmin expression confirmed muscle differentiation of tumours in 47.6% of diagnostically difficult cases. The application of immunohistochemistry may be especially helpful in rendering the diagnosis of tumour type more precise when a needle aspirate is the only available diagnostic material.
Subject(s)
Immunohistochemistry/methods , Soft Tissue Neoplasms/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Desmin/analysis , Diagnosis, Differential , Female , Humans , Infant , Male , Middle Aged , Vimentin/analysisABSTRACT
The clinico-morphological features in 13 patients (nine female) with neuroendocrine carcinoma of the skin are presented. The mean age was 64.9 years. The limbs were the most common site of primary tumour, followed by the face. The clinical course was characterized by a high incidence of regional lymph node metastases (69%) and recurrences (46%). Seven of the patients died of tumour, with a mean survival time of 13 months. Histologically, a solid pattern of tumour growth was most common. The cells were usually small and uniform. Squamous cell differentiation was found in one tumour. The cell of all tumours reacted positively for cytokeratins and neuron-specific enolase. The positive reaction frequently had a ball-like globular pattern, corresponding to inclusion-like bodies seen on light microscopy and to paranuclear whorls of intermediate filaments observed on electron microscopy. Neurosecretory granules were seen on electron microscopy in the 11 cases examined and in one case a 'Luse body' was found in the intercellular space.
Subject(s)
Carcinoma, Merkel Cell/pathology , Skin Neoplasms/pathology , Aged , Carcinoma, Merkel Cell/diagnosis , Carcinoma, Merkel Cell/metabolism , Cytodiagnosis , Female , Humans , Immunohistochemistry , Inclusion Bodies/pathology , Keratins/metabolism , Male , Microscopy, Electron , Middle Aged , Phosphopyruvate Hydratase/metabolism , Skin Neoplasms/diagnosis , Skin Neoplasms/metabolismABSTRACT
The light and transmission electron microscopic findings and the intermediate filament typing of tumor cells from fine needle aspirates of primary, recurrent and metastatic neuroendocrine (Merkel-cell) carcinoma of the skin are described. The tumor cells in the smears coexpressed keratins and neurofilaments and were characterized by "intermediate filament buttons," i.e., buttonlike fragments of cytoplasm of tumor cells, which could be observed either by staining with intermediate filament-specific antibodies using immunofluorescence or by their appearance in hematoxylin-and-eosin-stained smears. These features may help in the differential diagnosis of fine needle aspirates of neuroendocrine carcinomas of the skin.
Subject(s)
Adenocarcinoma/ultrastructure , Carcinoid Tumor/ultrastructure , Cytoskeleton/ultrastructure , Intermediate Filaments/ultrastructure , Skin Neoplasms/ultrastructure , Adenocarcinoma/diagnosis , Adenocarcinoma/secondary , Aged , Antibody Specificity , Antigens, Surface/analysis , Antigens, Surface/immunology , Biopsy, Needle/methods , Carcinoid Tumor/diagnosis , Carcinoid Tumor/secondary , Diagnosis, Differential , Fluorescent Antibody Technique , Histocytochemistry , Humans , Keratins/analysis , Keratins/immunology , Male , Microscopy, Electron , Skin Neoplasms/diagnosis , Skin Neoplasms/secondarySubject(s)
Chemical Industry , Dermatitis, Occupational/chemically induced , Scleroderma, Localized/chemically induced , Vinyl Chloride/adverse effects , Vinyl Compounds/adverse effects , Adult , Dermatitis, Occupational/pathology , Female , Humans , Male , Middle Aged , Scleroderma, Localized/pathologySubject(s)
Skin/ultrastructure , Animals , Cattle , Hair Removal , Microscopy, Electron, Scanning , SwineABSTRACT
The distribution of 99mTc-Unithiol complex was studied by macro-autoradiography in rats and in rabbit kidneys, and the presence of activity in the renal cortex was easily demonstrated. Using micro-autoradiography, the accumulation of activity in various parts of the nephron was observed to concentrate in the cells of the proximal and distal tubuli to a larger extent than in the glomeruli.
