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1.
Am J Physiol Heart Circ Physiol ; 281(6): H2680-6, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11709437

ABSTRACT

Several disease states, including hypertension, are associated with elevations in plasma endothelin-1 (ET-1) and variable changes in vascular contraction to ET-1. The spotting lethal (sl) rat carries a deletion of the endothelin-B (ET(B)) receptor gene that prevents expression of functional ET(B) receptors, resulting in elevated plasma ET-1. On a normal diet, these rats are normotensive and thus provide an opportunity to study the vascular effects of chronically elevated ET-1 in the absence of hypertension. Studies were performed in rats homozygous for the ET(B) deficiency (sl/sl; n = 8) and in transgenic rats heterozygous for the ET(B) deficiency (sl/+; n = 8). Plasma ET-1 was elevated in sl/sl rats (3.85 +/- 0.55 pg/ml) compared with sl/+ rats (0.31 +/- 0.11 pg/ml). Mean arterial blood pressure in conscious unrestrained sl/sl and sl/+ rats was 101 +/- 5 and 107 +/- 6 mmHg, respectively. Concentration-dependent contractions to ET-1 (10(-11)-10(-8) M) were reduced in mesenteric small arteries (150-250 microm) from sl/sl rats, as indicated by an approximately 10-fold increase in EC(50). A selective ET(A) antagonist, A-127722 (30 nM), abolished contraction to ET-1 in both groups, whereas a selective ET(B) antagonist had no effect. Also, ET(B) agonists (IRL-1620 and sarafatoxin 6c) produced neither contraction nor relaxation in either group, indicating that contraction to ET-1 in this vascular segment was exclusively ET(A) dependent. Despite increased plasma ET-1, protein expression of ET(A) receptors in membrane protein isolated from mesenteric small arteries was increased in sl/sl compared with sl/+ rats, as shown by Western blotting. These results indicate that, in ET(B)-deficient rats, ET(A)-induced contraction is reduced in vessels normally lacking ET(B)-mediated effects. Reduced contraction may be related to elevated plasma ET-1 and occurs in the presence of increased ET(A) receptor protein expression, suggesting an uncoupling of ET(A) receptor expression from functional activity.


Subject(s)
Endothelin-1/pharmacology , Receptors, Endothelin/genetics , Receptors, Endothelin/metabolism , Vasoconstriction/drug effects , Vasoconstriction/physiology , Animals , Animals, Genetically Modified , Blood Pressure/drug effects , Blood Pressure/physiology , Blotting, Western , Dose-Response Relationship, Drug , Endothelin-1/blood , Heterozygote , Homozygote , Male , Mesenteric Arteries/chemistry , Mesenteric Arteries/physiology , Rats , Receptor, Endothelin A , Receptor, Endothelin B , Receptors, Endothelin/analysis , Vascular Resistance/drug effects , Vascular Resistance/physiology
2.
J Cardiovasc Pharmacol ; 36(6): 758-63, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11117376

ABSTRACT

Contraction of vascular smooth muscle by endothelin-1 is dependent on extracellular and intracellular Ca2+. However, the role of ryanodine-sensitive Ca2+ stores in endothelin-1-induced contraction is unknown. Vascular contraction was measured in mesenteric small arteries (200-300 microm intraluminal diameter) isolated from Sprague-Dawley rats and maintained at a constant intraluminal pressure of 40 mm Hg. The presence of functional ryanodine receptor Ca2+ release channels (RyRC) was demonstrated by the finding that ryanodine (10 microM), which locks the RyRC in a subconductance state, produced significant contraction of small arteries in the presence of 15 mM KCl. This effect was inhibited by dantrolene (10 microM), a RyRC inhibitor. Dantrolene significantly reduced the ET(A) receptor-mediated contraction to endothelin-1 (10(-11)-10(-9) M). The ability of dantrolene to reverse contraction induced by endothelin-1 was also determined. Dantrolene (1-10 microM) produced concentration-dependent relaxation of vessels precontracted to 38+/-3% of resting diameter with endothelin-1 but had no effect in vessels precontracted to a similar degree with phenylephrine or KCl. Because activation of RyRC may be dependent on production of cyclic ADP-ribose, the effect of nicotinamide (2 mM), an inhibitor of ADP-ribosyl cyclase, on contraction to endothelin-1 was determined. Nicotinamide had an inhibitory effect similar to that produced by dantrolene. A combination of nicotinamide and dantrolene had no greater effect than either agent alone, suggesting a common pathway for cyclic ADP-ribose and RyRC. In summary, endothelin-1 induces contraction of small mesenteric arteries through ET(A) receptor-mediated production of cyclic ADP-ribose and activation of RyRC.


