ABSTRACT
A Methanol Extraction Residue (MER) of BCG tubercle bacillus induced cytostatic activity in vitro against the murine tumor-cell lines YAC-1 and MOPC-315 in resident murine peritoneal macrophages isolated from BALB/c mice. The induction of antitumor activity was not associated with increase in release of TNF-alpha. Macrophages from mice hyperimmunized with MER (MER 3x) or from mice injected once with MER in aqueous suspension released more PGE2 following stimulation in vitro with LPS. Macrophages cultured with either MER or MER+LPS prolonged the survival time of mice bearing palpable RPC5 plasmacytoma s.c. tumors.
Subject(s)
Macrophages, Peritoneal/immunology , Mycobacterium bovis/immunology , Neoplasms, Experimental/therapy , Animals , Cells, Cultured , Immunotherapy , Lipopolysaccharides/pharmacology , Macrophage Activation , Male , Mice , Mice, Inbred BALB C , Neoplasms, Experimental/immunology , Plasmacytoma/therapy , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
The Nocardia fraction NLD-RB1040 (Nocardia Lysozyme Digest) induced cytostatic activity in vitro against the YAC-1 tumor-cell line, in resident murine peritoneal macrophages isolated from BALB/c mice. The induction of antitumor activity by the Nocardia fraction was not correlated with induced changes in production of TNF alpha, PGE2 or nitrite by human peritoneal macrophages collected from renal patients on Continuous Ambulatory Peritoneal Dialysis (CAPD), or changes in production of TNF a by murine macrophages.
Subject(s)
Macrophage Activation , Macrophages, Peritoneal/immunology , Nocardia , Tumor Necrosis Factor-alpha/biosynthesis , Animals , Antineoplastic Agents , Cells, Cultured , Dinoprostone/metabolism , Female , Humans , Lipopolysaccharides/pharmacology , Macrophage Activation/drug effects , Macrophages, Peritoneal/drug effects , Mice , Mice, Inbred BALB C , Nitrites/metabolism , Peritoneal Dialysis, Continuous Ambulatory , Recombinant Proteins/analysis , Tetradecanoylphorbol Acetate/pharmacology , Tumor Necrosis Factor-alpha/analysisABSTRACT
Previously reported studies revealed that extensive immunization with the Methanol Extraction Residue (MER) of BCG tubercle bacillus in Incomplete Freund's Adjuvant (IFA) induced marked suppression of T and B cell functions and that immunosuppression was correlated with marked decrease in splenic T-cell population and marked increase in the splenic macrophage population. The purpose of the present work was to determine if extensive immunization with MER in IFA also induced changes in splenic asialo antigen positive (NK) population and in the potential of induction of splenic LAK activity in vitro by recombinant IL-2. By comparison with other test groups, namely mice injected with IFA only, injected once with MER in aqueous suspension and normal, untreated mice, hyperimmunization with MER resulted in marked increase in expression of asialo positive (NK) splenic cells whereas induction of splenic LAK activity by IL-2 was markedly depressed. Splenic macrophages originated from MER hyperimmunized mice had no effect on induction of LAK activity by IL-2 in a normal splenic population.