Subject(s)
Adenosine Diphosphate Ribose/analogs & derivatives , Adenosine Diphosphate Ribose/pharmacology , Calcium Channels/drug effects , Endothelin-1/pharmacology , Mesenteric Arteries/drug effects , Muscle, Smooth, Vascular/drug effects , Ryanodine Receptor Calcium Release Channel/drug effects , Animals , Cyclic ADP-Ribose , Dantrolene/pharmacology , Muscle Relaxants, Central/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase Type III , Nitroarginine/pharmacology , Potassium Channel Blockers , Rats , Rats, Sprague-Dawley
3.
Am J Physiol Regul Integr Comp Physiol ; 279(2): R492-8, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10938237

ABSTRACT

Cyclic nucleotide-dependent vascular relaxation is associated with increases in the phosphorylation of a small heat shock protein (HSP), HSP20. An increase in phosphorylation of another small HSP, HSP27, is associated with impaired cyclic nucleotide-dependent vascular relaxation. Expression of HSPs is altered by exposure to several types of cellular stress in vitro. To determine if behavioral stress in vivo alters vascular expression and phosphorylation of the small HSPs and cyclic nucleotide-dependent vascular relaxation, borderline hypertensive rats were stressed by restraint and exposure to air-jet stress 2 h/day for 10 days or remained in their home cage. Stress impaired relaxation of aorta to forskolin, which activates adenylyl cyclase, and sodium nitroprusside, which activates guanylyl cyclase. This was associated with an increase in the aortic expression and phosphorylation of HSP27, which was localized to the vascular smooth muscle, but a decrease in the amount of phosphorylated (P)-HSP20. To determine if P-HSP27 inhibits phosphorylation of HSP20, P-HSP27 was added to a reaction mixture containing recombinant HSP20 and the catalytic subunit of cAMP-dependent protein kinase. P-HSP27 inhibited phosphorylation of HSP20 in a concentration-dependent manner. These data demonstrate that P-HSP27 can inhibit phosphorylation of HSP20. The increase in P-HSP27 and decrease in P-HSP20 were associated with reduced cyclic nucleotide-dependent vascular smooth muscle relaxation in response to behavioral stress in vivo, an effect similar to that observed previously in response to cellular stress in vitro.


Subject(s)
Heat-Shock Proteins/metabolism , Stress, Physiological/physiopathology , Vasodilation , Animals , Aorta/metabolism , Aorta/physiopathology , Female , HSP20 Heat-Shock Proteins , Hemodynamics , Male , Phosphoproteins/metabolism , Phosphorylation , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Stress, Physiological/metabolism , Tissue Distribution , Vasomotor System/physiopathology
4.
Am J Physiol ; 276(2): R435-40, 1999 02.
Article in English | MEDLINE | ID: mdl-9950922

ABSTRACT

Behavioral stress and aging are associated with an increase in vascular disease. This study determined the mechanisms contributing to changes in endothelium-dependent relaxation of isolated coronary arteries (300-350 micrometers) induced by exposure to 10 days of air-jet stress (2 h/day) in young (3 mo) and old (18 mo) male borderline hypertensive rats (BHR). Aging, alone, did not alter endothelium-dependent relaxation to acetylcholine (ACh) quantitatively but did alter the mechanisms contributing to relaxation to ACh, which was largely dependent on nitric oxide synthase (NOS) in vessels from old, but not young, BHR. Behavioral stress resulted in an enhanced relaxation to ACh that was dependent on NOS in vessels from young stressed compared with young control BHR. Conversely, relaxation to ACh was reduced in coronary arteries from old stressed compared with old control BHR. In vessels from old control BHR, there was an NOS-independent component of relaxation mediated by opening of K+ channels that was absent in vessels from old stressed BHR. The superoxide anion scavenger, tiron, partially restored relaxation, and inhibition of cyclooxygenase largely restored relaxation to ACh in vessels from old stressed BHR. In summary, the effect of behavioral stress was age dependent. ACh-induced relaxation of coronary arteries was enhanced in an NOS-dependent manner in young BHR and was impaired in old BHR due to superoxide anions, vasoconstrictor cyclooxygenase products, and a loss of K+ channel-mediated relaxation.


Subject(s)
Aging/physiology , Coronary Vessels/physiopathology , Hypertension/physiopathology , Stress, Psychological/physiopathology , Vasodilation/physiology , Acetylcholine/pharmacology , Animals , Arteries/physiopathology , Coronary Vessels/drug effects , Enzyme Inhibitors/pharmacology , Female , Male , Nitroarginine/pharmacology , Physical Stimulation , Rats , Rats, Inbred SHR , Rats, Inbred Strains , Rats, Inbred WKY , Vasodilation/drug effects , Vasodilator Agents/pharmacology
5.
Am J Physiol ; 274(6): R1613-8, 1998 06.
Article in English | MEDLINE | ID: mdl-9608015

ABSTRACT

Endothelin-1 (ET-1) is thought to play an important role in the development of deoxycorticosterone acetate (DOCA)-salt hypertension. Because hypertension is associated with an increased incidence of coronary artery disease, this study was designed to determine if coronary vascular contraction to ET-1 is altered in DOCA-salt hypertensive rats and to determine the effect of chronic treatment of DOCA-salt rats with the selective ETA receptor antagonist A-127722. Male Sprague-Dawley rats were divided into four groups: DOCA, Placebo, DOCA + A-127722, and Placebo + A-127722. A-127722 was administered in drinking water at a concentration of 8 mg/100 ml. After 3 wk, mean arterial pressure (MAP) was significantly enhanced in DOCA-salt compared with Placebo rats. A-127722 significantly inhibited the increase in MAP. Contraction to ET-1 (10(-11) to 3 x 10(-8) M) was measured in isolated coronary and mesenteric small arteries (200-300 micron, intraluminal diameter) maintained at a constant intraluminal pressure of 40 mmHg and was significantly impaired in vessels from DOCA-salt compared with Placebo rats. Dose-dependent contractions to KCl were also inhibited in coronary, but only minimally impaired in mesenteric, arteries of DOCA-salt rats. Inhibition of nitric oxide synthase activity did not restore contraction to ET-1 in coronary small arteries. However contractions to ET-1 were enhanced in mesenteric small arteries. Chronic treatment with A-127722 significantly restored contraction to ET-1 in coronary, but not in mesenteric, arteries of DOCA-salt rats. Because ETA receptor blockade impairs the development of hypertension and improves coronary vascular reactivity, these data indicate that ET-1 plays an important role in coronary vascular dysfunction associated with DOCA-salt hypertension.


Subject(s)
Coronary Vessels/metabolism , Coronary Vessels/physiopathology , Desoxycorticosterone , Endothelin Receptor Antagonists , Hypertension/chemically induced , Hypertension/physiopathology , Sodium Chloride , Animals , Atrasentan , Blood Pressure/drug effects , Coronary Vessels/drug effects , Dose-Response Relationship, Drug , Endothelin-1/pharmacology , Male , Mesenteric Arteries/drug effects , Potassium Chloride/pharmacology , Pyrrolidines/pharmacology , Rats , Rats, Sprague-Dawley , Receptor, Endothelin A , Vasoconstriction/drug effects
6.
J Pharmacol Exp Ther ; 282(3): 1643-9, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9316882

ABSTRACT

Basal release of endothelium-derived nitric oxide (NO) has been shown to modulate vascular tone and arterial pressure, and may be altered in disease states. The present study was designed to evaluate the role of nitric oxide synthase (NOS) in the maintenance of mean arterial pressure (MAP) and heart rate (HR) in early and advanced stages of cardiomyopathy. MAP and HR were measured via a carotid arterial cannula in conscious, unrestrained male Golden Syrian and Syrian cardiomyopathic hamsters. Studies were performed in young hamsters (age, 60-90 days) at the early phase and old hamsters (age, 300-350 days) at the advanced phase of cardiomyopathy. N-Nitro-L-arginine (LNA; 0.3-30 micromol/kg i.a.), an inhibitor of NOS activity, produced a dose-dependent increase in MAP in YC (young control) and OC (old control) hamsters. The LNA-induced increase in MAP was significantly impaired in YM (young cardiomyopathic) and was abolished in OM (old cardiomyopathic) hamsters compared with control hamsters. Bradycardia in response to LNA was similar in all groups. The effects of LNA on MAP and HR were reversed by L-arginine (200 mg/kg i.a.). Phenylephrine (0.3-300 microg/kg i.a.), an alpha adrenoceptor agonist, produced a dose-dependent increase in MAP which was similar in C and M hamsters at both ages, which indicated that impaired pressor responses to LNA were not caused by a nonspecific alteration in vascular responsiveness of M hamsters. Additionally, L-arginine (100 or 300 mg/kg i.a.), the precursor to NO and sodium nitroprusside (0.3-300 microg/kg i.a.), an NO donor, produced similar effects on MAP and HR in all groups of hamsters. Endothelial NOS protein levels in aorta isolated from each group of hamsters were similar. In the presence of tiron (1000 mg/kg), a superoxide anion scavenger, the effects of LNA on MAP were significantly restored in OM compared with OC hamsters. These results indicate that the role of NO in regulation of MAP is reduced during the development of cardiomyopathy. This effect is not the result of a deficiency of L-arginine, a reduced sensitivity to exogenous NO or a decrease in vascular endothelial NOS protein in cardiomyopathic hamsters. However, scavenging of NO by superoxide anions may contribute to the diminished role of NO in regulation of blood pressure in the advanced stage of cardiomyopathy.


Subject(s)
Blood Pressure , Cardiomyopathies/physiopathology , Nitric Oxide/physiology , Superoxides/metabolism , Animals , Blood Pressure/drug effects , Cricetinae , Dose-Response Relationship, Drug , Male , Mesocricetus , Nitroarginine/pharmacology , Nitroprusside/pharmacology
7.
Am J Physiol ; 273(1 Pt 2): H76-84, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9249477

ABSTRACT

Nitric oxide (NO) released from endothelial cells or exogenous nitrates is a potent dilator of arterial smooth muscle; however, the molecular mechanisms mediating relaxation to NO in the microcirculation have not been characterized. The present study investigated the relaxant effect of nitrovasodilators on microvessels obtained from the rat mesentery and also employed whole cell and single-channel patch-clamp techniques to identify the molecular target of NO action in myocytes from these vessels. Both sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) relaxed phenylephrine-induced contractions by approximately 80% but were significantly less effective in relaxing contractions induced by 40 mM KCl. Relaxation to SNP was also inhibited by the K(+)-channel blocker tetraethylammonium or by inhibition of the activity of the guanosine 3',5'-cyclic monophosphate (cGMP)-dependent protein kinase (PKG). These results suggest that SNP stimulated K+ efflux by opening K+ channels via PKG-mediated phosphorylation. Perforated-patch experiments revealed that both SNP and SNAP increased outward currents in microvascular myocytes, and single-channel studies identified the high-conductance Ca(2+)- and voltage-activated K+ (BKCa) channel as the target of nitrovasodilator action. The effects of nitrovasodilators on BKCa channels were mimicked by cGMP and inhibited by blocking the activity of PKG. We conclude that stimulation of BKCa-channel activity via cGMP-dependent phosphorylation contributes to the vasodilatory effect of NO on microvessels and that a direct effect of NO on BKCa channels does not play a major role in this process. We propose that this mechanism is important for the therapeutic effect of nitrovasodilators on peripheral resistance and arterial blood pressure.


Subject(s)
Cyclic GMP/analogs & derivatives , Cyclic GMP/physiology , Microcirculation/physiology , Muscle, Smooth, Vascular/physiology , Nitroprusside/pharmacology , Penicillamine/analogs & derivatives , Potassium Channels, Calcium-Activated , Potassium Channels/physiology , Splanchnic Circulation/physiology , Vasodilator Agents/pharmacology , Animals , Cells, Cultured , Cyclic GMP/pharmacology , Cyclic GMP-Dependent Protein Kinases/antagonists & inhibitors , In Vitro Techniques , Kinetics , Large-Conductance Calcium-Activated Potassium Channels , Male , Membrane Potentials/drug effects , Microcirculation/drug effects , Muscle Contraction/drug effects , Muscle Relaxation/drug effects , Muscle, Smooth, Vascular/drug effects , Patch-Clamp Techniques , Penicillamine/pharmacology , Phenylephrine/pharmacology , Potassium/metabolism , Potassium Channels/drug effects , Rats , Rats, Sprague-Dawley , S-Nitroso-N-Acetylpenicillamine , Splanchnic Circulation/drug effects , Tetraethylammonium , Tetraethylammonium Compounds/pharmacology , Time Factors
8.
Farmakol Toksikol ; 54(5): 20-2, 1991.
Article in Russian | MEDLINE | ID: mdl-1686868

ABSTRACT

The effects of yohimbine and idazoxane (0.5 and 5 mg/kg, intravenously) on the systemic and regional hemodynamics were studied in conscious Wistar rats by the microsphere technique. The both drugs induced an elevation of the arterial blood pressure and the heart rate. The administration of yohimbine and idazoxane in a dose of 0.5 mg/kg produced a more pronounced increase in the cerebral and coronary blood flow that a higher dose (5.0 mg/kg). The blood flow in the small intestine, kidneys and testis was decreased by a high dose of the studied alpha-blockers. It was suggested that the direct action of alpha 2-blockers on the vessel smooth muscle cells in responsible for the increase in the blood flow whereas blockade of alpha 2-receptors on the sympathetic terminals produces the decrease in the blood flow.


Subject(s)
Adrenergic alpha-Antagonists/pharmacology , Dioxanes/pharmacology , Hemodynamics/drug effects , Wakefulness/drug effects , Yohimbine/pharmacology , Animals , Dose-Response Relationship, Drug , Hemodynamics/physiology , Idazoxan , Microspheres , Rats , Rats, Inbred Strains , Wakefulness/physiology
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